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Felodipine Determination by a CdTe Quantum Dot-Based Fluorescent Probe
In this work, a CdTe quantum dot-based fluorescent probe was synthesized to determine felodipine (FEL). The synthesis conditions, structure, and interaction conditions with FEL of CdTe quantum dots were analysed by fluorescence spectrophotometry, Fourier transform infrared spectroscopy (FT-IR), X-ra...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142910/ https://www.ncbi.nlm.nih.gov/pubmed/35630254 http://dx.doi.org/10.3390/mi13050788 |
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author | Lv, Yuguang Cheng, Yuqing Lv, Kuilin Zhang, Guoliang Wu, Jiang |
author_facet | Lv, Yuguang Cheng, Yuqing Lv, Kuilin Zhang, Guoliang Wu, Jiang |
author_sort | Lv, Yuguang |
collection | PubMed |
description | In this work, a CdTe quantum dot-based fluorescent probe was synthesized to determine felodipine (FEL). The synthesis conditions, structure, and interaction conditions with FEL of CdTe quantum dots were analysed by fluorescence spectrophotometry, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), UV–visible spectroscopy, and TEM. The CdTe QD concentration was 2.0 × 10(−4) mol/L. The amount of quantum dots controlled in the experiment was 0.8 mL. The controlled feeding ratio of N (Cd(2+)):N (Te(2−)):N (TGA) was 2:1:4, the heating temperature was 140 °C, the heating time was 60 min, and the pH of the QD precursor was adjusted to 11 for subsequent experiments. The UV–visible spectrum showed that the emission wavelength of CdTe quantum dots at 545 nm was the strongest and symmetric. The particle size of the synthesized quantum dots was approximately 5 nm. In the interaction of CdTe quantum dots with FEL, the FEL dosage was 1.0 mL, the optimal pH value of Tris-HCl buffer was 8.2, the amount of buffer was 1.5 mL, and the reaction time was 20 min. The standard curve of FEL was determined under the optimal synthesis conditions of CdTe quantum dots and reaction of CdTe quantum dots with FEL. The linear equation was Y = 3.9448x + 50.068, the correlation coefficient R2 was 0.9986, and the linear range was 5 × 10(−6)–1.1 × 10(−4) mol/L. A CdTe quantum dot-based fluorescent probe was successfully constructed and could be used to determine the FEL tablet content. |
format | Online Article Text |
id | pubmed-9142910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91429102022-05-29 Felodipine Determination by a CdTe Quantum Dot-Based Fluorescent Probe Lv, Yuguang Cheng, Yuqing Lv, Kuilin Zhang, Guoliang Wu, Jiang Micromachines (Basel) Article In this work, a CdTe quantum dot-based fluorescent probe was synthesized to determine felodipine (FEL). The synthesis conditions, structure, and interaction conditions with FEL of CdTe quantum dots were analysed by fluorescence spectrophotometry, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), UV–visible spectroscopy, and TEM. The CdTe QD concentration was 2.0 × 10(−4) mol/L. The amount of quantum dots controlled in the experiment was 0.8 mL. The controlled feeding ratio of N (Cd(2+)):N (Te(2−)):N (TGA) was 2:1:4, the heating temperature was 140 °C, the heating time was 60 min, and the pH of the QD precursor was adjusted to 11 for subsequent experiments. The UV–visible spectrum showed that the emission wavelength of CdTe quantum dots at 545 nm was the strongest and symmetric. The particle size of the synthesized quantum dots was approximately 5 nm. In the interaction of CdTe quantum dots with FEL, the FEL dosage was 1.0 mL, the optimal pH value of Tris-HCl buffer was 8.2, the amount of buffer was 1.5 mL, and the reaction time was 20 min. The standard curve of FEL was determined under the optimal synthesis conditions of CdTe quantum dots and reaction of CdTe quantum dots with FEL. The linear equation was Y = 3.9448x + 50.068, the correlation coefficient R2 was 0.9986, and the linear range was 5 × 10(−6)–1.1 × 10(−4) mol/L. A CdTe quantum dot-based fluorescent probe was successfully constructed and could be used to determine the FEL tablet content. MDPI 2022-05-18 /pmc/articles/PMC9142910/ /pubmed/35630254 http://dx.doi.org/10.3390/mi13050788 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lv, Yuguang Cheng, Yuqing Lv, Kuilin Zhang, Guoliang Wu, Jiang Felodipine Determination by a CdTe Quantum Dot-Based Fluorescent Probe |
title | Felodipine Determination by a CdTe Quantum Dot-Based Fluorescent Probe |
title_full | Felodipine Determination by a CdTe Quantum Dot-Based Fluorescent Probe |
title_fullStr | Felodipine Determination by a CdTe Quantum Dot-Based Fluorescent Probe |
title_full_unstemmed | Felodipine Determination by a CdTe Quantum Dot-Based Fluorescent Probe |
title_short | Felodipine Determination by a CdTe Quantum Dot-Based Fluorescent Probe |
title_sort | felodipine determination by a cdte quantum dot-based fluorescent probe |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142910/ https://www.ncbi.nlm.nih.gov/pubmed/35630254 http://dx.doi.org/10.3390/mi13050788 |
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