Cargando…

Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches

The aim of this study was to investigate the phytochemical composition of dried Roselle calyx (Hibiscus sabdariffa L.) using both ethanolic and aqueous extracts. We report the antimicrobial activities against a wide range of bacteria, yeast, and fungi. The antioxidant activities were tested using 2,...

Descripción completa

Detalles Bibliográficos
Autores principales: Hamrita, Bechr, Emira, Noumi, Papetti, Adele, Badraoui, Riadh, Bouslama, Lamjed, Ben Tekfa, Mohamed-Iheb, Hamdi, Assia, Patel, Mitesh, Elasbali, Abdelbaset Mohamed, Adnan, Mohd, Ashraf, Syed Amir, Snoussi, Mejdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142284/
https://www.ncbi.nlm.nih.gov/pubmed/35646135
http://dx.doi.org/10.1155/2022/1252672
_version_ 1784715540173946880
author Hamrita, Bechr
Emira, Noumi
Papetti, Adele
Badraoui, Riadh
Bouslama, Lamjed
Ben Tekfa, Mohamed-Iheb
Hamdi, Assia
Patel, Mitesh
Elasbali, Abdelbaset Mohamed
Adnan, Mohd
Ashraf, Syed Amir
Snoussi, Mejdi
author_facet Hamrita, Bechr
Emira, Noumi
Papetti, Adele
Badraoui, Riadh
Bouslama, Lamjed
Ben Tekfa, Mohamed-Iheb
Hamdi, Assia
Patel, Mitesh
Elasbali, Abdelbaset Mohamed
Adnan, Mohd
Ashraf, Syed Amir
Snoussi, Mejdi
author_sort Hamrita, Bechr
collection PubMed
description The aim of this study was to investigate the phytochemical composition of dried Roselle calyx (Hibiscus sabdariffa L.) using both ethanolic and aqueous extracts. We report the antimicrobial activities against a wide range of bacteria, yeast, and fungi. The antioxidant activities were tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and 2–2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging assays. We report also for the first time the effect of the swarming motility in Pseudomonas aeruginosa PAO1. Our results showed that the tested two extracts were a rich source of phenols, flavonoids, and tannins with different degrees. Additionally, eleven phytoconstituents were identified by LC/MS technique (Hibiscus acid: 3-caffeoylquinic acid, 5-caffeoylquinic acid, 5-feruloylquinic acid, cyanidin 3-o-glucoside, myricetin, quercetin 7-o-rutinoside, quercetin 3-o-glucoside, delphinidin 3-o-sambubioside, and kaempferol 3-o-p-coumaroyl-glucoside). Also, it was shown that the calyx extract can scavenge 86% of the DPPH radical, while the rate of 53% and 23% of inhibition of the DPPH was obtained only at the concentration of 125 and 50 µg/mL, and a small inhibition was made at a concentration of 5 μg/mL. Roselle extracts inhibited the growth of the selected microorganisms at low concentrations, while higher concentrations are needed to completely kill them. However, no activity against CVB-3 was recorded for both extracts. In addition, the obtained extracts reduced the swarming motility of P. aeruginosa at 2.5 mg/ml. The docking simulation showed acceptable binding affinities (up to −9.6 kcal/mol) and interaction with key residues of 1JIJ, 2QZW, and 2UVO. The obtained results highlighted the potential use of Roselle extract as a source of phytoconstituents with promising antimicrobial, antioxidant, and anti-quorum sensing activities.
format Online
Article
Text
id pubmed-9142284
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-91422842022-05-28 Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches Hamrita, Bechr Emira, Noumi Papetti, Adele Badraoui, Riadh Bouslama, Lamjed Ben Tekfa, Mohamed-Iheb Hamdi, Assia Patel, Mitesh Elasbali, Abdelbaset Mohamed Adnan, Mohd Ashraf, Syed Amir Snoussi, Mejdi Evid Based Complement Alternat Med Research Article The aim of this study was to investigate the phytochemical composition of dried Roselle calyx (Hibiscus sabdariffa L.) using both ethanolic and aqueous extracts. We report the antimicrobial activities against a wide range of bacteria, yeast, and fungi. The antioxidant activities were tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and 2–2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging assays. We report also for the first time the effect of the swarming motility in Pseudomonas aeruginosa PAO1. Our results showed that the tested two extracts were a rich source of phenols, flavonoids, and tannins with different degrees. Additionally, eleven phytoconstituents were identified by LC/MS technique (Hibiscus acid: 3-caffeoylquinic acid, 5-caffeoylquinic acid, 5-feruloylquinic acid, cyanidin 3-o-glucoside, myricetin, quercetin 7-o-rutinoside, quercetin 3-o-glucoside, delphinidin 3-o-sambubioside, and kaempferol 3-o-p-coumaroyl-glucoside). Also, it was shown that the calyx extract can scavenge 86% of the DPPH radical, while the rate of 53% and 23% of inhibition of the DPPH was obtained only at the concentration of 125 and 50 µg/mL, and a small inhibition was made at a concentration of 5 μg/mL. Roselle extracts inhibited the growth of the selected microorganisms at low concentrations, while higher concentrations are needed to completely kill them. However, no activity against CVB-3 was recorded for both extracts. In addition, the obtained extracts reduced the swarming motility of P. aeruginosa at 2.5 mg/ml. The docking simulation showed acceptable binding affinities (up to −9.6 kcal/mol) and interaction with key residues of 1JIJ, 2QZW, and 2UVO. The obtained results highlighted the potential use of Roselle extract as a source of phytoconstituents with promising antimicrobial, antioxidant, and anti-quorum sensing activities. Hindawi 2022-05-20 /pmc/articles/PMC9142284/ /pubmed/35646135 http://dx.doi.org/10.1155/2022/1252672 Text en Copyright © 2022 Bechr Hamrita et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hamrita, Bechr
Emira, Noumi
Papetti, Adele
Badraoui, Riadh
Bouslama, Lamjed
Ben Tekfa, Mohamed-Iheb
Hamdi, Assia
Patel, Mitesh
Elasbali, Abdelbaset Mohamed
Adnan, Mohd
Ashraf, Syed Amir
Snoussi, Mejdi
Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches
title Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches
title_full Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches
title_fullStr Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches
title_full_unstemmed Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches
title_short Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches
title_sort phytochemical analysis, antioxidant, antimicrobial, and anti-swarming properties of hibiscus sabdariffa l. calyx extracts: in vitro and in silico modelling approaches
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142284/
https://www.ncbi.nlm.nih.gov/pubmed/35646135
http://dx.doi.org/10.1155/2022/1252672
work_keys_str_mv AT hamritabechr phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT emiranoumi phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT papettiadele phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT badraouiriadh phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT bouslamalamjed phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT bentekfamohamediheb phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT hamdiassia phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT patelmitesh phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT elasbaliabdelbasetmohamed phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT adnanmohd phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT ashrafsyedamir phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches
AT snoussimejdi phytochemicalanalysisantioxidantantimicrobialandantiswarmingpropertiesofhibiscussabdariffalcalyxextractsinvitroandinsilicomodellingapproaches