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The impact of microfluidics in high-throughput drug-screening applications

Drug discovery is an expensive and lengthy process. Among the different phases, drug discovery and preclinical trials play an important role as only 5–10 of all drugs that begin preclinical tests proceed to clinical trials. Indeed, current high-throughput screening technologies are very expensive, a...

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Detalles Bibliográficos
Autores principales: De Stefano, Paola, Bianchi, Elena, Dubini, Gabriele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142169/
https://www.ncbi.nlm.nih.gov/pubmed/35646223
http://dx.doi.org/10.1063/5.0087294
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author De Stefano, Paola
Bianchi, Elena
Dubini, Gabriele
author_facet De Stefano, Paola
Bianchi, Elena
Dubini, Gabriele
author_sort De Stefano, Paola
collection PubMed
description Drug discovery is an expensive and lengthy process. Among the different phases, drug discovery and preclinical trials play an important role as only 5–10 of all drugs that begin preclinical tests proceed to clinical trials. Indeed, current high-throughput screening technologies are very expensive, as they are unable to dispense small liquid volumes in an accurate and quick way. Moreover, despite being simple and fast, drug screening assays are usually performed under static conditions, thus failing to recapitulate tissue-specific architecture and biomechanical cues present in vivo even in the case of 3D models. On the contrary, microfluidics might offer a more rapid and cost-effective alternative. Although considered incompatible with high-throughput systems for years, technological advancements have demonstrated how this gap is rapidly reducing. In this Review, we want to further outline the role of microfluidics in high-throughput drug screening applications by looking at the multiple strategies for cell seeding, compartmentalization, continuous flow, stimuli administration (e.g., drug gradients or shear stresses), and single-cell analyses.
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spelling pubmed-91421692022-05-28 The impact of microfluidics in high-throughput drug-screening applications De Stefano, Paola Bianchi, Elena Dubini, Gabriele Biomicrofluidics Review Articles Drug discovery is an expensive and lengthy process. Among the different phases, drug discovery and preclinical trials play an important role as only 5–10 of all drugs that begin preclinical tests proceed to clinical trials. Indeed, current high-throughput screening technologies are very expensive, as they are unable to dispense small liquid volumes in an accurate and quick way. Moreover, despite being simple and fast, drug screening assays are usually performed under static conditions, thus failing to recapitulate tissue-specific architecture and biomechanical cues present in vivo even in the case of 3D models. On the contrary, microfluidics might offer a more rapid and cost-effective alternative. Although considered incompatible with high-throughput systems for years, technological advancements have demonstrated how this gap is rapidly reducing. In this Review, we want to further outline the role of microfluidics in high-throughput drug screening applications by looking at the multiple strategies for cell seeding, compartmentalization, continuous flow, stimuli administration (e.g., drug gradients or shear stresses), and single-cell analyses. AIP Publishing LLC 2022-05-26 /pmc/articles/PMC9142169/ /pubmed/35646223 http://dx.doi.org/10.1063/5.0087294 Text en © 2022 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Review Articles
De Stefano, Paola
Bianchi, Elena
Dubini, Gabriele
The impact of microfluidics in high-throughput drug-screening applications
title The impact of microfluidics in high-throughput drug-screening applications
title_full The impact of microfluidics in high-throughput drug-screening applications
title_fullStr The impact of microfluidics in high-throughput drug-screening applications
title_full_unstemmed The impact of microfluidics in high-throughput drug-screening applications
title_short The impact of microfluidics in high-throughput drug-screening applications
title_sort impact of microfluidics in high-throughput drug-screening applications
topic Review Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142169/
https://www.ncbi.nlm.nih.gov/pubmed/35646223
http://dx.doi.org/10.1063/5.0087294
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