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Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine: Method Optimisation and Profiling in Healthy Adults upon Short-Term Dietary Intervention

S-adenosylmethionine (SAM) is essential for methyl transfer reactions. All SAM is produced de novo via the methionine cycle. The demethylation of SAM produces S-adenosylhomocysteine (SAH), an inhibitor of methyltransferases and the precursor of homocysteine (Hcy). The measurement of SAM and SAH in p...

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Autores principales: Bravo, Aida Corrillero, Aguilera, Maria Nieves Ligero, Marziali, Nahuel R., Moritz, Lennart, Wingert, Victoria, Klotz, Katharina, Schumann, Anke, Grünert, Sarah C., Spiekerkoetter, Ute, Berger, Urs, Lederer, Ann-Kathrin, Huber, Roman, Hannibal, Luciana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143066/
https://www.ncbi.nlm.nih.gov/pubmed/35629877
http://dx.doi.org/10.3390/metabo12050373
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author Bravo, Aida Corrillero
Aguilera, Maria Nieves Ligero
Marziali, Nahuel R.
Moritz, Lennart
Wingert, Victoria
Klotz, Katharina
Schumann, Anke
Grünert, Sarah C.
Spiekerkoetter, Ute
Berger, Urs
Lederer, Ann-Kathrin
Huber, Roman
Hannibal, Luciana
author_facet Bravo, Aida Corrillero
Aguilera, Maria Nieves Ligero
Marziali, Nahuel R.
Moritz, Lennart
Wingert, Victoria
Klotz, Katharina
Schumann, Anke
Grünert, Sarah C.
Spiekerkoetter, Ute
Berger, Urs
Lederer, Ann-Kathrin
Huber, Roman
Hannibal, Luciana
author_sort Bravo, Aida Corrillero
collection PubMed
description S-adenosylmethionine (SAM) is essential for methyl transfer reactions. All SAM is produced de novo via the methionine cycle. The demethylation of SAM produces S-adenosylhomocysteine (SAH), an inhibitor of methyltransferases and the precursor of homocysteine (Hcy). The measurement of SAM and SAH in plasma has value in the diagnosis of inborn errors of metabolism (IEM) and in research to assess methyl group homeostasis. The determination of SAM and SAH is complicated by the instability of SAM under neutral and alkaline conditions and the naturally low concentration of both SAM and SAH in plasma (nM range). Herein, we describe an optimised LC-MS/MS method for the determination of SAM and SAH in plasma, urine, and cells. The method is based on isotopic dilution and employs 20 µL of plasma or urine, or 500,000 cells, and has an instrumental running time of 5 min. The reference ranges for plasma SAM and SAH in a cohort of 33 healthy individuals (age: 19–60 years old; mean ± 2 SD) were 120 ± 36 nM and 21.5 ± 6.5 nM, respectively, in accordance with independent studies and diagnostic determinations. The method detected abnormal concentrations of SAM and SAH in patients with inborn errors of methyl group metabolism. Plasma and urinary SAM and SAH concentrations were determined for the first time in a randomised controlled trial of 53 healthy adult omnivores (age: 18–60 years old), before and after a 4 week intervention with a vegan or meat-rich diet, and revealed preserved variations of both metabolites and the SAM/SAH index.
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spelling pubmed-91430662022-05-29 Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine: Method Optimisation and Profiling in Healthy Adults upon Short-Term Dietary Intervention Bravo, Aida Corrillero Aguilera, Maria Nieves Ligero Marziali, Nahuel R. Moritz, Lennart Wingert, Victoria Klotz, Katharina Schumann, Anke Grünert, Sarah C. Spiekerkoetter, Ute Berger, Urs Lederer, Ann-Kathrin Huber, Roman Hannibal, Luciana Metabolites Article S-adenosylmethionine (SAM) is essential for methyl transfer reactions. All SAM is produced de novo via the methionine cycle. The demethylation of SAM produces S-adenosylhomocysteine (SAH), an inhibitor of methyltransferases and the precursor of homocysteine (Hcy). The measurement of SAM and SAH in plasma has value in the diagnosis of inborn errors of metabolism (IEM) and in research to assess methyl group homeostasis. The determination of SAM and SAH is complicated by the instability of SAM under neutral and alkaline conditions and the naturally low concentration of both SAM and SAH in plasma (nM range). Herein, we describe an optimised LC-MS/MS method for the determination of SAM and SAH in plasma, urine, and cells. The method is based on isotopic dilution and employs 20 µL of plasma or urine, or 500,000 cells, and has an instrumental running time of 5 min. The reference ranges for plasma SAM and SAH in a cohort of 33 healthy individuals (age: 19–60 years old; mean ± 2 SD) were 120 ± 36 nM and 21.5 ± 6.5 nM, respectively, in accordance with independent studies and diagnostic determinations. The method detected abnormal concentrations of SAM and SAH in patients with inborn errors of methyl group metabolism. Plasma and urinary SAM and SAH concentrations were determined for the first time in a randomised controlled trial of 53 healthy adult omnivores (age: 18–60 years old), before and after a 4 week intervention with a vegan or meat-rich diet, and revealed preserved variations of both metabolites and the SAM/SAH index. MDPI 2022-04-20 /pmc/articles/PMC9143066/ /pubmed/35629877 http://dx.doi.org/10.3390/metabo12050373 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bravo, Aida Corrillero
Aguilera, Maria Nieves Ligero
Marziali, Nahuel R.
Moritz, Lennart
Wingert, Victoria
Klotz, Katharina
Schumann, Anke
Grünert, Sarah C.
Spiekerkoetter, Ute
Berger, Urs
Lederer, Ann-Kathrin
Huber, Roman
Hannibal, Luciana
Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine: Method Optimisation and Profiling in Healthy Adults upon Short-Term Dietary Intervention
title Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine: Method Optimisation and Profiling in Healthy Adults upon Short-Term Dietary Intervention
title_full Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine: Method Optimisation and Profiling in Healthy Adults upon Short-Term Dietary Intervention
title_fullStr Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine: Method Optimisation and Profiling in Healthy Adults upon Short-Term Dietary Intervention
title_full_unstemmed Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine: Method Optimisation and Profiling in Healthy Adults upon Short-Term Dietary Intervention
title_short Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine: Method Optimisation and Profiling in Healthy Adults upon Short-Term Dietary Intervention
title_sort analysis of s-adenosylmethionine and s-adenosylhomocysteine: method optimisation and profiling in healthy adults upon short-term dietary intervention
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143066/
https://www.ncbi.nlm.nih.gov/pubmed/35629877
http://dx.doi.org/10.3390/metabo12050373
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