Cargando…

Fusarium Keratitis in Taiwan: Molecular Identification, Antifungal Susceptibilities, and Clinical Features

We performed molecular identification and antifungal susceptibilities of pathogens and investigated clinical features of 43 culture-proven Fusarium keratitis cases from 2015–2020 in Taiwan. The pathogens were identified by sequencing of their internal transcribed spacer regions of ribosomal DNA and...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Tsung-En, Ou, Jie-Hao, Hung, Ning, Yeh, Lung-Kun, Ma, David Hui-Kang, Tan, Hsin-Yuan, Chen, Hung-Chi, Hung, Kuo-Hsuan, Fan, Yun-Chen, Sun, Pei-Lun, Hsiao, Ching-Hsi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144221/
https://www.ncbi.nlm.nih.gov/pubmed/35628732
http://dx.doi.org/10.3390/jof8050476
_version_ 1784715996061237248
author Huang, Tsung-En
Ou, Jie-Hao
Hung, Ning
Yeh, Lung-Kun
Ma, David Hui-Kang
Tan, Hsin-Yuan
Chen, Hung-Chi
Hung, Kuo-Hsuan
Fan, Yun-Chen
Sun, Pei-Lun
Hsiao, Ching-Hsi
author_facet Huang, Tsung-En
Ou, Jie-Hao
Hung, Ning
Yeh, Lung-Kun
Ma, David Hui-Kang
Tan, Hsin-Yuan
Chen, Hung-Chi
Hung, Kuo-Hsuan
Fan, Yun-Chen
Sun, Pei-Lun
Hsiao, Ching-Hsi
author_sort Huang, Tsung-En
collection PubMed
description We performed molecular identification and antifungal susceptibilities of pathogens and investigated clinical features of 43 culture-proven Fusarium keratitis cases from 2015–2020 in Taiwan. The pathogens were identified by sequencing of their internal transcribed spacer regions of ribosomal DNA and translation elongation factor 1α gene; their antifungal susceptibilities (to seven agents) were determined by broth microdilution method. We also collected clinical data to compare the drug susceptibilities and clinical features of Fusarium solani species complex (FSSC) isolates with those of other Fusarium species complexes (non-FSSC). The FSSC accounted for 76.7% pathogens, among which F. falciforme (32.6%) and F. keratoplasticum (27.9%) were the most common species. Among clinically used antifungal agents, amphotericin B registered the lowest minimal inhibitory concentration (MIC), and the new azoles efinaconazole, lanoconazole and luliconazole, demonstrated even lower MICs against Fusarium species. The MICs of natamycin, voriconazole, chlorhexidine, lanoconazole, and luliconazole were higher for the FSSC than the non-FSSC, but no significant differences were noted in clinical outcomes, including corneal perforation and final visual acuity. In Taiwan, the FSSC was the most common complex in Fusarium keratitis; its MICs for five tested antifungal agents were higher than those of non-FSSC, but the clinical outcomes did not differ significantly.
format Online
Article
Text
id pubmed-9144221
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91442212022-05-29 Fusarium Keratitis in Taiwan: Molecular Identification, Antifungal Susceptibilities, and Clinical Features Huang, Tsung-En Ou, Jie-Hao Hung, Ning Yeh, Lung-Kun Ma, David Hui-Kang Tan, Hsin-Yuan Chen, Hung-Chi Hung, Kuo-Hsuan Fan, Yun-Chen Sun, Pei-Lun Hsiao, Ching-Hsi J Fungi (Basel) Article We performed molecular identification and antifungal susceptibilities of pathogens and investigated clinical features of 43 culture-proven Fusarium keratitis cases from 2015–2020 in Taiwan. The pathogens were identified by sequencing of their internal transcribed spacer regions of ribosomal DNA and translation elongation factor 1α gene; their antifungal susceptibilities (to seven agents) were determined by broth microdilution method. We also collected clinical data to compare the drug susceptibilities and clinical features of Fusarium solani species complex (FSSC) isolates with those of other Fusarium species complexes (non-FSSC). The FSSC accounted for 76.7% pathogens, among which F. falciforme (32.6%) and F. keratoplasticum (27.9%) were the most common species. Among clinically used antifungal agents, amphotericin B registered the lowest minimal inhibitory concentration (MIC), and the new azoles efinaconazole, lanoconazole and luliconazole, demonstrated even lower MICs against Fusarium species. The MICs of natamycin, voriconazole, chlorhexidine, lanoconazole, and luliconazole were higher for the FSSC than the non-FSSC, but no significant differences were noted in clinical outcomes, including corneal perforation and final visual acuity. In Taiwan, the FSSC was the most common complex in Fusarium keratitis; its MICs for five tested antifungal agents were higher than those of non-FSSC, but the clinical outcomes did not differ significantly. MDPI 2022-05-03 /pmc/articles/PMC9144221/ /pubmed/35628732 http://dx.doi.org/10.3390/jof8050476 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Tsung-En
Ou, Jie-Hao
Hung, Ning
Yeh, Lung-Kun
Ma, David Hui-Kang
Tan, Hsin-Yuan
Chen, Hung-Chi
Hung, Kuo-Hsuan
Fan, Yun-Chen
Sun, Pei-Lun
Hsiao, Ching-Hsi
Fusarium Keratitis in Taiwan: Molecular Identification, Antifungal Susceptibilities, and Clinical Features
title Fusarium Keratitis in Taiwan: Molecular Identification, Antifungal Susceptibilities, and Clinical Features
title_full Fusarium Keratitis in Taiwan: Molecular Identification, Antifungal Susceptibilities, and Clinical Features
title_fullStr Fusarium Keratitis in Taiwan: Molecular Identification, Antifungal Susceptibilities, and Clinical Features
title_full_unstemmed Fusarium Keratitis in Taiwan: Molecular Identification, Antifungal Susceptibilities, and Clinical Features
title_short Fusarium Keratitis in Taiwan: Molecular Identification, Antifungal Susceptibilities, and Clinical Features
title_sort fusarium keratitis in taiwan: molecular identification, antifungal susceptibilities, and clinical features
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144221/
https://www.ncbi.nlm.nih.gov/pubmed/35628732
http://dx.doi.org/10.3390/jof8050476
work_keys_str_mv AT huangtsungen fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures
AT oujiehao fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures
AT hungning fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures
AT yehlungkun fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures
AT madavidhuikang fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures
AT tanhsinyuan fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures
AT chenhungchi fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures
AT hungkuohsuan fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures
AT fanyunchen fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures
AT sunpeilun fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures
AT hsiaochinghsi fusariumkeratitisintaiwanmolecularidentificationantifungalsusceptibilitiesandclinicalfeatures