Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron

The SARS-CoV-2 nucleocapsid protein (NP) is an important indicator for the virus infection, highlighting the crucial role of NP-specific monoclonal antibodies (mAbs) used in multiple biochemical assays and clinical diagnosis for detecting the NP antigen. Here, we reported a pair of noncompeting huma...

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Autores principales: Zhou, Bing, Cheng, Lin, Song, Shuo, Guo, Huimin, Shen, Senlin, Wang, Haiyan, Ge, Xiangyang, Liu, Lei, Ju, Bin, Zhang, Zheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142731/
https://www.ncbi.nlm.nih.gov/pubmed/35643510
http://dx.doi.org/10.1186/s12985-022-01827-w
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author Zhou, Bing
Cheng, Lin
Song, Shuo
Guo, Huimin
Shen, Senlin
Wang, Haiyan
Ge, Xiangyang
Liu, Lei
Ju, Bin
Zhang, Zheng
author_facet Zhou, Bing
Cheng, Lin
Song, Shuo
Guo, Huimin
Shen, Senlin
Wang, Haiyan
Ge, Xiangyang
Liu, Lei
Ju, Bin
Zhang, Zheng
author_sort Zhou, Bing
collection PubMed
description The SARS-CoV-2 nucleocapsid protein (NP) is an important indicator for the virus infection, highlighting the crucial role of NP-specific monoclonal antibodies (mAbs) used in multiple biochemical assays and clinical diagnosis for detecting the NP antigen. Here, we reported a pair of noncompeting human NP-specific mAbs, named P301-F7 and P301-H5, targeting two distinct linear epitopes on SARS-CoV-2 or SARS-CoV. We evaluated the application of P301-F7 in the analysis of enzyme linked immunosorbent assay, western blot, flow cytometry, immunofluorescence, and focus reduction neutralization test. We for the first time report a broad mAb effectively recognizing various live viruses of SARS-CoV-2 variants including Alpha, Beta, Delta, and Omicron, indicating a wide range of application prospects. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-022-01827-w.
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spelling pubmed-91427312022-05-29 Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron Zhou, Bing Cheng, Lin Song, Shuo Guo, Huimin Shen, Senlin Wang, Haiyan Ge, Xiangyang Liu, Lei Ju, Bin Zhang, Zheng Virol J Brief Report The SARS-CoV-2 nucleocapsid protein (NP) is an important indicator for the virus infection, highlighting the crucial role of NP-specific monoclonal antibodies (mAbs) used in multiple biochemical assays and clinical diagnosis for detecting the NP antigen. Here, we reported a pair of noncompeting human NP-specific mAbs, named P301-F7 and P301-H5, targeting two distinct linear epitopes on SARS-CoV-2 or SARS-CoV. We evaluated the application of P301-F7 in the analysis of enzyme linked immunosorbent assay, western blot, flow cytometry, immunofluorescence, and focus reduction neutralization test. We for the first time report a broad mAb effectively recognizing various live viruses of SARS-CoV-2 variants including Alpha, Beta, Delta, and Omicron, indicating a wide range of application prospects. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-022-01827-w. BioMed Central 2022-05-28 /pmc/articles/PMC9142731/ /pubmed/35643510 http://dx.doi.org/10.1186/s12985-022-01827-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Brief Report
Zhou, Bing
Cheng, Lin
Song, Shuo
Guo, Huimin
Shen, Senlin
Wang, Haiyan
Ge, Xiangyang
Liu, Lei
Ju, Bin
Zhang, Zheng
Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron
title Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron
title_full Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron
title_fullStr Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron
title_full_unstemmed Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron
title_short Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron
title_sort identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of sars-cov-2 variants including omicron
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142731/
https://www.ncbi.nlm.nih.gov/pubmed/35643510
http://dx.doi.org/10.1186/s12985-022-01827-w
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