Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance

Here, we report the characterization of 13 novel caprine/ovine enterovirus strains isolated from different regions in China during 2016–2021. Immunoperoxidase monolayer assay showed that these viral strains shared strong cross-reaction with the previously reported caprine enterovirus CEV-JL14. Align...

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Autores principales: Chang, Xiaoran, Lin, Qian, Zhang, Qun, Hu, Junying, Tursun, Gulbahar, Deng, Yingrui, Guo, Chunguang, Wang, Xinping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143109/
https://www.ncbi.nlm.nih.gov/pubmed/35632794
http://dx.doi.org/10.3390/v14051051
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author Chang, Xiaoran
Lin, Qian
Zhang, Qun
Hu, Junying
Tursun, Gulbahar
Deng, Yingrui
Guo, Chunguang
Wang, Xinping
author_facet Chang, Xiaoran
Lin, Qian
Zhang, Qun
Hu, Junying
Tursun, Gulbahar
Deng, Yingrui
Guo, Chunguang
Wang, Xinping
author_sort Chang, Xiaoran
collection PubMed
description Here, we report the characterization of 13 novel caprine/ovine enterovirus strains isolated from different regions in China during 2016–2021. Immunoperoxidase monolayer assay showed that these viral strains shared strong cross-reaction with the previously reported caprine enterovirus CEV-JL14. Alignment analysis of the complete nucleotide sequences revealed 79.2%–87.8% and 75.0%–76.7% sequence identity of these novel caprine enterovirus strains to CEV-JL14 and TB4-OEV, respectively. Phylogenetic analyses clustered these novel strains to EV-G based on the amino acid sequences of P1 and 2C+3CD. Moreover, phylogenetic analysis of these caprine enterovirus strains identified three new EV-G types using VP1 sequences. These results demonstrate the genetic variations and the evolution of caprine enterovirus.
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spelling pubmed-91431092022-05-29 Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance Chang, Xiaoran Lin, Qian Zhang, Qun Hu, Junying Tursun, Gulbahar Deng, Yingrui Guo, Chunguang Wang, Xinping Viruses Article Here, we report the characterization of 13 novel caprine/ovine enterovirus strains isolated from different regions in China during 2016–2021. Immunoperoxidase monolayer assay showed that these viral strains shared strong cross-reaction with the previously reported caprine enterovirus CEV-JL14. Alignment analysis of the complete nucleotide sequences revealed 79.2%–87.8% and 75.0%–76.7% sequence identity of these novel caprine enterovirus strains to CEV-JL14 and TB4-OEV, respectively. Phylogenetic analyses clustered these novel strains to EV-G based on the amino acid sequences of P1 and 2C+3CD. Moreover, phylogenetic analysis of these caprine enterovirus strains identified three new EV-G types using VP1 sequences. These results demonstrate the genetic variations and the evolution of caprine enterovirus. MDPI 2022-05-15 /pmc/articles/PMC9143109/ /pubmed/35632794 http://dx.doi.org/10.3390/v14051051 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chang, Xiaoran
Lin, Qian
Zhang, Qun
Hu, Junying
Tursun, Gulbahar
Deng, Yingrui
Guo, Chunguang
Wang, Xinping
Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
title Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
title_full Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
title_fullStr Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
title_full_unstemmed Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
title_short Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
title_sort molecular analysis of caprine enterovirus circulating in china during 2016–2021: evolutionary significance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143109/
https://www.ncbi.nlm.nih.gov/pubmed/35632794
http://dx.doi.org/10.3390/v14051051
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