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Towards 3D Bioprinted Spinal Cord Organoids
Three-dimensional (3D) cultures, so-called organoids, have emerged as an attractive tool for disease modeling and therapeutic innovations. Here, we aim to determine if boundary cap neural crest stem cells (BC) can survive and differentiate in gelatin-based 3D bioprinted bioink scaffolds in order to...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144715/ https://www.ncbi.nlm.nih.gov/pubmed/35628601 http://dx.doi.org/10.3390/ijms23105788 |
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author | Han, Yilin King, Marianne Tikhomirov, Evgenii Barasa, Povilas Souza, Cleide Dos Santos Lindh, Jonas Baltriukiene, Daiva Ferraiuolo, Laura Azzouz, Mimoun Gullo, Maurizio R. Kozlova, Elena N. |
author_facet | Han, Yilin King, Marianne Tikhomirov, Evgenii Barasa, Povilas Souza, Cleide Dos Santos Lindh, Jonas Baltriukiene, Daiva Ferraiuolo, Laura Azzouz, Mimoun Gullo, Maurizio R. Kozlova, Elena N. |
author_sort | Han, Yilin |
collection | PubMed |
description | Three-dimensional (3D) cultures, so-called organoids, have emerged as an attractive tool for disease modeling and therapeutic innovations. Here, we aim to determine if boundary cap neural crest stem cells (BC) can survive and differentiate in gelatin-based 3D bioprinted bioink scaffolds in order to establish an enabling technology for the fabrication of spinal cord organoids on a chip. BC previously demonstrated the ability to support survival and differentiation of co-implanted or co-cultured cells and supported motor neuron survival in excitotoxically challenged spinal cord slice cultures. We tested different combinations of bioink and cross-linked material, analyzed the survival of BC on the surface and inside the scaffolds, and then tested if human iPSC-derived neural cells (motor neuron precursors and astrocytes) can be printed with the same protocol, which was developed for BC. We showed that this protocol is applicable for human cells. Neural differentiation was more prominent in the peripheral compared to central parts of the printed construct, presumably because of easier access to differentiation-promoting factors in the medium. These findings show that the gelatin-based and enzymatically cross-linked hydrogel is a suitable bioink for building a multicellular, bioprinted spinal cord organoid, but that further measures are still required to achieve uniform neural differentiation. |
format | Online Article Text |
id | pubmed-9144715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91447152022-05-29 Towards 3D Bioprinted Spinal Cord Organoids Han, Yilin King, Marianne Tikhomirov, Evgenii Barasa, Povilas Souza, Cleide Dos Santos Lindh, Jonas Baltriukiene, Daiva Ferraiuolo, Laura Azzouz, Mimoun Gullo, Maurizio R. Kozlova, Elena N. Int J Mol Sci Article Three-dimensional (3D) cultures, so-called organoids, have emerged as an attractive tool for disease modeling and therapeutic innovations. Here, we aim to determine if boundary cap neural crest stem cells (BC) can survive and differentiate in gelatin-based 3D bioprinted bioink scaffolds in order to establish an enabling technology for the fabrication of spinal cord organoids on a chip. BC previously demonstrated the ability to support survival and differentiation of co-implanted or co-cultured cells and supported motor neuron survival in excitotoxically challenged spinal cord slice cultures. We tested different combinations of bioink and cross-linked material, analyzed the survival of BC on the surface and inside the scaffolds, and then tested if human iPSC-derived neural cells (motor neuron precursors and astrocytes) can be printed with the same protocol, which was developed for BC. We showed that this protocol is applicable for human cells. Neural differentiation was more prominent in the peripheral compared to central parts of the printed construct, presumably because of easier access to differentiation-promoting factors in the medium. These findings show that the gelatin-based and enzymatically cross-linked hydrogel is a suitable bioink for building a multicellular, bioprinted spinal cord organoid, but that further measures are still required to achieve uniform neural differentiation. MDPI 2022-05-21 /pmc/articles/PMC9144715/ /pubmed/35628601 http://dx.doi.org/10.3390/ijms23105788 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Han, Yilin King, Marianne Tikhomirov, Evgenii Barasa, Povilas Souza, Cleide Dos Santos Lindh, Jonas Baltriukiene, Daiva Ferraiuolo, Laura Azzouz, Mimoun Gullo, Maurizio R. Kozlova, Elena N. Towards 3D Bioprinted Spinal Cord Organoids |
title | Towards 3D Bioprinted Spinal Cord Organoids |
title_full | Towards 3D Bioprinted Spinal Cord Organoids |
title_fullStr | Towards 3D Bioprinted Spinal Cord Organoids |
title_full_unstemmed | Towards 3D Bioprinted Spinal Cord Organoids |
title_short | Towards 3D Bioprinted Spinal Cord Organoids |
title_sort | towards 3d bioprinted spinal cord organoids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144715/ https://www.ncbi.nlm.nih.gov/pubmed/35628601 http://dx.doi.org/10.3390/ijms23105788 |
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