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Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine
Porcine circovirus type 2 (PCV2) is responsible for a number of porcine circovirus-associated diseases (PCVAD) that can severely impact domestic pig herds. For a non-enveloped virus with a small genome (1.7 kb ssDNA), PCV2 is remarkably diverse, with eight genotypes (a–h). New genotypes of PCV2 can...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142977/ https://www.ncbi.nlm.nih.gov/pubmed/35632666 http://dx.doi.org/10.3390/v14050924 |
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author | Rudova, Nataliia Buttler, Jeremy Kovalenko, Ganna Sushko, Mykola Bolotin, Vitaliy Muzykina, Larysa Zinenko, Oleksandr Stegniy, Borys Dunaiev, Yurii Sytiuk, Mykola Gerilovych, Anton Drown, Devin M. Bortz, Eric Solodiankin, Oleksii |
author_facet | Rudova, Nataliia Buttler, Jeremy Kovalenko, Ganna Sushko, Mykola Bolotin, Vitaliy Muzykina, Larysa Zinenko, Oleksandr Stegniy, Borys Dunaiev, Yurii Sytiuk, Mykola Gerilovych, Anton Drown, Devin M. Bortz, Eric Solodiankin, Oleksii |
author_sort | Rudova, Nataliia |
collection | PubMed |
description | Porcine circovirus type 2 (PCV2) is responsible for a number of porcine circovirus-associated diseases (PCVAD) that can severely impact domestic pig herds. For a non-enveloped virus with a small genome (1.7 kb ssDNA), PCV2 is remarkably diverse, with eight genotypes (a–h). New genotypes of PCV2 can spread through the migration of wild boar, which are thought to infect domestic pigs and spread further through the domestic pig trade. Despite a large swine population, the diversity of PCV2 genotypes in Ukraine has been under-sampled, with few PCV2 genome sequences reported in the past decade. To gain a deeper understanding of PCV2 genotype diversity in Ukraine, samples of blood serum were collected from wild boars (n = 107) that were hunted in Ukraine during the November–December 2012 hunting season. We found 34/107 (31.8%) prevalence of PCV2 by diagnostic PCR. For domestic pigs, liver samples (n = 16) were collected from a commercial market near Kharkiv in 2019, of which 6 out of 16 (37%) samples were positive for PCV2. We sequenced the genotyping locus ORF2, a gene encoding the PCV2 viral capsid (Cap), for 11 wild boar and six domestic pig samples in Ukraine using an Oxford Nanopore MinION device. Of 17 samples with resolved genotypes, the PCV2 genotype b was the most common in wild boar samples (10 out of 11, 91%), while the domestic pigs were infected with genotypes b and d. We also detected genotype b/d and b/a co-infections in wild boars and domestic pigs, respectively, and for the first time in Ukraine we detected genotype f in a wild boar from Poltava. Building a maximum-likelihood phylogeny, we identified a sublineage of PCV2 genotype b infections in both wild and domestic swine, suggesting a possible epizootic cluster and an ecological interaction between wild boar and domestic pig populations in northeastern Ukraine. |
format | Online Article Text |
id | pubmed-9142977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91429772022-05-29 Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine Rudova, Nataliia Buttler, Jeremy Kovalenko, Ganna Sushko, Mykola Bolotin, Vitaliy Muzykina, Larysa Zinenko, Oleksandr Stegniy, Borys Dunaiev, Yurii Sytiuk, Mykola Gerilovych, Anton Drown, Devin M. Bortz, Eric Solodiankin, Oleksii Viruses Article Porcine circovirus type 2 (PCV2) is responsible for a number of porcine circovirus-associated diseases (PCVAD) that can severely impact domestic pig herds. For a non-enveloped virus with a small genome (1.7 kb ssDNA), PCV2 is remarkably diverse, with eight genotypes (a–h). New genotypes of PCV2 can spread through the migration of wild boar, which are thought to infect domestic pigs and spread further through the domestic pig trade. Despite a large swine population, the diversity of PCV2 genotypes in Ukraine has been under-sampled, with few PCV2 genome sequences reported in the past decade. To gain a deeper understanding of PCV2 genotype diversity in Ukraine, samples of blood serum were collected from wild boars (n = 107) that were hunted in Ukraine during the November–December 2012 hunting season. We found 34/107 (31.8%) prevalence of PCV2 by diagnostic PCR. For domestic pigs, liver samples (n = 16) were collected from a commercial market near Kharkiv in 2019, of which 6 out of 16 (37%) samples were positive for PCV2. We sequenced the genotyping locus ORF2, a gene encoding the PCV2 viral capsid (Cap), for 11 wild boar and six domestic pig samples in Ukraine using an Oxford Nanopore MinION device. Of 17 samples with resolved genotypes, the PCV2 genotype b was the most common in wild boar samples (10 out of 11, 91%), while the domestic pigs were infected with genotypes b and d. We also detected genotype b/d and b/a co-infections in wild boars and domestic pigs, respectively, and for the first time in Ukraine we detected genotype f in a wild boar from Poltava. Building a maximum-likelihood phylogeny, we identified a sublineage of PCV2 genotype b infections in both wild and domestic swine, suggesting a possible epizootic cluster and an ecological interaction between wild boar and domestic pig populations in northeastern Ukraine. MDPI 2022-04-28 /pmc/articles/PMC9142977/ /pubmed/35632666 http://dx.doi.org/10.3390/v14050924 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rudova, Nataliia Buttler, Jeremy Kovalenko, Ganna Sushko, Mykola Bolotin, Vitaliy Muzykina, Larysa Zinenko, Oleksandr Stegniy, Borys Dunaiev, Yurii Sytiuk, Mykola Gerilovych, Anton Drown, Devin M. Bortz, Eric Solodiankin, Oleksii Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine |
title | Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine |
title_full | Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine |
title_fullStr | Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine |
title_full_unstemmed | Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine |
title_short | Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine |
title_sort | genetic diversity of porcine circovirus 2 in wild boar and domestic pigs in ukraine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142977/ https://www.ncbi.nlm.nih.gov/pubmed/35632666 http://dx.doi.org/10.3390/v14050924 |
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