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Immunomodulation of Oxidative Stress during Organ Donation Process: Preliminary Results

The objective was to quantify oxidative stress resulting from ischemia during the donation process, using malondialdehyde (MDA) measurement, and its modulation by the administration of melatonin. We designed a triple-blind clinical trial with donors randomized to melatonin or placebo. We collected d...

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Autores principales: Palomo-López, Nora, Rodríguez-Rodríguez, Ana, Martín-Villén, Luis, Mendoza-Prieto, María, Ruiz de Azúa-López, Zaida, Sempere-Bordes, Lluis, Boyero-Corral, Laura, Daga-Ruiz, Domingo, Gordillo-Brenes, Antonio, Pacheco-Sánchez, María, Perez-Villares, José Miguel, Vilches-Arenas, Ángel, Egea-Guerrero, Juan José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141959/
https://www.ncbi.nlm.nih.gov/pubmed/35627899
http://dx.doi.org/10.3390/healthcare10050762
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author Palomo-López, Nora
Rodríguez-Rodríguez, Ana
Martín-Villén, Luis
Mendoza-Prieto, María
Ruiz de Azúa-López, Zaida
Sempere-Bordes, Lluis
Boyero-Corral, Laura
Daga-Ruiz, Domingo
Gordillo-Brenes, Antonio
Pacheco-Sánchez, María
Perez-Villares, José Miguel
Vilches-Arenas, Ángel
Egea-Guerrero, Juan José
author_facet Palomo-López, Nora
Rodríguez-Rodríguez, Ana
Martín-Villén, Luis
Mendoza-Prieto, María
Ruiz de Azúa-López, Zaida
Sempere-Bordes, Lluis
Boyero-Corral, Laura
Daga-Ruiz, Domingo
Gordillo-Brenes, Antonio
Pacheco-Sánchez, María
Perez-Villares, José Miguel
Vilches-Arenas, Ángel
Egea-Guerrero, Juan José
author_sort Palomo-López, Nora
collection PubMed
description The objective was to quantify oxidative stress resulting from ischemia during the donation process, using malondialdehyde (MDA) measurement, and its modulation by the administration of melatonin. We designed a triple-blind clinical trial with donors randomized to melatonin or placebo. We collected donors by donation after brain death (DBD) and controlled donation after circulatory death (DCD), the latter maintained by normothermic regional perfusion (NRP). Melatonin or placebo was administered prior to donation or following limitation of therapeutic effort (LTE). Demographic variables and medical history were collected. We also collected serial measurements of MDA, at 60 and 90 min after melatonin or placebo administration. A total of 53 donors were included (32 from DBD and 21 from DCD). In the DBD group, 17 donors received melatonin, and 15 placebo. Eight DCD donors were randomized to melatonin and 13 to placebo. Medical history and cause for LTE were similar between groups. Although MDA values did not differ in the DBD group, statistical differences were observed in DCD donors during the 0–60 min interval: −4.296 (−6.752; −2.336) in the melatonin group and −1.612 (−2.886; −0.7445) in controls. Given the antioxidant effect of melatonin, its use could reduce the production of oxidative stress in controlled DCD.
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spelling pubmed-91419592022-05-28 Immunomodulation of Oxidative Stress during Organ Donation Process: Preliminary Results Palomo-López, Nora Rodríguez-Rodríguez, Ana Martín-Villén, Luis Mendoza-Prieto, María Ruiz de Azúa-López, Zaida Sempere-Bordes, Lluis Boyero-Corral, Laura Daga-Ruiz, Domingo Gordillo-Brenes, Antonio Pacheco-Sánchez, María Perez-Villares, José Miguel Vilches-Arenas, Ángel Egea-Guerrero, Juan José Healthcare (Basel) Article The objective was to quantify oxidative stress resulting from ischemia during the donation process, using malondialdehyde (MDA) measurement, and its modulation by the administration of melatonin. We designed a triple-blind clinical trial with donors randomized to melatonin or placebo. We collected donors by donation after brain death (DBD) and controlled donation after circulatory death (DCD), the latter maintained by normothermic regional perfusion (NRP). Melatonin or placebo was administered prior to donation or following limitation of therapeutic effort (LTE). Demographic variables and medical history were collected. We also collected serial measurements of MDA, at 60 and 90 min after melatonin or placebo administration. A total of 53 donors were included (32 from DBD and 21 from DCD). In the DBD group, 17 donors received melatonin, and 15 placebo. Eight DCD donors were randomized to melatonin and 13 to placebo. Medical history and cause for LTE were similar between groups. Although MDA values did not differ in the DBD group, statistical differences were observed in DCD donors during the 0–60 min interval: −4.296 (−6.752; −2.336) in the melatonin group and −1.612 (−2.886; −0.7445) in controls. Given the antioxidant effect of melatonin, its use could reduce the production of oxidative stress in controlled DCD. MDPI 2022-04-20 /pmc/articles/PMC9141959/ /pubmed/35627899 http://dx.doi.org/10.3390/healthcare10050762 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Palomo-López, Nora
Rodríguez-Rodríguez, Ana
Martín-Villén, Luis
Mendoza-Prieto, María
Ruiz de Azúa-López, Zaida
Sempere-Bordes, Lluis
Boyero-Corral, Laura
Daga-Ruiz, Domingo
Gordillo-Brenes, Antonio
Pacheco-Sánchez, María
Perez-Villares, José Miguel
Vilches-Arenas, Ángel
Egea-Guerrero, Juan José
Immunomodulation of Oxidative Stress during Organ Donation Process: Preliminary Results
title Immunomodulation of Oxidative Stress during Organ Donation Process: Preliminary Results
title_full Immunomodulation of Oxidative Stress during Organ Donation Process: Preliminary Results
title_fullStr Immunomodulation of Oxidative Stress during Organ Donation Process: Preliminary Results
title_full_unstemmed Immunomodulation of Oxidative Stress during Organ Donation Process: Preliminary Results
title_short Immunomodulation of Oxidative Stress during Organ Donation Process: Preliminary Results
title_sort immunomodulation of oxidative stress during organ donation process: preliminary results
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141959/
https://www.ncbi.nlm.nih.gov/pubmed/35627899
http://dx.doi.org/10.3390/healthcare10050762
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