Cargando…

(123)I-BMIPP, a Radiopharmaceutical for Myocardial Fatty Acid Metabolism Scintigraphy, Could Be Utilized in Bacterial Infection Imaging

In this study, we evaluated the use of 15-(4-(123)I-iodophenyl)-3(R,S)-methylpentadecanoic acid ((123)I-BMIPP) to visualize fatty acid metabolism in bacteria for bacterial infection imaging. We found that (123)I-BMIPP, which is used for fatty acid metabolism scintigraphy in Japan, accumulated marked...

Descripción completa

Detalles Bibliográficos
Autores principales: Muranaka, Yuka, Mizutani, Asuka, Kobayashi, Masato, Nakamoto, Koya, Matsue, Miki, Takagi, Fumika, Okazaki, Kenichi, Nishi, Kodai, Yamazaki, Kana, Nishii, Ryuichi, Shikano, Naoto, Okamoto, Shigefumi, Maki, Hideki, Kawai, Keiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143722/
https://www.ncbi.nlm.nih.gov/pubmed/35631596
http://dx.doi.org/10.3390/pharmaceutics14051008
_version_ 1784715875645915136
author Muranaka, Yuka
Mizutani, Asuka
Kobayashi, Masato
Nakamoto, Koya
Matsue, Miki
Takagi, Fumika
Okazaki, Kenichi
Nishi, Kodai
Yamazaki, Kana
Nishii, Ryuichi
Shikano, Naoto
Okamoto, Shigefumi
Maki, Hideki
Kawai, Keiichi
author_facet Muranaka, Yuka
Mizutani, Asuka
Kobayashi, Masato
Nakamoto, Koya
Matsue, Miki
Takagi, Fumika
Okazaki, Kenichi
Nishi, Kodai
Yamazaki, Kana
Nishii, Ryuichi
Shikano, Naoto
Okamoto, Shigefumi
Maki, Hideki
Kawai, Keiichi
author_sort Muranaka, Yuka
collection PubMed
description In this study, we evaluated the use of 15-(4-(123)I-iodophenyl)-3(R,S)-methylpentadecanoic acid ((123)I-BMIPP) to visualize fatty acid metabolism in bacteria for bacterial infection imaging. We found that (123)I-BMIPP, which is used for fatty acid metabolism scintigraphy in Japan, accumulated markedly in Escherichia coli EC-14 similar to (18)F-FDG, which has previously been studied for bacterial imaging. To elucidate the underlying mechanism, we evaluated changes in (123)I-BMIPP accumulation under low-temperature conditions and in the presence of a CD36 inhibitor. The uptake of (123)I-BMIPP by EC-14 was mediated via the CD36-like fatty-acid-transporting membrane protein and accumulated by fatty acid metabolism. In model mice infected with EC-14, the biological distribution and whole-body imaging were assessed using (123)I-BMIPP and (18)F-FDG. The (123)I-BMIPP biodistribution study showed that, 8 h after infection, the ratio of (123)I-BMIPP accumulated in infected muscle to that in control muscle was 1.31 at 60 min after (123)I-BMIPP injection. In whole-body imaging 1.5 h after (123)I-BMIPP administration and 9.5 h after infection, infected muscle exhibited a 1.33-times higher contrast than non-infected muscle. Thus, (123)I-BMIPP shows potential for visualizing fatty acid metabolism of bacteria for imaging bacterial infections.
format Online
Article
Text
id pubmed-9143722
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91437222022-05-29 (123)I-BMIPP, a Radiopharmaceutical for Myocardial Fatty Acid Metabolism Scintigraphy, Could Be Utilized in Bacterial Infection Imaging Muranaka, Yuka Mizutani, Asuka Kobayashi, Masato Nakamoto, Koya Matsue, Miki Takagi, Fumika Okazaki, Kenichi Nishi, Kodai Yamazaki, Kana Nishii, Ryuichi Shikano, Naoto Okamoto, Shigefumi Maki, Hideki Kawai, Keiichi Pharmaceutics Article In this study, we evaluated the use of 15-(4-(123)I-iodophenyl)-3(R,S)-methylpentadecanoic acid ((123)I-BMIPP) to visualize fatty acid metabolism in bacteria for bacterial infection imaging. We found that (123)I-BMIPP, which is used for fatty acid metabolism scintigraphy in Japan, accumulated markedly in Escherichia coli EC-14 similar