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Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers
Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However,...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142982/ https://www.ncbi.nlm.nih.gov/pubmed/35630820 http://dx.doi.org/10.3390/molecules27103343 |
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author | Korniienko, Viktoriia Husak, Yevheniia Radwan-Pragłowska, Julia Holubnycha, Viktoriia Samokhin, Yevhen Yanovska, Anna Varava, Julia Diedkova, Kateryna Janus, Łukasz Pogorielov, Maksym |
author_facet | Korniienko, Viktoriia Husak, Yevheniia Radwan-Pragłowska, Julia Holubnycha, Viktoriia Samokhin, Yevhen Yanovska, Anna Varava, Julia Diedkova, Kateryna Janus, Łukasz Pogorielov, Maksym |
author_sort | Korniienko, Viktoriia |
collection | PubMed |
description | Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using Staphylococcus aureus and Escherichia coli as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both E. coli and S. aureus in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism. |
format | Online Article Text |
id | pubmed-9142982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91429822022-05-29 Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers Korniienko, Viktoriia Husak, Yevheniia Radwan-Pragłowska, Julia Holubnycha, Viktoriia Samokhin, Yevhen Yanovska, Anna Varava, Julia Diedkova, Kateryna Janus, Łukasz Pogorielov, Maksym Molecules Article Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using Staphylococcus aureus and Escherichia coli as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both E. coli and S. aureus in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism. MDPI 2022-05-23 /pmc/articles/PMC9142982/ /pubmed/35630820 http://dx.doi.org/10.3390/molecules27103343 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Korniienko, Viktoriia Husak, Yevheniia Radwan-Pragłowska, Julia Holubnycha, Viktoriia Samokhin, Yevhen Yanovska, Anna Varava, Julia Diedkova, Kateryna Janus, Łukasz Pogorielov, Maksym Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
title | Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
title_full | Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
title_fullStr | Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
title_full_unstemmed | Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
title_short | Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers |
title_sort | impact of electrospinning parameters and post-treatment method on antibacterial and antibiofilm activity of chitosan nanofibers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142982/ https://www.ncbi.nlm.nih.gov/pubmed/35630820 http://dx.doi.org/10.3390/molecules27103343 |
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