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Preparation of Core-Shell Rare Earth-Doped Upconversion Nanomaterials and Simultaneous Detection of Two Pesticides in Food

Under the excitation of a 980 nm excitation light, the fluorescence signals of the synthesized core-shell NaYF(4):Yb@NaYF(4):Ho and monolayer NaYF(4):Yb,Tm upconversion nanoparticles (UCNPs) were simultaneously detected at 656 and 696 nm, respectively. The two upconversion materials were coupled wit...

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Detalles Bibliográficos
Autores principales: Zhu, Wenbo, Zhao, Lingyan, Jin, Jingyi, Song, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142009/
https://www.ncbi.nlm.nih.gov/pubmed/35627055
http://dx.doi.org/10.3390/foods11101485
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author Zhu, Wenbo
Zhao, Lingyan
Jin, Jingyi
Song, Yang
author_facet Zhu, Wenbo
Zhao, Lingyan
Jin, Jingyi
Song, Yang
author_sort Zhu, Wenbo
collection PubMed
description Under the excitation of a 980 nm excitation light, the fluorescence signals of the synthesized core-shell NaYF(4):Yb@NaYF(4):Ho and monolayer NaYF(4):Yb,Tm upconversion nanoparticles (UCNPs) were simultaneously detected at 656 and 696 nm, respectively. The two upconversion materials were coupled with anti-clothianidin and anti-imidacloprid monoclonal antibodies by the glutaraldehyde cross-linking method as signal probes. Imidacloprid (IMI) and clothianidin (CLO) could compete with antigen-conjugated amino Fe(3)O(4) magnetic nanomaterials for binding to signaling probes, thus establishing a rapid and sensitive fluorescent immunoassay for the simultaneous detection of IMI and CLO. Under optimal conditions, the limits of detection (LOD, IC(10)) and sensitivity (IC(50)) of IMI and CLO were (0.032, 0.028) and (4.7, 2.1) ng/mL, respectively, and the linear assay ranges were at 0.032–285.75 ng/mL and 0.028–200 ng/mL, respectively. Immunoassay did not cross-react significantly with other analogs. In fruits and vegetables such as apples, oranges, peaches, cucumbers, tomatoes and peppers, the mean recoveries of IMI and CLO ranged from 83.33% to 115.02% with relative standard deviations (RSDs) of 1.9% to 9.2% and 1.2% to 9.0%, respectively. Furthermore, the results of the immunoassay correlate well with the high-performance liquid chromatography method used to detect the actual samples.
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spelling pubmed-91420092022-05-28 Preparation of Core-Shell Rare Earth-Doped Upconversion Nanomaterials and Simultaneous Detection of Two Pesticides in Food Zhu, Wenbo Zhao, Lingyan Jin, Jingyi Song, Yang Foods Article Under the excitation of a 980 nm excitation light, the fluorescence signals of the synthesized core-shell NaYF(4):Yb@NaYF(4):Ho and monolayer NaYF(4):Yb,Tm upconversion nanoparticles (UCNPs) were simultaneously detected at 656 and 696 nm, respectively. The two upconversion materials were coupled with anti-clothianidin and anti-imidacloprid monoclonal antibodies by the glutaraldehyde cross-linking method as signal probes. Imidacloprid (IMI) and clothianidin (CLO) could compete with antigen-conjugated amino Fe(3)O(4) magnetic nanomaterials for binding to signaling probes, thus establishing a rapid and sensitive fluorescent immunoassay for the simultaneous detection of IMI and CLO. Under optimal conditions, the limits of detection (LOD, IC(10)) and sensitivity (IC(50)) of IMI and CLO were (0.032, 0.028) and (4.7, 2.1) ng/mL, respectively, and the linear assay ranges were at 0.032–285.75 ng/mL and 0.028–200 ng/mL, respectively. Immunoassay did not cross-react significantly with other analogs. In fruits and vegetables such as apples, oranges, peaches, cucumbers, tomatoes and peppers, the mean recoveries of IMI and CLO ranged from 83.33% to 115.02% with relative standard deviations (RSDs) of 1.9% to 9.2% and 1.2% to 9.0%, respectively. Furthermore, the results of the immunoassay correlate well with the high-performance liquid chromatography method used to detect the actual samples. MDPI 2022-05-19 /pmc/articles/PMC9142009/ /pubmed/35627055 http://dx.doi.org/10.3390/foods11101485 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhu, Wenbo
Zhao, Lingyan
Jin, Jingyi
Song, Yang
Preparation of Core-Shell Rare Earth-Doped Upconversion Nanomaterials and Simultaneous Detection of Two Pesticides in Food
title Preparation of Core-Shell Rare Earth-Doped Upconversion Nanomaterials and Simultaneous Detection of Two Pesticides in Food
title_full Preparation of Core-Shell Rare Earth-Doped Upconversion Nanomaterials and Simultaneous Detection of Two Pesticides in Food
title_fullStr Preparation of Core-Shell Rare Earth-Doped Upconversion Nanomaterials and Simultaneous Detection of Two Pesticides in Food
title_full_unstemmed Preparation of Core-Shell Rare Earth-Doped Upconversion Nanomaterials and Simultaneous Detection of Two Pesticides in Food
title_short Preparation of Core-Shell Rare Earth-Doped Upconversion Nanomaterials and Simultaneous Detection of Two Pesticides in Food
title_sort preparation of core-shell rare earth-doped upconversion nanomaterials and simultaneous detection of two pesticides in food
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142009/
https://www.ncbi.nlm.nih.gov/pubmed/35627055
http://dx.doi.org/10.3390/foods11101485
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