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Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors

Molecular docking revealed curcumin as a potent CB2 cannabinoid receptor (CB2R) agonist. Since CB2R is involved in cardioprotective functions, we explored its role in ameliorative actions of curcumin against myocardial damage triggered by isoproterenol in diabetic animals. Mice were kept on a high-f...

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Autores principales: Pawar, Harshal D., Mahajan, Umesh B., Nakhate, Kartik T., Agrawal, Yogeeta O., Patil, Chandragouda R., Meeran, M. F. Nagoor, Sharma, Charu, Ojha, Shreesh, Goyal, Sameer N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143027/
https://www.ncbi.nlm.nih.gov/pubmed/35629293
http://dx.doi.org/10.3390/life12050624
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author Pawar, Harshal D.
Mahajan, Umesh B.
Nakhate, Kartik T.
Agrawal, Yogeeta O.
Patil, Chandragouda R.
Meeran, M. F. Nagoor
Sharma, Charu
Ojha, Shreesh
Goyal, Sameer N.
author_facet Pawar, Harshal D.
Mahajan, Umesh B.
Nakhate, Kartik T.
Agrawal, Yogeeta O.
Patil, Chandragouda R.
Meeran, M. F. Nagoor
Sharma, Charu
Ojha, Shreesh
Goyal, Sameer N.
author_sort Pawar, Harshal D.
collection PubMed
description Molecular docking revealed curcumin as a potent CB2 cannabinoid receptor (CB2R) agonist. Since CB2R is involved in cardioprotective functions, we explored its role in ameliorative actions of curcumin against myocardial damage triggered by isoproterenol in diabetic animals. Mice were kept on a high-fat diet (HFD) throughout the experiment (30 days). Following 7 days of HFD feeding, streptozotocin was administered (150 mg/kg, intraperitoneal) to induce diabetes. From day 11 to 30, diabetic mice received either curcumin (100 or 200 mg/kg/day, oral), CB2R antagonist AM630 (1 mg/kg/day, intraperitoneal) or both, with concurrent isoproterenol (150 mg/kg, subcutaneous) administration on day 28 and 29. Diabetic mice with myocardial infarction showed an altered hemodynamic pattern and lipid profile, reduced injury markers, antioxidants with increased lipid peroxidation in the myocardium, and elevated glucose and liver enzymes in the blood. Moreover, an increased pro-inflammatory markers, histological severity, myonecrosis, and edema were observed. Curcumin compensated for hemodynamic fluctuations, restored biochemical markers, preserved antioxidant capacity, decreased cytokines levels, and restored cardiac functionality. However, the AM630 pre-treatment attenuated the effects of curcumin. The data suggest the involvement of CB2R in the actions of curcumin such as in the prevention of myocardial stress and in the improvement of the normal status of the myocardial membrane associated with diabetes.
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spelling pubmed-91430272022-05-29 Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors Pawar, Harshal D. Mahajan, Umesh B. Nakhate, Kartik T. Agrawal, Yogeeta O. Patil, Chandragouda R. Meeran, M. F. Nagoor Sharma, Charu Ojha, Shreesh Goyal, Sameer N. Life (Basel) Article Molecular docking revealed curcumin as a potent CB2 cannabinoid receptor (CB2R) agonist. Since CB2R is involved in cardioprotective functions, we explored its role in ameliorative actions of curcumin against myocardial damage triggered by isoproterenol in diabetic animals. Mice were kept on a high-fat diet (HFD) throughout the experiment (30 days). Following 7 days of HFD feeding, streptozotocin was administered (150 mg/kg, intraperitoneal) to induce diabetes. From day 11 to 30, diabetic mice received either curcumin (100 or 200 mg/kg/day, oral), CB2R antagonist AM630 (1 mg/kg/day, intraperitoneal) or both, with concurrent isoproterenol (150 mg/kg, subcutaneous) administration on day 28 and 29. Diabetic mice with myocardial infarction showed an altered hemodynamic pattern and lipid profile, reduced injury markers, antioxidants with increased lipid peroxidation in the myocardium, and elevated glucose and liver enzymes in the blood. Moreover, an increased pro-inflammatory markers, histological severity, myonecrosis, and edema were observed. Curcumin compensated for hemodynamic fluctuations, restored biochemical markers, preserved antioxidant capacity, decreased cytokines levels, and restored cardiac functionality. However, the AM630 pre-treatment attenuated the effects of curcumin. The data suggest the involvement of CB2R in the actions of curcumin such as in the prevention of myocardial stress and in the improvement of the normal status of the myocardial membrane associated with diabetes. MDPI 2022-04-22 /pmc/articles/PMC9143027/ /pubmed/35629293 http://dx.doi.org/10.3390/life12050624 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pawar, Harshal D.
Mahajan, Umesh B.
Nakhate, Kartik T.
Agrawal, Yogeeta O.
Patil, Chandragouda R.
Meeran, M. F. Nagoor
Sharma, Charu
Ojha, Shreesh
Goyal, Sameer N.
Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors
title Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors
title_full Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors
title_fullStr Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors
title_full_unstemmed Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors
title_short Curcumin Protects Diabetic Mice against Isoproterenol-Induced Myocardial Infarction by Modulating CB2 Cannabinoid Receptors
title_sort curcumin protects diabetic mice against isoproterenol-induced myocardial infarction by modulating cb2 cannabinoid receptors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143027/
https://www.ncbi.nlm.nih.gov/pubmed/35629293
http://dx.doi.org/10.3390/life12050624
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