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Impact of optogenetic pulse design on CA3 learning and replay: A neural model
Optogenetic manipulation of hippocampal circuitry is an important tool for investigating learning in vivo. Numerous approaches to pulse design have been employed to elicit desirable circuit and behavioral outcomes. Here, we systematically test the outcome of different single-pulse waveforms in a rat...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142690/ https://www.ncbi.nlm.nih.gov/pubmed/35637904 http://dx.doi.org/10.1016/j.crmeth.2022.100208 |
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author | Wilmerding, Lucius K. Yazdanbakhsh, Arash Hasselmo, Michael E. |
author_facet | Wilmerding, Lucius K. Yazdanbakhsh, Arash Hasselmo, Michael E. |
author_sort | Wilmerding, Lucius K. |
collection | PubMed |
description | Optogenetic manipulation of hippocampal circuitry is an important tool for investigating learning in vivo. Numerous approaches to pulse design have been employed to elicit desirable circuit and behavioral outcomes. Here, we systematically test the outcome of different single-pulse waveforms in a rate-based model of hippocampal memory function at the level of mnemonic replay extension and de novo synaptic weight formation in CA3 and CA1. Lower-power waveforms with long forward or forward and backward ramps yield more natural sequence replay dynamics and induce synaptic plasticity that allows for more natural memory replay timing, in contrast to square or backward ramps. These differences between waveform shape and amplitude are preserved with the addition of noise in membrane potential, light scattering, and protein expression, improving the potential validity of predictions for in vivo work. These results inform future optogenetic experimental design choices in the field of learning and memory. |
format | Online Article Text |
id | pubmed-9142690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-91426902022-05-29 Impact of optogenetic pulse design on CA3 learning and replay: A neural model Wilmerding, Lucius K. Yazdanbakhsh, Arash Hasselmo, Michael E. Cell Rep Methods Resource Optogenetic manipulation of hippocampal circuitry is an important tool for investigating learning in vivo. Numerous approaches to pulse design have been employed to elicit desirable circuit and behavioral outcomes. Here, we systematically test the outcome of different single-pulse waveforms in a rate-based model of hippocampal memory function at the level of mnemonic replay extension and de novo synaptic weight formation in CA3 and CA1. Lower-power waveforms with long forward or forward and backward ramps yield more natural sequence replay dynamics and induce synaptic plasticity that allows for more natural memory replay timing, in contrast to square or backward ramps. These differences between waveform shape and amplitude are preserved with the addition of noise in membrane potential, light scattering, and protein expression, improving the potential validity of predictions for in vivo work. These results inform future optogenetic experimental design choices in the field of learning and memory. Elsevier 2022-05-03 /pmc/articles/PMC9142690/ /pubmed/35637904 http://dx.doi.org/10.1016/j.crmeth.2022.100208 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Resource Wilmerding, Lucius K. Yazdanbakhsh, Arash Hasselmo, Michael E. Impact of optogenetic pulse design on CA3 learning and replay: A neural model |
title | Impact of optogenetic pulse design on CA3 learning and replay: A neural model |
title_full | Impact of optogenetic pulse design on CA3 learning and replay: A neural model |
title_fullStr | Impact of optogenetic pulse design on CA3 learning and replay: A neural model |
title_full_unstemmed | Impact of optogenetic pulse design on CA3 learning and replay: A neural model |
title_short | Impact of optogenetic pulse design on CA3 learning and replay: A neural model |
title_sort | impact of optogenetic pulse design on ca3 learning and replay: a neural model |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142690/ https://www.ncbi.nlm.nih.gov/pubmed/35637904 http://dx.doi.org/10.1016/j.crmeth.2022.100208 |
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