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High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis

Coloured wastewater from the textile industry is a very serious global problem. Among 16 different white-rot fungal isolates, Trametes hirsuta PW17-41 revealed high potential for decolourisation of mixed textile dyes (Navy EC-R, Ruby S3B and Super Black G) from real industrial wastewater samples. Th...

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Autores principales: Thampraphaphon, Bancha, Phosri, Cherdchai, Pisutpaisal, Nipon, Thamvithayakorn, Pisit, Chotelersak, Kruawan, Sarp, Sarper, Suwannasai, Nuttika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143691/
https://www.ncbi.nlm.nih.gov/pubmed/35630435
http://dx.doi.org/10.3390/microorganisms10050992
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author Thampraphaphon, Bancha
Phosri, Cherdchai
Pisutpaisal, Nipon
Thamvithayakorn, Pisit
Chotelersak, Kruawan
Sarp, Sarper
Suwannasai, Nuttika
author_facet Thampraphaphon, Bancha
Phosri, Cherdchai
Pisutpaisal, Nipon
Thamvithayakorn, Pisit
Chotelersak, Kruawan
Sarp, Sarper
Suwannasai, Nuttika
author_sort Thampraphaphon, Bancha
collection PubMed
description Coloured wastewater from the textile industry is a very serious global problem. Among 16 different white-rot fungal isolates, Trametes hirsuta PW17-41 revealed high potential for decolourisation of mixed textile dyes (Navy EC-R, Ruby S3B and Super Black G) from real industrial wastewater samples. The efficiency of dye decolourisation was evaluated using the American Dye Manufacturers’ Institute (ADMI) standard methodology. The suitable support for fungal mycelium immobilisation was nylon sponges. The optimal dye decolourisation (95.39%) was achieved by using palm sugar and ammonium nitrate as carbon and nitrogen sources, respectively. The initial pH was 5 and the agitation speed was 100 rpm at 30 °C. The ADMI values of textile dyes decreased from 2475 to 114 within two days, reducing the treatment time from seven days before optimisation. The major mechanism of dye decolourisation was biodegradation, which was confirmed by UV–visible and FTIR spectra. Manganese peroxidase (MnP) (4942 U L(−1)) was found to be the main enzyme during the decolourisation process at an initial dye concentration of 21,200 ADMI. The results indicated the strong potential of immobilised fungal cells to remove high concentrations of textile dyes from industrial wastewater and their potential ability to produce high MnP and laccase activities that can be used in further application.
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spelling pubmed-91436912022-05-29 High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis Thampraphaphon, Bancha Phosri, Cherdchai Pisutpaisal, Nipon Thamvithayakorn, Pisit Chotelersak, Kruawan Sarp, Sarper Suwannasai, Nuttika Microorganisms Article Coloured wastewater from the textile industry is a very serious global problem. Among 16 different white-rot fungal isolates, Trametes hirsuta PW17-41 revealed high potential for decolourisation of mixed textile dyes (Navy EC-R, Ruby S3B and Super Black G) from real industrial wastewater samples. The efficiency of dye decolourisation was evaluated using the American Dye Manufacturers’ Institute (ADMI) standard methodology. The suitable support for fungal mycelium immobilisation was nylon sponges. The optimal dye decolourisation (95.39%) was achieved by using palm sugar and ammonium nitrate as carbon and nitrogen sources, respectively. The initial pH was 5 and the agitation speed was 100 rpm at 30 °C. The ADMI values of textile dyes decreased from 2475 to 114 within two days, reducing the treatment time from seven days before optimisation. The major mechanism of dye decolourisation was biodegradation, which was confirmed by UV–visible and FTIR spectra. Manganese peroxidase (MnP) (4942 U L(−1)) was found to be the main enzyme during the decolourisation process at an initial dye concentration of 21,200 ADMI. The results indicated the strong potential of immobilised fungal cells to remove high concentrations of textile dyes from industrial wastewater and their potential ability to produce high MnP and laccase activities that can be used in further application. MDPI 2022-05-09 /pmc/articles/PMC9143691/ /pubmed/35630435 http://dx.doi.org/10.3390/microorganisms10050992 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thampraphaphon, Bancha
Phosri, Cherdchai
Pisutpaisal, Nipon
Thamvithayakorn, Pisit
Chotelersak, Kruawan
Sarp, Sarper
Suwannasai, Nuttika
High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis
title High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis
title_full High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis
title_fullStr High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis
title_full_unstemmed High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis
title_short High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis
title_sort high potential decolourisation of textile dyes from wastewater by manganese peroxidase production of newly immobilised trametes hirsuta pw17-41 and ftir analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143691/
https://www.ncbi.nlm.nih.gov/pubmed/35630435
http://dx.doi.org/10.3390/microorganisms10050992
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