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Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance
Enterovirus D68 (EVD68) was recently identified as an important cause of respiratory illness and acute flaccid myelitis (AFM), mostly in children. Here, we examined 472 pediatric patients diagnosed with severe respiratory illness and screened for EVD68 between April and October 2021. In parallel, sa...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144596/ https://www.ncbi.nlm.nih.gov/pubmed/35632752 http://dx.doi.org/10.3390/v14051010 |
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author | Erster, Oran Bar-Or, Itay Levy, Virginia Shatzman-Steuerman, Rachel Sofer, Danit Weiss, Leah Vasserman, Rinat Fratty, Ilana S. Kestin, Klil Elul, Michal Levi, Nofar Alkrenawi, Rola Mendelson, Ella Mandelboim, Michal Weil, Merav |
author_facet | Erster, Oran Bar-Or, Itay Levy, Virginia Shatzman-Steuerman, Rachel Sofer, Danit Weiss, Leah Vasserman, Rinat Fratty, Ilana S. Kestin, Klil Elul, Michal Levi, Nofar Alkrenawi, Rola Mendelson, Ella Mandelboim, Michal Weil, Merav |
author_sort | Erster, Oran |
collection | PubMed |
description | Enterovirus D68 (EVD68) was recently identified as an important cause of respiratory illness and acute flaccid myelitis (AFM), mostly in children. Here, we examined 472 pediatric patients diagnosed with severe respiratory illness and screened for EVD68 between April and October 2021. In parallel, samples collected from a wastewater treatment plant (WWTP) covering the residential area of the hospitalized patients were also tested for EVD68. Of the 472 clinical samples evaluated, 33 (7%) patients were positive for EVD68 RNA. All wastewater samples were positive for EVD68, with varying viral genome copy loads. Calculated EVD68 genome copies increased from the end of May until July 2021 and dramatically decreased at the beginning of August. A similar trend was observed in both clinical and wastewater samples during the period tested. Sequence analysis of EVD68-positive samples indicated that all samples originated from the same branch of subclade B3. This study is the first to use wastewater-based epidemiology (WBE) to monitor EVD68 dynamics by quantitative detection and shows a clear correlation with clinically diagnosed cases. These findings highlight the potential of WBE as an important tool for continuous surveillance of EVD68 and other enteroviruses. |
format | Online Article Text |
id | pubmed-9144596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91445962022-05-29 Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance Erster, Oran Bar-Or, Itay Levy, Virginia Shatzman-Steuerman, Rachel Sofer, Danit Weiss, Leah Vasserman, Rinat Fratty, Ilana S. Kestin, Klil Elul, Michal Levi, Nofar Alkrenawi, Rola Mendelson, Ella Mandelboim, Michal Weil, Merav Viruses Article Enterovirus D68 (EVD68) was recently identified as an important cause of respiratory illness and acute flaccid myelitis (AFM), mostly in children. Here, we examined 472 pediatric patients diagnosed with severe respiratory illness and screened for EVD68 between April and October 2021. In parallel, samples collected from a wastewater treatment plant (WWTP) covering the residential area of the hospitalized patients were also tested for EVD68. Of the 472 clinical samples evaluated, 33 (7%) patients were positive for EVD68 RNA. All wastewater samples were positive for EVD68, with varying viral genome copy loads. Calculated EVD68 genome copies increased from the end of May until July 2021 and dramatically decreased at the beginning of August. A similar trend was observed in both clinical and wastewater samples during the period tested. Sequence analysis of EVD68-positive samples indicated that all samples originated from the same branch of subclade B3. This study is the first to use wastewater-based epidemiology (WBE) to monitor EVD68 dynamics by quantitative detection and shows a clear correlation with clinically diagnosed cases. These findings highlight the potential of WBE as an important tool for continuous surveillance of EVD68 and other enteroviruses. MDPI 2022-05-09 /pmc/articles/PMC9144596/ /pubmed/35632752 http://dx.doi.org/10.3390/v14051010 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Erster, Oran Bar-Or, Itay Levy, Virginia Shatzman-Steuerman, Rachel Sofer, Danit Weiss, Leah Vasserman, Rinat Fratty, Ilana S. Kestin, Klil Elul, Michal Levi, Nofar Alkrenawi, Rola Mendelson, Ella Mandelboim, Michal Weil, Merav Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance |
title | Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance |
title_full | Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance |
title_fullStr | Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance |
title_full_unstemmed | Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance |
title_short | Monitoring of Enterovirus D68 Outbreak in Israel by a Parallel Clinical and Wastewater Based Surveillance |
title_sort | monitoring of enterovirus d68 outbreak in israel by a parallel clinical and wastewater based surveillance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144596/ https://www.ncbi.nlm.nih.gov/pubmed/35632752 http://dx.doi.org/10.3390/v14051010 |
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