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Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins

An extensive understanding of the interactions between host cellular and viral proteins provides clues for studying novel antiviral strategies. Porcine circovirus type 3 (PCV3) and type 4 (PCV4) have recently been identified as viruses that can potentially damage the swine industry. Herein, 401 puta...

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Autores principales: Zhou, Jianwei, Wang, Yongxia, Zhou, Linyi, Qiu, Yonghui, Zhao, Jie, Dai, Beining, Feng, Xufei, Hou, Lei, Liu, Jue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144384/
https://www.ncbi.nlm.nih.gov/pubmed/35632681
http://dx.doi.org/10.3390/v14050939
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author Zhou, Jianwei
Wang, Yongxia
Zhou, Linyi
Qiu, Yonghui
Zhao, Jie
Dai, Beining
Feng, Xufei
Hou, Lei
Liu, Jue
author_facet Zhou, Jianwei
Wang, Yongxia
Zhou, Linyi
Qiu, Yonghui
Zhao, Jie
Dai, Beining
Feng, Xufei
Hou, Lei
Liu, Jue
author_sort Zhou, Jianwei
collection PubMed
description An extensive understanding of the interactions between host cellular and viral proteins provides clues for studying novel antiviral strategies. Porcine circovirus type 3 (PCV3) and type 4 (PCV4) have recently been identified as viruses that can potentially damage the swine industry. Herein, 401 putative PCV3 Cap-binding and 484 putative PCV4 Cap-binding proteins were characterized using co-immunoprecipitation and liquid chromatography-mass spectrometry. Both PCV3 and PCV4 Caps shared 278 identical interacting proteins, but some putative interacting proteins (123 for PCV3 Cap and 206 for PCV4 Cap) differed. A protein–protein interaction network was constructed, and according to gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analyses, both PCV3 Cap- and PCV4 Cap-binding proteins participated mainly in ribosome biogenesis, nucleic acid binding, and ATP-dependent RNA helicase activities. Verification assays of eight putative interacting proteins indicated that nucleophosmin-1, nucleolin, DEAD-box RNA helicase 21, heterogeneous nuclear ribonucleoprotein A2/B1, YTH N6-methyladenosine RNA binding protein 1, and Y-box binding protein 1 bound directly to both PCV3 and PCV4 Caps, but ring finger protein 2 and signal transducer and activator of transcription 6 did not. Therefore, the interaction network provided helpful information to support further research into the underlying mechanisms of PCV3 and PCV4 infection.
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spelling pubmed-91443842022-05-29 Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins Zhou, Jianwei Wang, Yongxia Zhou, Linyi Qiu, Yonghui Zhao, Jie Dai, Beining Feng, Xufei Hou, Lei Liu, Jue Viruses Article An extensive understanding of the interactions between host cellular and viral proteins provides clues for studying novel antiviral strategies. Porcine circovirus type 3 (PCV3) and type 4 (PCV4) have recently been identified as viruses that can potentially damage the swine industry. Herein, 401 putative PCV3 Cap-binding and 484 putative PCV4 Cap-binding proteins were characterized using co-immunoprecipitation and liquid chromatography-mass spectrometry. Both PCV3 and PCV4 Caps shared 278 identical interacting proteins, but some putative interacting proteins (123 for PCV3 Cap and 206 for PCV4 Cap) differed. A protein–protein interaction network was constructed, and according to gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analyses, both PCV3 Cap- and PCV4 Cap-binding proteins participated mainly in ribosome biogenesis, nucleic acid binding, and ATP-dependent RNA helicase activities. Verification assays of eight putative interacting proteins indicated that nucleophosmin-1, nucleolin, DEAD-box RNA helicase 21, heterogeneous nuclear ribonucleoprotein A2/B1, YTH N6-methyladenosine RNA binding protein 1, and Y-box binding protein 1 bound directly to both PCV3 and PCV4 Caps, but ring finger protein 2 and signal transducer and activator of transcription 6 did not. Therefore, the interaction network provided helpful information to support further research into the underlying mechanisms of PCV3 and PCV4 infection. MDPI 2022-04-29 /pmc/articles/PMC9144384/ /pubmed/35632681 http://dx.doi.org/10.3390/v14050939 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Jianwei
Wang, Yongxia
Zhou, Linyi
Qiu, Yonghui
Zhao, Jie
Dai, Beining
Feng, Xufei
Hou, Lei
Liu, Jue
Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
title Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
title_full Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
title_fullStr Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
title_full_unstemmed Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
title_short Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
title_sort interaction network of porcine circovirus type 3 and 4 capsids with host proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144384/
https://www.ncbi.nlm.nih.gov/pubmed/35632681
http://dx.doi.org/10.3390/v14050939
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