Cargando…

Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance

Enterovirus D68 (EVD68) was recently identified as an important cause of respiratory illness and acute flaccid myelitis (AFM), mostly in children. Here, we examined 472 pediatric patients diagnosed with severe respiratory illness and screened for EVD68 between April and October 2021. In parallel, sa...

Descripción completa

Detalles Bibliográficos
Autores principales: Erster, Oran, Bar-Or, Itay, Levy, Virginia, Shatzman-Steuerman, Rachel, Sofer, Danit, Weiss, Leah, Vasserman, Rinat, Fratty, Ilana S., Kestin, Klil, Elul, Michal, Levi, Nofar, Alkrenawi, Rola, Mendelson, Ella, Mandelboim, Michal, Weil, Merav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144596/
https://www.ncbi.nlm.nih.gov/pubmed/35632752
http://dx.doi.org/10.3390/v14051010
_version_ 1784716086815490048
author Erster, Oran
Bar-Or, Itay
Levy, Virginia
Shatzman-Steuerman, Rachel
Sofer, Danit
Weiss, Leah
Vasserman, Rinat
Fratty, Ilana S.
Kestin, Klil
Elul, Michal
Levi, Nofar
Alkrenawi, Rola
Mendelson, Ella
Mandelboim, Michal
Weil, Merav
author_facet Erster, Oran
Bar-Or, Itay
Levy, Virginia
Shatzman-Steuerman, Rachel
Sofer, Danit
Weiss, Leah
Vasserman, Rinat
Fratty, Ilana S.
Kestin, Klil
Elul, Michal
Levi, Nofar
Alkrenawi, Rola
Mendelson, Ella
Mandelboim, Michal
Weil, Merav
author_sort Erster, Oran
collection PubMed
description Enterovirus D68 (EVD68) was recently identified as an important cause of respiratory illness and acute flaccid myelitis (AFM), mostly in children. Here, we examined 472 pediatric patients diagnosed with severe respiratory illness and screened for EVD68 between April and October 2021. In parallel, samples collected from a wastewater treatment plant (WWTP) covering the residential area of the hospitalized patients were also tested for EVD68. Of the 472 clinical samples evaluated, 33 (7%) patients were positive for EVD68 RNA. All wastewater samples were positive for EVD68, with varying viral genome copy loads. Calculated EVD68 genome copies increased from the end of May until July 2021 and dramatically decreased at the beginning of August. A similar trend was observed in both clinical and wastewater samples during the period tested. Sequence analysis of EVD68-positive samples indicated that all samples originated from the same branch of subclade B3. This study is the first to use wastewater-based epidemiology (WBE) to monitor EVD68 dynamics by quantitative detection and shows a clear correlation with clinically diagnosed cases. These findings highlight the potential of WBE as an important tool for continuous surveillance of EVD68 and other enteroviruses.
format Online
Article
Text
id pubmed-9144596
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91445962022-05-29 Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance Erster, Oran Bar-Or, Itay Levy, Virginia Shatzman-Steuerman, Rachel Sofer, Danit Weiss, Leah Vasserman, Rinat Fratty, Ilana S. Kestin, Klil Elul, Michal Levi, Nofar Alkrenawi, Rola Mendelson, Ella Mandelboim, Michal Weil, Merav Viruses Article Enterovirus D68 (EVD68) was recently identified as an important cause of respiratory illness and acute flaccid myelitis (AFM), mostly in children. Here, we examined 472 pediatric patients diagnosed with severe respiratory illness and screened for EVD68 between April and October 2021. In parallel, samples collected from a wastewater treatment plant (WWTP) covering the residential area of the hospitalized patients were also tested for EVD68. Of the 472 clinical samples evaluated, 33 (7%) patients were positive for EVD68 RNA. All wastewater samples were positive for EVD68, with varying viral genome copy loads. Calculated EVD68 genome copies increased from the end of May until July 2021 and dramatically decreased at the beginning of August. A similar trend was observed in both clinical and wastewater samples during the period tested. Sequence analysis of EVD68-positive samples indicated that all samples originated from the same branch of subclade B3. This study is the first to use wastewater-based epidemiology (WBE) to monitor EVD68 dynamics by quantitative detection and shows a clear correlation with clinically diagnosed cases. These findings highlight the potential of WBE as an important tool for continuous surveillance of EVD68 and other enteroviruses. MDPI 2022-05-09 /pmc/articles/PMC9144596/ /pubmed/35632752 http://dx.doi.org/10.3390/v14051010 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Erster, Oran
Bar-Or, Itay
Levy, Virginia
Shatzman-Steuerman, Rachel
Sofer, Danit
Weiss, Leah
Vasserman, Rinat
Fratty, Ilana S.
Kestin, Klil
Elul, Michal
Levi, Nofar
Alkrenawi, Rola
Mendelson, Ella
Mandelboim, Michal
Weil, Merav
Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance
title Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance
title_full Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance
title_fullStr Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance
title_full_unstemmed Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance
title_short Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance
title_sort monitoring of enterovirus d68 outbreak in israel by a parallel clinical and wastewater based surveillance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144596/
https://www.ncbi.nlm.nih.gov/pubmed/35632752
http://dx.doi.org/10.3390/v14051010
work_keys_str_mv AT ersteroran monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT baroritay monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT levyvirginia monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT shatzmansteuermanrachel monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT soferdanit monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT weissleah monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT vassermanrinat monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT frattyilanas monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT kestinklil monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT elulmichal monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT levinofar monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT alkrenawirola monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT mendelsonella monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT mandelboimmichal monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance
AT weilmerav monitoringofenterovirusd68outbreakinisraelbyaparallelclinicalandwastewaterbasedsurveillance