Cargando…
Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors
Molecular docking revealed curcumin as a potent CB2 cannabinoid receptor (CB2R) agonist. Since CB2R is involved in cardioprotective functions, we explored its role in ameliorative actions of curcumin against myocardial damage triggered by isoproterenol in diabetic animals. Mice were kept on a high-f...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143027/ https://www.ncbi.nlm.nih.gov/pubmed/35629293 http://dx.doi.org/10.3390/life12050624 |
_version_ | 1784715704223662080 |
---|---|
author | Pawar, Harshal D. Mahajan, Umesh B. Nakhate, Kartik T. Agrawal, Yogeeta O. Patil, Chandragouda R. Meeran, M. F. Nagoor Sharma, Charu Ojha, Shreesh Goyal, Sameer N. |
author_facet | Pawar, Harshal D. Mahajan, Umesh B. Nakhate, Kartik T. Agrawal, Yogeeta O. Patil, Chandragouda R. Meeran, M. F. Nagoor Sharma, Charu Ojha, Shreesh Goyal, Sameer N. |
author_sort | Pawar, Harshal D. |
collection | PubMed |
description | Molecular docking revealed curcumin as a potent CB2 cannabinoid receptor (CB2R) agonist. Since CB2R is involved in cardioprotective functions, we explored its role in ameliorative actions of curcumin against myocardial damage triggered by isoproterenol in diabetic animals. Mice were kept on a high-fat diet (HFD) throughout the experiment (30 days). Following 7 days of HFD feeding, streptozotocin was administered (150 mg/kg, intraperitoneal) to induce diabetes. From day 11 to 30, diabetic mice received either curcumin (100 or 200 mg/kg/day, oral), CB2R antagonist AM630 (1 mg/kg/day, intraperitoneal) or both, with concurrent isoproterenol (150 mg/kg, subcutaneous) administration on day 28 and 29. Diabetic mice with myocardial infarction showed an altered hemodynamic pattern and lipid profile, reduced injury markers, antioxidants with increased lipid peroxidation in the myocardium, and elevated glucose and liver enzymes in the blood. Moreover, an increased pro-inflammatory markers, histological severity, myonecrosis, and edema were observed. Curcumin compensated for hemodynamic fluctuations, restored biochemical markers, preserved antioxidant capacity, decreased cytokines levels, and restored cardiac functionality. However, the AM630 pre-treatment attenuated the effects of curcumin. The data suggest the involvement of CB2R in the actions of curcumin such as in the prevention of myocardial stress and in the improvement of the normal status of the myocardial membrane associated with diabetes. |
format | Online Article Text |
id | pubmed-9143027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91430272022-05-29 Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors Pawar, Harshal D. Mahajan, Umesh B. Nakhate, Kartik T. Agrawal, Yogeeta O. Patil, Chandragouda R. Meeran, M. F. Nagoor Sharma, Charu Ojha, Shreesh Goyal, Sameer N. Life (Basel) Article Molecular docking revealed curcumin as a potent CB2 cannabinoid receptor (CB2R) agonist. Since CB2R is involved in cardioprotective functions, we explored its role in ameliorative actions of curcumin against myocardial damage triggered by isoproterenol in diabetic animals. Mice were kept on a high-fat diet (HFD) throughout the experiment (30 days). Following 7 days of HFD feeding, streptozotocin was administered (150 mg/kg, intraperitoneal) to induce diabetes. From day 11 to 30, diabetic mice received either curcumin (100 or 200 mg/kg/day, oral), CB2R antagonist AM630 (1 mg/kg/day, intraperitoneal) or both, with concurrent isoproterenol (150 mg/kg, subcutaneous) administration on day 28 and 29. Diabetic mice with myocardial infarction showed an altered hemodynamic pattern and lipid profile, reduced injury markers, antioxidants with increased lipid peroxidation in the myocardium, and elevated glucose and liver enzymes in the blood. Moreover, an increased pro-inflammatory markers, histological severity, myonecrosis, and edema were observed. Curcumin compensated for hemodynamic fluctuations, restored biochemical markers, preserved antioxidant capacity, decreased cytokines levels, and restored cardiac functionality. However, the AM630 pre-treatment attenuated the effects of curcumin. The data suggest the involvement of CB2R in the actions of curcumin such as in the prevention of myocardial stress and in the improvement of the normal status of the myocardial membrane associated with diabetes. MDPI 2022-04-22 /pmc/articles/PMC9143027/ /pubmed/35629293 http://dx.doi.org/10.3390/life12050624 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pawar, Harshal D. Mahajan, Umesh B. Nakhate, Kartik T. Agrawal, Yogeeta O. Patil, Chandragouda R. Meeran, M. F. Nagoor Sharma, Charu Ojha, Shreesh Goyal, Sameer N. Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors |
title | Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors |
title_full | Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors |
title_fullStr | Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors |
title_full_unstemmed | Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors |
title_short | Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors |
title_sort | curcumin protects diabetic mice against isoproterenol-induced myocardial infarction by modulating cb2 cannabinoid receptors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143027/ https://www.ncbi.nlm.nih.gov/pubmed/35629293 http://dx.doi.org/10.3390/life12050624 |
work_keys_str_mv | AT pawarharshald curcuminprotectsdiabeticmiceagainstisoproterenolinducedmyocardialinfarctionbymodulatingcb2cannabinoidreceptors AT mahajanumeshb curcuminprotectsdiabeticmiceagainstisoproterenolinducedmyocardialinfarctionbymodulatingcb2cannabinoidreceptors AT nakhatekartikt curcuminprotectsdiabeticmiceagainstisoproterenolinducedmyocardialinfarctionbymodulatingcb2cannabinoidreceptors AT agrawalyogeetao curcuminprotectsdiabeticmiceagainstisoproterenolinducedmyocardialinfarctionbymodulatingcb2cannabinoidreceptors AT patilchandragoudar curcuminprotectsdiabeticmiceagainstisoproterenolinducedmyocardialinfarctionbymodulatingcb2cannabinoidreceptors AT meeranmfnagoor curcuminprotectsdiabeticmiceagainstisoproterenolinducedmyocardialinfarctionbymodulatingcb2cannabinoidreceptors AT sharmacharu curcuminprotectsdiabeticmiceagainstisoproterenolinducedmyocardialinfarctionbymodulatingcb2cannabinoidreceptors AT ojhashreesh curcuminprotectsdiabeticmiceagainstisoproterenolinducedmyocardialinfarctionbymodulatingcb2cannabinoidreceptors AT goyalsameern curcuminprotectsdiabeticmiceagainstisoproterenolinducedmyocardialinfarctionbymodulatingcb2cannabinoidreceptors |