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Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers

Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However,...

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Autores principales: Korniienko, Viktoriia, Husak, Yevheniia, Radwan-Pragłowska, Julia, Holubnycha, Viktoriia, Samokhin, Yevhen, Yanovska, Anna, Varava, Julia, Diedkova, Kateryna, Janus, Łukasz, Pogorielov, Maksym
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142982/
https://www.ncbi.nlm.nih.gov/pubmed/35630820
http://dx.doi.org/10.3390/molecules27103343
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author Korniienko, Viktoriia
Husak, Yevheniia
Radwan-Pragłowska, Julia
Holubnycha, Viktoriia
Samokhin, Yevhen
Yanovska, Anna
Varava, Julia
Diedkova, Kateryna
Janus, Łukasz
Pogorielov, Maksym
author_facet Korniienko, Viktoriia
Husak, Yevheniia
Radwan-Pragłowska, Julia
Holubnycha, Viktoriia
Samokhin, Yevhen
Yanovska, Anna
Varava, Julia
Diedkova, Kateryna
Janus, Łukasz
Pogorielov, Maksym
author_sort Korniienko, Viktoriia
collection PubMed
description Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using Staphylococcus aureus and Escherichia coli as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both E. coli and S. aureus in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism.
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spelling pubmed-91429822022-05-29 Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers Korniienko, Viktoriia Husak, Yevheniia Radwan-Pragłowska, Julia Holubnycha, Viktoriia Samokhin, Yevhen Yanovska, Anna Varava, Julia Diedkova, Kateryna Janus, Łukasz Pogorielov, Maksym Molecules Article Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using Staphylococcus aureus and Escherichia coli as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both E. coli and S. aureus in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism. MDPI 2022-05-23 /pmc/articles/PMC9142982/ /pubmed/35630820 http://dx.doi.org/10.3390/molecules27103343 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Korniienko, Viktoriia
Husak, Yevheniia
Radwan-Pragłowska, Julia
Holubnycha, Viktoriia
Samokhin, Yevhen
Yanovska, Anna
Varava, Julia
Diedkova, Kateryna
Janus, Łukasz
Pogorielov, Maksym
Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers
title Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers
title_full Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers
title_fullStr Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers
title_full_unstemmed Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers
title_short Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers
title_sort impact of electrospinning parameters and post-treatment method on antibacterial and antibiofilm activity of chitosan nanofibers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142982/
https://www.ncbi.nlm.nih.gov/pubmed/35630820
http://dx.doi.org/10.3390/molecules27103343
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