Mechanisms of Trichomes and Terpene Compounds in Indigenous and Commercial Thai Rice Varieties against Brown Planthopper

SIMPLE SUMMARY: Herbivorous insects and their host plants have a long history of co-evolution. Plants produce specialized morphological structures known as trichomes, which may be involved in the antibiosis and antixenosis traits of the host plant when interacting with insect herbivores. The host plant also produces chemicals that may act as a repellent or antifeedant to reduce infestation by herbivores. Several studies over the last few decades have revealed that trichomes perform an important role in plants’ defense against herbivores. However, little information is available on the physical and chemical defense of indigenous and commercial Thai rice varieties against major pests such as the brown planthopper (BPH). In this study, we found a negative relationship between the density of prickle trichomes and the BPH infestation level. The volatile organic compound (VOC) emission profiles from rice plants indicated that β-Sesquiphellandrene induced by BPH possibly repelled BPH. ABSTRACT: Plant trichomes generally act as a physical defense against herbivore attacks and are present in a variety of plants, including rice plants. This research examined the physical and chemical defenses of rice plants against the brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae). A total of 10 rice varieties were used in this study. An electron microscope was used to observe trichomes. Constitutive and induced volatile compound profiles were assessed using GC-MS analyses. The preference of BPH for volatiles from the 10 rice plants was tested using a two-choice arena olfactometer system. The density of prickle trichomes had a negative relationship with the BPH injury level. Without BPH infestation, the volatile of the most resistant rice variety (Rathu Heenati (RH)) was preferred by BPH than those of the other varieties, with the exception of Gled Plah Chawn. However, the relative BPH preference for volatiles from the RH variety decreased during BPH infestation. When rice plants were infested by BPH, the numbers of VOCs and these quantities decreased. In the RH variety, the emission of essentities found without BPH infestation ceased during infestation by BPH. During the BPH infestation, rice plants started to emit new VOCs that were not detected before the BPH infestation started. In conclusion, we discovered that rice plants defended against BPH by changing VOC components during BPH infestation and β-Sesquiphellandrene was likely the most effective component.