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Transcriptomic Analysis of the Spleen of Different Chicken Breeds Revealed the Differential Resistance of Salmonella Typhimurium

Salmonella Typhimurium (ST) is a foodborne pathogen that adversely affects the health of both animals and humans. Since poultry is a common source and carrier of the disease, controlling ST infection in chickens will have a protective impact on human health. In the current study, Beijing-You (BY) an...

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Autores principales: Elsharkawy, Mohamed Shafey, Wang, Hailong, Ding, Jiqiang, Madkour, Mahmoud, Wang, Qiao, Zhang, Qi, Zhang, Na, Li, Qinghe, Zhao, Guiping, Wen, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142047/
https://www.ncbi.nlm.nih.gov/pubmed/35627196
http://dx.doi.org/10.3390/genes13050811
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author Elsharkawy, Mohamed Shafey
Wang, Hailong
Ding, Jiqiang
Madkour, Mahmoud
Wang, Qiao
Zhang, Qi
Zhang, Na
Li, Qinghe
Zhao, Guiping
Wen, Jie
author_facet Elsharkawy, Mohamed Shafey
Wang, Hailong
Ding, Jiqiang
Madkour, Mahmoud
Wang, Qiao
Zhang, Qi
Zhang, Na
Li, Qinghe
Zhao, Guiping
Wen, Jie
author_sort Elsharkawy, Mohamed Shafey
collection PubMed
description Salmonella Typhimurium (ST) is a foodborne pathogen that adversely affects the health of both animals and humans. Since poultry is a common source and carrier of the disease, controlling ST infection in chickens will have a protective impact on human health. In the current study, Beijing-You (BY) and Cobb chicks (5-day-old specific-pathogen-free) were orally challenged by 2.4 × 10(12) CFU ST, spleen transcriptome was conducted 1 day post-infection (DPI) to identify gene markers and pathways related to the immune system. A total of 775 significant differentially expressed genes (DEGs) in comparisons between BY and Cobb were identified, including 498 upregulated and 277 downregulated genes (fold change ≥2.0, p < 0.05). Several immune response pathways against Salmonella were enriched, including natural killer-cell-mediated-cytotoxicity, cytokine–cytokine receptor interaction, antigen processing and presentation, phagosomes, and intestinal immune network for IgA production, for both BY and Cobb chickens. The BY chicks showed a robust response for clearance of bacterial load, immune response, and robust activation of phagosomes, resulting in ST resistance. These results confirmed that BY breed more resistance to ST challenge and will provide a better understanding of BY and Cobb chickens’ susceptibility and resistance to ST infection at the early stages of host immune response, which could expand the known intricacies of molecular mechanisms in chicken immunological responses against ST. Pathways induced by Salmonella infection may provide a novel approach to developing preventive and curative strategies for ST, and increase inherent resistance in animals through genetic selection.
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spelling pubmed-91420472022-05-28 Transcriptomic Analysis of the Spleen of Different Chicken Breeds Revealed the Differential Resistance of Salmonella Typhimurium Elsharkawy, Mohamed Shafey Wang, Hailong Ding, Jiqiang Madkour, Mahmoud Wang, Qiao Zhang, Qi Zhang, Na Li, Qinghe Zhao, Guiping Wen, Jie Genes (Basel) Article Salmonella Typhimurium (ST) is a foodborne pathogen that adversely affects the health of both animals and humans. Since poultry is a common source and carrier of the disease, controlling ST infection in chickens will have a protective impact on human health. In the current study, Beijing-You (BY) and Cobb chicks (5-day-old specific-pathogen-free) were orally challenged by 2.4 × 10(12) CFU ST, spleen transcriptome was conducted 1 day post-infection (DPI) to identify gene markers and pathways related to the immune system. A total of 775 significant differentially expressed genes (DEGs) in comparisons between BY and Cobb were identified, including 498 upregulated and 277 downregulated genes (fold change ≥2.0, p < 0.05). Several immune response pathways against Salmonella were enriched, including natural killer-cell-mediated-cytotoxicity, cytokine–cytokine receptor interaction, antigen processing and presentation, phagosomes, and intestinal immune network for IgA production, for both BY and Cobb chickens. The BY chicks showed a robust response for clearance of bacterial load, immune response, and robust activation of phagosomes, resulting in ST resistance. These results confirmed that BY breed more resistance to ST challenge and will provide a better understanding of BY and Cobb chickens’ susceptibility and resistance to ST infection at the early stages of host immune response, which could expand the known intricacies of molecular mechanisms in chicken immunological responses against ST. Pathways induced by Salmonella infection may provide a novel approach to developing preventive and curative strategies for ST, and increase inherent resistance in animals through genetic selection. MDPI 2022-05-02 /pmc/articles/PMC9142047/ /pubmed/35627196 http://dx.doi.org/10.3390/genes13050811 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elsharkawy, Mohamed Shafey
Wang, Hailong
Ding, Jiqiang
Madkour, Mahmoud
Wang, Qiao
Zhang, Qi
Zhang, Na
Li, Qinghe
Zhao, Guiping
Wen, Jie
Transcriptomic Analysis of the Spleen of Different Chicken Breeds Revealed the Differential Resistance of Salmonella Typhimurium
title Transcriptomic Analysis of the Spleen of Different Chicken Breeds Revealed the Differential Resistance of Salmonella Typhimurium
title_full Transcriptomic Analysis of the Spleen of Different Chicken Breeds Revealed the Differential Resistance of Salmonella Typhimurium
title_fullStr Transcriptomic Analysis of the Spleen of Different Chicken Breeds Revealed the Differential Resistance of Salmonella Typhimurium
title_full_unstemmed Transcriptomic Analysis of the Spleen of Different Chicken Breeds Revealed the Differential Resistance of Salmonella Typhimurium
title_short Transcriptomic Analysis of the Spleen of Different Chicken Breeds Revealed the Differential Resistance of Salmonella Typhimurium
title_sort transcriptomic analysis of the spleen of different chicken breeds revealed the differential resistance of salmonella typhimurium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142047/
https://www.ncbi.nlm.nih.gov/pubmed/35627196
http://dx.doi.org/10.3390/genes13050811
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