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An Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Salinispora Species

Cytochrome P450 monooxygenases (CYPs/P450s) are heme thiolate proteins present in species across the biological kingdoms. By virtue of their broad substrate promiscuity and regio- and stereo-selectivity, these enzymes enhance or attribute diversity to secondary metabolites. Actinomycetes species are...

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Autores principales: Malinga, Nsikelelo Allison, Nzuza, Nomfundo, Padayachee, Tiara, Syed, Puleng Rosinah, Karpoormath, Rajshekhar, Gront, Dominik, Nelson, David R., Syed, Khajamohiddin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143469/
https://www.ncbi.nlm.nih.gov/pubmed/35630316
http://dx.doi.org/10.3390/microorganisms10050871
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author Malinga, Nsikelelo Allison
Nzuza, Nomfundo
Padayachee, Tiara
Syed, Puleng Rosinah
Karpoormath, Rajshekhar
Gront, Dominik
Nelson, David R.
Syed, Khajamohiddin
author_facet Malinga, Nsikelelo Allison
Nzuza, Nomfundo
Padayachee, Tiara
Syed, Puleng Rosinah
Karpoormath, Rajshekhar
Gront, Dominik
Nelson, David R.
Syed, Khajamohiddin
author_sort Malinga, Nsikelelo Allison
collection PubMed
description Cytochrome P450 monooxygenases (CYPs/P450s) are heme thiolate proteins present in species across the biological kingdoms. By virtue of their broad substrate promiscuity and regio- and stereo-selectivity, these enzymes enhance or attribute diversity to secondary metabolites. Actinomycetes species are well-known producers of secondary metabolites, especially Salinispora species. Despite the importance of P450s, a comprehensive comparative analysis of P450s and their role in secondary metabolism in Salinispora species is not reported. We therefore analyzed P450s in 126 strains from three different species Salinispora arenicola, S. pacifica, and S. tropica. The study revealed the presence of 2643 P450s that can be grouped into 45 families and 103 subfamilies. CYP107 and CYP125 families are conserved, and CYP105 and CYP107 families are bloomed (a P450 family with many members) across Salinispora species. Analysis of P450s that are part of secondary metabolite biosynthetic gene clusters (smBGCs) revealed Salinispora species have an unprecedented number of P450s (1236 P450s-47%) part of smBGCs compared to other bacterial species belonging to the genera Streptomyces (23%) and Mycobacterium (11%), phyla Cyanobacteria (8%) and Firmicutes (18%) and the classes Alphaproteobacteria (2%) and Gammaproteobacteria (18%). A peculiar characteristic of up to six P450s in smBGCs was observed in Salinispora species. Future characterization Salinispora species P450s and their smBGCs have the potential for discovering novel secondary metabolites.
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spelling pubmed-91434692022-05-29 An Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Salinispora Species Malinga, Nsikelelo Allison Nzuza, Nomfundo Padayachee, Tiara Syed, Puleng Rosinah Karpoormath, Rajshekhar Gront, Dominik Nelson, David R. Syed, Khajamohiddin Microorganisms Article Cytochrome P450 monooxygenases (CYPs/P450s) are heme thiolate proteins present in species across the biological kingdoms. By virtue of their broad substrate promiscuity and regio- and stereo-selectivity, these enzymes enhance or attribute diversity to secondary metabolites. Actinomycetes species are well-known producers of secondary metabolites, especially Salinispora species. Despite the importance of P450s, a comprehensive comparative analysis of P450s and their role in secondary metabolism in Salinispora species is not reported. We therefore analyzed P450s in 126 strains from three different species Salinispora arenicola, S. pacifica, and S. tropica. The study revealed the presence of 2643 P450s that can be grouped into 45 families and 103 subfamilies. CYP107 and CYP125 families are conserved, and CYP105 and CYP107 families are bloomed (a P450 family with many members) across Salinispora species. Analysis of P450s that are part of secondary metabolite biosynthetic gene clusters (smBGCs) revealed Salinispora species have an unprecedented number of P450s (1236 P450s-47%) part of smBGCs compared to other bacterial species belonging to the genera Streptomyces (23%) and Mycobacterium (11%), phyla Cyanobacteria (8%) and Firmicutes (18%) and the classes Alphaproteobacteria (2%) and Gammaproteobacteria (18%). A peculiar characteristic of up to six P450s in smBGCs was observed in Salinispora species. Future characterization Salinispora species P450s and their smBGCs have the potential for discovering novel secondary metabolites. MDPI 2022-04-21 /pmc/articles/PMC9143469/ /pubmed/35630316 http://dx.doi.org/10.3390/microorganisms10050871 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Malinga, Nsikelelo Allison
Nzuza, Nomfundo
Padayachee, Tiara
Syed, Puleng Rosinah
Karpoormath, Rajshekhar
Gront, Dominik
Nelson, David R.
Syed, Khajamohiddin
An Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Salinispora Species
title An Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Salinispora Species
title_full An Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Salinispora Species
title_fullStr An Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Salinispora Species
title_full_unstemmed An Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Salinispora Species
title_short An Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Salinispora Species
title_sort unprecedented number of cytochrome p450s are involved in secondary metabolism in salinispora species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143469/
https://www.ncbi.nlm.nih.gov/pubmed/35630316
http://dx.doi.org/10.3390/microorganisms10050871
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