to (18)F-FDG, which has previously been studied for bacterial imaging. To elucidate the underlying mechanism, we evaluated changes in (123)I-BMIPP accumulation under low-temperature conditions and in the presence of a CD36 inhibitor. The uptake of (123)I-BMIPP by EC-14 was mediated via the CD36-like fatty-acid-transporting membrane protein and accumulated by fatty acid metabolism. In model mice infected with EC-14, the biological distribution and whole-body imaging were assessed using (123)I-BMIPP and (18)F-FDG. The (123)I-BMIPP biodistribution study showed that, 8 h after infection, the ratio of (123)I-BMIPP accumulated in infected muscle to that in control muscle was 1.31 at 60 min after (123)I-BMIPP injection. In whole-body imaging 1.5 h after (123)I-BMIPP administration and 9.5 h after infection, infected muscle exhibited a 1.33-times higher contrast than non-infected muscle. Thus, (123)I-BMIPP shows potential for visualizing fatty acid metabolism of bacteria for imaging bacterial infections. MDPI 2022-05-07 /pmc/articles/PMC9143722/ /pubmed/35631596 http://dx.doi.org/10.3390/pharmaceutics14051008 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Muranaka, Yuka
Mizutani, Asuka
Kobayashi, Masato
Nakamoto, Koya
Matsue, Miki
Takagi, Fumika
Okazaki, Kenichi
Nishi, Kodai
Yamazaki, Kana
Nishii, Ryuichi
Shikano, Naoto
Okamoto, Shigefumi
Maki, Hideki
Kawai, Keiichi
(123)I-BMIPP, a Radiopharmaceutical for Myocardial Fatty Acid Metabolism Scintigraphy, Could Be Utilized in Bacterial Infection Imaging
title (123)I-BMIPP, a Radiopharmaceutical for Myocardial Fatty Acid Metabolism Scintigraphy, Could Be Utilized in Bacterial Infection Imaging
title_full (123)I-BMIPP, a Radiopharmaceutical for Myocardial Fatty Acid Metabolism Scintigraphy, Could Be Utilized in Bacterial Infection Imaging
title_fullStr (123)I-BMIPP, a Radiopharmaceutical for Myocardial Fatty Acid Metabolism Scintigraphy, Could Be Utilized in Bacterial Infection Imaging
title_full_unstemmed (123)I-BMIPP, a Radiopharmaceutical for Myocardial Fatty Acid Metabolism Scintigraphy, Could Be Utilized in Bacterial Infection Imaging
title_short (123)I-BMIPP, a Radiopharmaceutical for Myocardial Fatty Acid Metabolism Scintigraphy, Could Be Utilized in Bacterial Infection Imaging
title_sort (123)i-bmipp, a radiopharmaceutical for myocardial fatty acid metabolism scintigraphy, could be utilized in bacterial infection imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143722/
https://www.ncbi.nlm.nih.gov/pubmed/35631596
http://dx.doi.org/10.3390/pharmaceutics14051008
work_keys_str_mv AT muranakayuka 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT mizutaniasuka 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT kobayashimasato 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT nakamotokoya 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT matsuemiki 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT takagifumika 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT okazakikenichi 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT nishikodai 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT yamazakikana 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT nishiiryuichi 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT shikanonaoto 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT okamotoshigefumi 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT makihideki 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging
AT kawaikeiichi 123ibmipparadiopharmaceuticalformyocardialfattyacidmetabolismscintigraphycouldbeutilizedinbacterialinfectionimaging