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Duplex One-Step RT-qPCR Assays for Simultaneous Detection of Genomic and Subgenomic RNAs of SARS-CoV-2 Variants

A hallmark of severe acute respiratory syndrome virus (SARS-CoV-2) replication is the discontinuous transcription of open reading frames (ORFs) encoding structural virus proteins. Real-time reverse transcription PCR (RT-qPCR) assays in previous publications used either single or multiplex assays for...

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Detalles Bibliográficos
Autores principales: Bhosle, Sushma M., Tran, Julie P., Yu, Shuiqing, Geiger, Jillian, Jackson, Jennifer D., Crozier, Ian, Crane, Anya, Wada, Jiro, Warren, Travis K., Kuhn, Jens H., Worwa, Gabriella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143037/
https://www.ncbi.nlm.nih.gov/pubmed/35632807
http://dx.doi.org/10.3390/v14051066