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The R2R3 MYB Transcription Factor MYB71 Regulates Abscisic Acid Response in Arabidopsis

Abscisic acid (ABA) regulates plant responses to abiotic stresses via regulating the expression of downstream genes, yet the functions of many ABA responsive genes remain unknown. We report here the characterization of MYB71, a R2R3 MYB transcription factor in regulating ABA responses in Arabidopsis...

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Autores principales: Cheng, Yuxin, Ma, Yanxing, Zhang, Na, Lin, Rao, Yuan, Yuan, Tian, Hainan, Hussain, Saddam, Chen, Siyu, Yang, Wenting, Cai, Ling, Li, Yingying, Wang, Xiaoping, Wang, Tianya, Wang, Shucai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143609/
https://www.ncbi.nlm.nih.gov/pubmed/35631794
http://dx.doi.org/10.3390/plants11101369
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author Cheng, Yuxin
Ma, Yanxing
Zhang, Na
Lin, Rao
Yuan, Yuan
Tian, Hainan
Hussain, Saddam
Chen, Siyu
Yang, Wenting
Cai, Ling
Li, Yingying
Wang, Xiaoping
Wang, Tianya
Wang, Shucai
author_facet Cheng, Yuxin
Ma, Yanxing
Zhang, Na
Lin, Rao
Yuan, Yuan
Tian, Hainan
Hussain, Saddam
Chen, Siyu
Yang, Wenting
Cai, Ling
Li, Yingying
Wang, Xiaoping
Wang, Tianya
Wang, Shucai
author_sort Cheng, Yuxin
collection PubMed
description Abscisic acid (ABA) regulates plant responses to abiotic stresses via regulating the expression of downstream genes, yet the functions of many ABA responsive genes remain unknown. We report here the characterization of MYB71, a R2R3 MYB transcription factor in regulating ABA responses in Arabidopsis. RT-PCR results show that the expression level of MYB71 was increased in response to ABA treatment. Arabidopsis protoplasts transfection results show that MYB71 was specifically localized in nucleus and it activated the Gal4:GUS reporter gene when recruited to the Gal4 promoter by a fused DNA binding domain GD. Roles of MYB71 in regulating plant response to ABA were analyzed by generating Arabidopsis transgenic plants overexpression MYB71 and gene edited mutants of MYB71. The results show that ABA sensitivity was increased in the transgenic plants overexpression MYB71, but decreased in the MYB71 mutants. By using a DEX inducible system, we further identified genes are likely regulated by MYB71, and found that they are enriched in biological process to environmental stimuli including abiotic stresses, suggesting that MYB71 may regulate plant response to abiotic stresses. Taken together, our results suggest that MYB71 is an ABA responsive gene, and MYB71 functions as a transcription activator and it positively regulates ABA response in Arabidopsis.
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spelling pubmed-91436092022-05-29 The R2R3 MYB Transcription Factor MYB71 Regulates Abscisic Acid Response in Arabidopsis Cheng, Yuxin Ma, Yanxing Zhang, Na Lin, Rao Yuan, Yuan Tian, Hainan Hussain, Saddam Chen, Siyu Yang, Wenting Cai, Ling Li, Yingying Wang, Xiaoping Wang, Tianya Wang, Shucai Plants (Basel) Article Abscisic acid (ABA) regulates plant responses to abiotic stresses via regulating the expression of downstream genes, yet the functions of many ABA responsive genes remain unknown. We report here the characterization of MYB71, a R2R3 MYB transcription factor in regulating ABA responses in Arabidopsis. RT-PCR results show that the expression level of MYB71 was increased in response to ABA treatment. Arabidopsis protoplasts transfection results show that MYB71 was specifically localized in nucleus and it activated the Gal4:GUS reporter gene when recruited to the Gal4 promoter by a fused DNA binding domain GD. Roles of MYB71 in regulating plant response to ABA were analyzed by generating Arabidopsis transgenic plants overexpression MYB71 and gene edited mutants of MYB71. The results show that ABA sensitivity was increased in the transgenic plants overexpression MYB71, but decreased in the MYB71 mutants. By using a DEX inducible system, we further identified genes are likely regulated by MYB71, and found that they are enriched in biological process to environmental stimuli including abiotic stresses, suggesting that MYB71 may regulate plant response to abiotic stresses. Taken together, our results suggest that MYB71 is an ABA responsive gene, and MYB71 functions as a transcription activator and it positively regulates ABA response in Arabidopsis. MDPI 2022-05-21 /pmc/articles/PMC9143609/ /pubmed/35631794 http://dx.doi.org/10.3390/plants11101369 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cheng, Yuxin
Ma, Yanxing
Zhang, Na
Lin, Rao
Yuan, Yuan
Tian, Hainan
Hussain, Saddam
Chen, Siyu
Yang, Wenting
Cai, Ling
Li, Yingying
Wang, Xiaoping
Wang, Tianya
Wang, Shucai
The R2R3 MYB Transcription Factor MYB71 Regulates Abscisic Acid Response in Arabidopsis
title The R2R3 MYB Transcription Factor MYB71 Regulates Abscisic Acid Response in Arabidopsis
title_full The R2R3 MYB Transcription Factor MYB71 Regulates Abscisic Acid Response in Arabidopsis
title_fullStr The R2R3 MYB Transcription Factor MYB71 Regulates Abscisic Acid Response in Arabidopsis
title_full_unstemmed The R2R3 MYB Transcription Factor MYB71 Regulates Abscisic Acid Response in Arabidopsis
title_short The R2R3 MYB Transcription Factor MYB71 Regulates Abscisic Acid Response in Arabidopsis
title_sort r2r3 myb transcription factor myb71 regulates abscisic acid response in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143609/
https://www.ncbi.nlm.nih.gov/pubmed/35631794
http://dx.doi.org/10.3390/plants11101369
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