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Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits
Swine viral diseases challenge the sector’s sustainability by affecting productivity and the health and welfare of the animals. The lack of antiviral drugs and/or effective vaccines renders early and reliable diagnosis the basis of viral disease management, underlining the importance of point-of-car...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144544/ https://www.ncbi.nlm.nih.gov/pubmed/35632730 http://dx.doi.org/10.3390/v14050988 |
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author | Manessis, Georgios Frant, Maciej Wozniakowski, Grzegorz Nannucci, Lapo Benedetti, Martina Denes, Lilla Gyula, Balka Gelasakis, Athanasios I. Squires, Clare Recuero, Sara Sanchez, Carlos Griol, Amadeu Giusti, Alessandro Bossis, Ioannis |
author_facet | Manessis, Georgios Frant, Maciej Wozniakowski, Grzegorz Nannucci, Lapo Benedetti, Martina Denes, Lilla Gyula, Balka Gelasakis, Athanasios I. Squires, Clare Recuero, Sara Sanchez, Carlos Griol, Amadeu Giusti, Alessandro Bossis, Ioannis |
author_sort | Manessis, Georgios |
collection | PubMed |
description | Swine viral diseases challenge the sector’s sustainability by affecting productivity and the health and welfare of the animals. The lack of antiviral drugs and/or effective vaccines renders early and reliable diagnosis the basis of viral disease management, underlining the importance of point-of-care (POC) diagnostics. A novel POC diagnostic device utilizing photonic integrated circuits (PICs), microfluidics, and information and communication technologies for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A (SIV) was validated using spiked and clinical oral fluid samples. Metrics including sensitivity, specificity, accuracy, precision, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were calculated to assess the performance of the device. For PRRSV, the device achieved a sensitivity of 83.5%, specificity of 77.8%, and DOR values of 17.66, whereas the values for SIV were 81.8%, 82.2%, and 20.81, respectively. The POC device and PICs can be used for the detection of PRRSV and SIV in the field, paving the way for the introduction of novel technologies in the field of animal POC diagnostics to further optimize livestock biosecurity. |
format | Online Article Text |
id | pubmed-9144544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91445442022-05-29 Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits Manessis, Georgios Frant, Maciej Wozniakowski, Grzegorz Nannucci, Lapo Benedetti, Martina Denes, Lilla Gyula, Balka Gelasakis, Athanasios I. Squires, Clare Recuero, Sara Sanchez, Carlos Griol, Amadeu Giusti, Alessandro Bossis, Ioannis Viruses Article Swine viral diseases challenge the sector’s sustainability by affecting productivity and the health and welfare of the animals. The lack of antiviral drugs and/or effective vaccines renders early and reliable diagnosis the basis of viral disease management, underlining the importance of point-of-care (POC) diagnostics. A novel POC diagnostic device utilizing photonic integrated circuits (PICs), microfluidics, and information and communication technologies for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A (SIV) was validated using spiked and clinical oral fluid samples. Metrics including sensitivity, specificity, accuracy, precision, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were calculated to assess the performance of the device. For PRRSV, the device achieved a sensitivity of 83.5%, specificity of 77.8%, and DOR values of 17.66, whereas the values for SIV were 81.8%, 82.2%, and 20.81, respectively. The POC device and PICs can be used for the detection of PRRSV and SIV in the field, paving the way for the introduction of novel technologies in the field of animal POC diagnostics to further optimize livestock biosecurity. MDPI 2022-05-07 /pmc/articles/PMC9144544/ /pubmed/35632730 http://dx.doi.org/10.3390/v14050988 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Manessis, Georgios Frant, Maciej Wozniakowski, Grzegorz Nannucci, Lapo Benedetti, Martina Denes, Lilla Gyula, Balka Gelasakis, Athanasios I. Squires, Clare Recuero, Sara Sanchez, Carlos Griol, Amadeu Giusti, Alessandro Bossis, Ioannis Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits |
title | Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits |
title_full | Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits |
title_fullStr | Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits |
title_full_unstemmed | Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits |
title_short | Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits |
title_sort | point-of-care and label-free detection of porcine reproductive and respiratory syndrome and swine influenza viruses using a microfluidic device with photonic integrated circuits |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144544/ https://www.ncbi.nlm.nih.gov/pubmed/35632730 http://dx.doi.org/10.3390/v14050988 |
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