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Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients

Background and Objectives: Periodontitis is a multifactorial inflammatory disease associated with biofilm dysbiosis and is defined by progressive periodontium destruction. Genes largely regulate this entire process. SIRTs are a group of histone deacetylases (HDACs) intimately involved in cell metabo...

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Autores principales: Kriaučiūnas, Albertas, Liutkevičienė, Rasa, Gedvilaitė, Greta, Kaikarytė, Kristė, Vilkevičiūtė, Alvita, Gleiznys, Darius, Pacauskienė, Ingrida, Žekonis, Gediminas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144289/
https://www.ncbi.nlm.nih.gov/pubmed/35630070
http://dx.doi.org/10.3390/medicina58050653
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author Kriaučiūnas, Albertas
Liutkevičienė, Rasa
Gedvilaitė, Greta
Kaikarytė, Kristė
Vilkevičiūtė, Alvita
Gleiznys, Darius
Pacauskienė, Ingrida
Žekonis, Gediminas
author_facet Kriaučiūnas, Albertas
Liutkevičienė, Rasa
Gedvilaitė, Greta
Kaikarytė, Kristė
Vilkevičiūtė, Alvita
Gleiznys, Darius
Pacauskienė, Ingrida
Žekonis, Gediminas
author_sort Kriaučiūnas, Albertas
collection PubMed
description Background and Objectives: Periodontitis is a multifactorial inflammatory disease associated with biofilm dysbiosis and is defined by progressive periodontium destruction. Genes largely regulate this entire process. SIRTs are a group of histone deacetylases (HDACs) intimately involved in cell metabolism and are responsible for altering and regulating numerous cell functions. Understanding SIRTs and their functions in periodontitis may be useful for therapeutic treatment strategies in the future. The aim of our study was to investigate the associations amid SIRT1 single-gene nucleotide polymorphisms (rs3818292, rs3758391, and rs7895833) and SIRT1 serum levels for patients affected by periodontitis in the Caucasian population. Materials and Methods: The study included 201 patients affected by periodontitis and 500 healthy controls. DNA extraction from peripheral leukocytes was carried out using commercial kits. The real-time PCR method was selected for the determination of the genotype of the periodontitis patients and the control group. The ELISA method was used to measure the SIRT1 concentration. A statistical data analysis was performed using “BM SPSS Statistics 27.0” software. Results: The SIRT1 rs3818292 AG genotype was associated with a 2-fold and 1.9-fold increase in the development of periodontitis under the codominant and overdominant models (OR = 1.959; CI = 1.239–3.098; p = 0.004; and OR = 1.944; CI = 1.230–3.073; p = 0.004, respectively). The serum SIRT1 levels were not statistically significantly different between subjects in the periodontitis and control groups (0.984 (5.159) ng/mL vs. 0.514 (7.705) ng/mL, p = 0.792). Conclusions: in our study, the genotypes and alleles of SIRT1 rs3818292, rs3758391, and rs7895833 statistically significantly differed between the periodontitis and control groups, exclusively in the male population and subjects older than 60 years.
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spelling pubmed-91442892022-05-29 Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients Kriaučiūnas, Albertas Liutkevičienė, Rasa Gedvilaitė, Greta Kaikarytė, Kristė Vilkevičiūtė, Alvita Gleiznys, Darius Pacauskienė, Ingrida Žekonis, Gediminas Medicina (Kaunas) Article Background and Objectives: Periodontitis is a multifactorial inflammatory disease associated with biofilm dysbiosis and is defined by progressive periodontium destruction. Genes largely regulate this entire process. SIRTs are a group of histone deacetylases (HDACs) intimately involved in cell metabolism and are responsible for altering and regulating numerous cell functions. Understanding SIRTs and their functions in periodontitis may be useful for therapeutic treatment strategies in the future. The aim of our study was to investigate the associations amid SIRT1 single-gene nucleotide polymorphisms (rs3818292, rs3758391, and rs7895833) and SIRT1 serum levels for patients affected by periodontitis in the Caucasian population. Materials and Methods: The study included 201 patients affected by periodontitis and 500 healthy controls. DNA extraction from peripheral leukocytes was carried out using commercial kits. The real-time PCR method was selected for the determination of the genotype of the periodontitis patients and the control group. The ELISA method was used to measure the SIRT1 concentration. A statistical data analysis was performed using “BM SPSS Statistics 27.0” software. Results: The SIRT1 rs3818292 AG genotype was associated with a 2-fold and 1.9-fold increase in the development of periodontitis under the codominant and overdominant models (OR = 1.959; CI = 1.239–3.098; p = 0.004; and OR = 1.944; CI = 1.230–3.073; p = 0.004, respectively). The serum SIRT1 levels were not statistically significantly different between subjects in the periodontitis and control groups (0.984 (5.159) ng/mL vs. 0.514 (7.705) ng/mL, p = 0.792). Conclusions: in our study, the genotypes and alleles of SIRT1 rs3818292, rs3758391, and rs7895833 statistically significantly differed between the periodontitis and control groups, exclusively in the male population and subjects older than 60 years. MDPI 2022-05-12 /pmc/articles/PMC9144289/ /pubmed/35630070 http://dx.doi.org/10.3390/medicina58050653 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kriaučiūnas, Albertas
Liutkevičienė, Rasa
Gedvilaitė, Greta
Kaikarytė, Kristė
Vilkevičiūtė, Alvita
Gleiznys, Darius
Pacauskienė, Ingrida
Žekonis, Gediminas
Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients
title Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients
title_full Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients
title_fullStr Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients
title_full_unstemmed Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients
title_short Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients
title_sort value of serum sirtuin-1 (sirt1) levels and sirt1 gene variants in periodontitis patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144289/
https://www.ncbi.nlm.nih.gov/pubmed/35630070
http://dx.doi.org/10.3390/medicina58050653
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