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Internal oligo(dT) priming introduces systematic bias in bulk and single-cell RNA sequencing count data

Significant advances in RNA sequencing have been recently made possible by using oligo(dT) primers for simultaneous mRNA enrichment and reverse transcription priming. The associated increase in efficiency has enabled more economical bulk RNA sequencing methods and the advent of high-throughput singl...

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Detalles Bibliográficos
Autores principales:	Svoboda, Marek, Frost, H Robert, Bosco, Giovanni
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Methods Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142200/ https://www.ncbi.nlm.nih.gov/pubmed/35651651 http://dx.doi.org/10.1093/nargab/lqac035

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