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Exposure of Mice to Thirdhand Smoke Modulates In Vitro and In Vivo Platelet Responses

Smoking is a risk factor for a variety of deleterious conditions, such as cancer, respiratory disease and cardiovascular disease. Thrombosis is an important and common aspect of several cardiovascular disease states, whose risk is known to be increased by both first- and secondhand smoke. More recen...

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Autores principales: Villalobos-García, Daniel, Ali, Hamdy E. A., Alarabi, Ahmed B., El-Halawany, Medhat S., Alshbool, Fatima Z., Khasawneh, Fadi T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144272/
https://www.ncbi.nlm.nih.gov/pubmed/35628405
http://dx.doi.org/10.3390/ijms23105595
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author Villalobos-García, Daniel
Ali, Hamdy E. A.
Alarabi, Ahmed B.
El-Halawany, Medhat S.
Alshbool, Fatima Z.
Khasawneh, Fadi T.
author_facet Villalobos-García, Daniel
Ali, Hamdy E. A.
Alarabi, Ahmed B.
El-Halawany, Medhat S.
Alshbool, Fatima Z.
Khasawneh, Fadi T.
author_sort Villalobos-García, Daniel
collection PubMed
description Smoking is a risk factor for a variety of deleterious conditions, such as cancer, respiratory disease and cardiovascular disease. Thrombosis is an important and common aspect of several cardiovascular disease states, whose risk is known to be increased by both first- and secondhand smoke. More recently, the residual cigarette smoke that persists after someone has smoked (referred to as thirdhand smoke or THS) has been gaining more attention, since it has been shown that it also negatively affects health. Indeed, we have previously shown that 6-month exposure to THS increases the risk of thrombogenesis. However, neither the time-dependence of THS-induced thrombus formation, nor its sex dependence have been investigated. Thus, in the present study, we investigated these issues in the context of a shorter exposure to THS, specifically 3 months, in male and female mice. We show that the platelets from 3-month THS-exposed mice exhibited enhanced activation by agonists. Moreover, we also show that mice of both sexes exposed to THS have decreased tail bleeding as well as decreased thrombus occlusion time. In terms of the role of sex, intersex disparities in thrombus development and hemostasis as well as in platelet aggregation were, interestingly, observed. Together, our findings show that exposing mice to THS for 3 months is sufficient to predispose them to thrombosis; which seems to be driven, at least in part, by an increased activity in platelets, and that it does not manifest equally in both sexes.
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spelling pubmed-91442722022-05-29 Exposure of Mice to Thirdhand Smoke Modulates In Vitro and In Vivo Platelet Responses Villalobos-García, Daniel Ali, Hamdy E. A. Alarabi, Ahmed B. El-Halawany, Medhat S. Alshbool, Fatima Z. Khasawneh, Fadi T. Int J Mol Sci Article Smoking is a risk factor for a variety of deleterious conditions, such as cancer, respiratory disease and cardiovascular disease. Thrombosis is an important and common aspect of several cardiovascular disease states, whose risk is known to be increased by both first- and secondhand smoke. More recently, the residual cigarette smoke that persists after someone has smoked (referred to as thirdhand smoke or THS) has been gaining more attention, since it has been shown that it also negatively affects health. Indeed, we have previously shown that 6-month exposure to THS increases the risk of thrombogenesis. However, neither the time-dependence of THS-induced thrombus formation, nor its sex dependence have been investigated. Thus, in the present study, we investigated these issues in the context of a shorter exposure to THS, specifically 3 months, in male and female mice. We show that the platelets from 3-month THS-exposed mice exhibited enhanced activation by agonists. Moreover, we also show that mice of both sexes exposed to THS have decreased tail bleeding as well as decreased thrombus occlusion time. In terms of the role of sex, intersex disparities in thrombus development and hemostasis as well as in platelet aggregation were, interestingly, observed. Together, our findings show that exposing mice to THS for 3 months is sufficient to predispose them to thrombosis; which seems to be driven, at least in part, by an increased activity in platelets, and that it does not manifest equally in both sexes. MDPI 2022-05-17 /pmc/articles/PMC9144272/ /pubmed/35628405 http://dx.doi.org/10.3390/ijms23105595 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Villalobos-García, Daniel
Ali, Hamdy E. A.
Alarabi, Ahmed B.
El-Halawany, Medhat S.
Alshbool, Fatima Z.
Khasawneh, Fadi T.
Exposure of Mice to Thirdhand Smoke Modulates In Vitro and In Vivo Platelet Responses
title Exposure of Mice to Thirdhand Smoke Modulates In Vitro and In Vivo Platelet Responses
title_full Exposure of Mice to Thirdhand Smoke Modulates In Vitro and In Vivo Platelet Responses
title_fullStr Exposure of Mice to Thirdhand Smoke Modulates In Vitro and In Vivo Platelet Responses
title_full_unstemmed Exposure of Mice to Thirdhand Smoke Modulates In Vitro and In Vivo Platelet Responses
title_short Exposure of Mice to Thirdhand Smoke Modulates In Vitro and In Vivo Platelet Responses
title_sort exposure of mice to thirdhand smoke modulates in vitro and in vivo platelet responses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144272/
https://www.ncbi.nlm.nih.gov/pubmed/35628405
http://dx.doi.org/10.3390/ijms23105595
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