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Identifying and Manipulating Giant Vesicles: Review of Recent Approaches
Giant vesicles (GVs) are closed bilayer membranes that primarily comprise amphiphiles with diameters of more than 1 μm. Compared with regular vesicles (several tens of nanometers in size), GVs are of greater scientific interest as model cell membranes and protocells because of their structure and si...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144095/ https://www.ncbi.nlm.nih.gov/pubmed/35630111 http://dx.doi.org/10.3390/mi13050644 |
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author | Toyota, Taro Zhang, Yiting |
author_facet | Toyota, Taro Zhang, Yiting |
author_sort | Toyota, Taro |
collection | PubMed |
description | Giant vesicles (GVs) are closed bilayer membranes that primarily comprise amphiphiles with diameters of more than 1 μm. Compared with regular vesicles (several tens of nanometers in size), GVs are of greater scientific interest as model cell membranes and protocells because of their structure and size, which are similar to those of biological systems. Biopolymers and nano-/microparticles can be encapsulated in GVs at high concentrations, and their application as artificial cell bodies has piqued interest. It is essential to develop methods for investigating and manipulating the properties of GVs toward engineering applications. In this review, we discuss current improvements in microscopy, micromanipulation, and microfabrication technologies for progress in GV identification and engineering tools. Combined with the advancement of GV preparation technologies, these technological advancements can aid the development of artificial cell systems such as alternative tissues and GV-based chemical signal processing systems. |
format | Online Article Text |
id | pubmed-9144095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91440952022-05-29 Identifying and Manipulating Giant Vesicles: Review of Recent Approaches Toyota, Taro Zhang, Yiting Micromachines (Basel) Review Giant vesicles (GVs) are closed bilayer membranes that primarily comprise amphiphiles with diameters of more than 1 μm. Compared with regular vesicles (several tens of nanometers in size), GVs are of greater scientific interest as model cell membranes and protocells because of their structure and size, which are similar to those of biological systems. Biopolymers and nano-/microparticles can be encapsulated in GVs at high concentrations, and their application as artificial cell bodies has piqued interest. It is essential to develop methods for investigating and manipulating the properties of GVs toward engineering applications. In this review, we discuss current improvements in microscopy, micromanipulation, and microfabrication technologies for progress in GV identification and engineering tools. Combined with the advancement of GV preparation technologies, these technological advancements can aid the development of artificial cell systems such as alternative tissues and GV-based chemical signal processing systems. MDPI 2022-04-19 /pmc/articles/PMC9144095/ /pubmed/35630111 http://dx.doi.org/10.3390/mi13050644 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Toyota, Taro Zhang, Yiting Identifying and Manipulating Giant Vesicles: Review of Recent Approaches |
title | Identifying and Manipulating Giant Vesicles: Review of Recent Approaches |
title_full | Identifying and Manipulating Giant Vesicles: Review of Recent Approaches |
title_fullStr | Identifying and Manipulating Giant Vesicles: Review of Recent Approaches |
title_full_unstemmed | Identifying and Manipulating Giant Vesicles: Review of Recent Approaches |
title_short | Identifying and Manipulating Giant Vesicles: Review of Recent Approaches |
title_sort | identifying and manipulating giant vesicles: review of recent approaches |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144095/ https://www.ncbi.nlm.nih.gov/pubmed/35630111 http://dx.doi.org/10.3390/mi13050644 |
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