Cargando…
Higher-order olfactory neurons in the lateral horn support odor valence and odor identity coding in Drosophila
Understanding neuronal representations of odor-evoked activities and their progressive transformation from the sensory level to higher brain centers features one of the major aims in olfactory neuroscience. Here, we investigated how odor information is transformed and represented in higher-order neu...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142144/ https://www.ncbi.nlm.nih.gov/pubmed/35621267 http://dx.doi.org/10.7554/eLife.74637 |
_version_ | 1784715509810331648 |
---|---|
author | Das Chakraborty, Sudeshna Chang, Hetan Hansson, Bill S Sachse, Silke |
author_facet | Das Chakraborty, Sudeshna Chang, Hetan Hansson, Bill S Sachse, Silke |
author_sort | Das Chakraborty, Sudeshna |
collection | PubMed |
description | Understanding neuronal representations of odor-evoked activities and their progressive transformation from the sensory level to higher brain centers features one of the major aims in olfactory neuroscience. Here, we investigated how odor information is transformed and represented in higher-order neurons of the lateral horn, one of the higher olfactory centers implicated in determining innate behavior, using Drosophila melanogaster. We focused on a subset of third-order glutamatergic lateral horn neurons (LHNs) and characterized their odor coding properties in relation to their presynaptic partner neurons, the projection neurons (PNs) by two-photon functional imaging. We show that odors evoke reproducible, stereotypic, and odor-specific response patterns in LHNs. Notably, odor-evoked responses in these neurons are valence-specific in a way that their response amplitude is positively correlated with innate odor preferences. We postulate that this valence-specific activity is the result of integrating inputs from multiple olfactory channels through second-order neurons. GRASP and micro-lesioning experiments provide evidence that glutamatergic LHNs obtain their major excitatory input from uniglomerular PNs, while they receive an odor-specific inhibition through inhibitory multiglomerular PNs. In summary, our study indicates that odor representations in glutamatergic LHNs encode hedonic valence and odor identity and primarily retain the odor coding properties of second-order neurons. |
format | Online Article Text |
id | pubmed-9142144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-91421442022-05-28 Higher-order olfactory neurons in the lateral horn support odor valence and odor identity coding in Drosophila Das Chakraborty, Sudeshna Chang, Hetan Hansson, Bill S Sachse, Silke eLife Neuroscience Understanding neuronal representations of odor-evoked activities and their progressive transformation from the sensory level to higher brain centers features one of the major aims in olfactory neuroscience. Here, we investigated how odor information is transformed and represented in higher-order neurons of the lateral horn, one of the higher olfactory centers implicated in determining innate behavior, using Drosophila melanogaster. We focused on a subset of third-order glutamatergic lateral horn neurons (LHNs) and characterized their odor coding properties in relation to their presynaptic partner neurons, the projection neurons (PNs) by two-photon functional imaging. We show that odors evoke reproducible, stereotypic, and odor-specific response patterns in LHNs. Notably, odor-evoked responses in these neurons are valence-specific in a way that their response amplitude is positively correlated with innate odor preferences. We postulate that this valence-specific activity is the result of integrating inputs from multiple olfactory channels through second-order neurons. GRASP and micro-lesioning experiments provide evidence that glutamatergic LHNs obtain their major excitatory input from uniglomerular PNs, while they receive an odor-specific inhibition through inhibitory multiglomerular PNs. In summary, our study indicates that odor representations in glutamatergic LHNs encode hedonic valence and odor identity and primarily retain the odor coding properties of second-order neurons. eLife Sciences Publications, Ltd 2022-05-27 /pmc/articles/PMC9142144/ /pubmed/35621267 http://dx.doi.org/10.7554/eLife.74637 Text en © 2022, Das Chakraborty et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Das Chakraborty, Sudeshna Chang, Hetan Hansson, Bill S Sachse, Silke Higher-order olfactory neurons in the lateral horn support odor valence and odor identity coding in Drosophila |
title | Higher-order olfactory neurons in the lateral horn support odor valence and odor identity coding in Drosophila |
title_full | Higher-order olfactory neurons in the lateral horn support odor valence and odor identity coding in Drosophila |
title_fullStr | Higher-order olfactory neurons in the lateral horn support odor valence and odor identity coding in Drosophila |
title_full_unstemmed | Higher-order olfactory neurons in the lateral horn support odor valence and odor identity coding in Drosophila |
title_short | Higher-order olfactory neurons in the lateral horn support odor valence and odor identity coding in Drosophila |
title_sort | higher-order olfactory neurons in the lateral horn support odor valence and odor identity coding in drosophila |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142144/ https://www.ncbi.nlm.nih.gov/pubmed/35621267 http://dx.doi.org/10.7554/eLife.74637 |
work_keys_str_mv | AT daschakrabortysudeshna higherorderolfactoryneuronsinthelateralhornsupportodorvalenceandodoridentitycodingindrosophila AT changhetan higherorderolfactoryneuronsinthelateralhornsupportodorvalenceandodoridentitycodingindrosophila AT hanssonbills higherorderolfactoryneuronsinthelateralhornsupportodorvalenceandodoridentitycodingindrosophila AT sachsesilke higherorderolfactoryneuronsinthelateralhornsupportodorvalenceandodoridentitycodingindrosophila |