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Towards 3D Bioprinted Spinal Cord Organoids

Three-dimensional (3D) cultures, so-called organoids, have emerged as an attractive tool for disease modeling and therapeutic innovations. Here, we aim to determine if boundary cap neural crest stem cells (BC) can survive and differentiate in gelatin-based 3D bioprinted bioink scaffolds in order to...

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Autores principales: Han, Yilin, King, Marianne, Tikhomirov, Evgenii, Barasa, Povilas, Souza, Cleide Dos Santos, Lindh, Jonas, Baltriukiene, Daiva, Ferraiuolo, Laura, Azzouz, Mimoun, Gullo, Maurizio R., Kozlova, Elena N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144715/
https://www.ncbi.nlm.nih.gov/pubmed/35628601
http://dx.doi.org/10.3390/ijms23105788
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author Han, Yilin
King, Marianne
Tikhomirov, Evgenii
Barasa, Povilas
Souza, Cleide Dos Santos
Lindh, Jonas
Baltriukiene, Daiva
Ferraiuolo, Laura
Azzouz, Mimoun
Gullo, Maurizio R.
Kozlova, Elena N.
author_facet Han, Yilin
King, Marianne
Tikhomirov, Evgenii
Barasa, Povilas
Souza, Cleide Dos Santos
Lindh, Jonas
Baltriukiene, Daiva
Ferraiuolo, Laura
Azzouz, Mimoun
Gullo, Maurizio R.
Kozlova, Elena N.
author_sort Han, Yilin
collection PubMed
description Three-dimensional (3D) cultures, so-called organoids, have emerged as an attractive tool for disease modeling and therapeutic innovations. Here, we aim to determine if boundary cap neural crest stem cells (BC) can survive and differentiate in gelatin-based 3D bioprinted bioink scaffolds in order to establish an enabling technology for the fabrication of spinal cord organoids on a chip. BC previously demonstrated the ability to support survival and differentiation of co-implanted or co-cultured cells and supported motor neuron survival in excitotoxically challenged spinal cord slice cultures. We tested different combinations of bioink and cross-linked material, analyzed the survival of BC on the surface and inside the scaffolds, and then tested if human iPSC-derived neural cells (motor neuron precursors and astrocytes) can be printed with the same protocol, which was developed for BC. We showed that this protocol is applicable for human cells. Neural differentiation was more prominent in the peripheral compared to central parts of the printed construct, presumably because of easier access to differentiation-promoting factors in the medium. These findings show that the gelatin-based and enzymatically cross-linked hydrogel is a suitable bioink for building a multicellular, bioprinted spinal cord organoid, but that further measures are still required to achieve uniform neural differentiation.
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spelling pubmed-91447152022-05-29 Towards 3D Bioprinted Spinal Cord Organoids Han, Yilin King, Marianne Tikhomirov, Evgenii Barasa, Povilas Souza, Cleide Dos Santos Lindh, Jonas Baltriukiene, Daiva Ferraiuolo, Laura Azzouz, Mimoun Gullo, Maurizio R. Kozlova, Elena N. Int J Mol Sci Article Three-dimensional (3D) cultures, so-called organoids, have emerged as an attractive tool for disease modeling and therapeutic innovations. Here, we aim to determine if boundary cap neural crest stem cells (BC) can survive and differentiate in gelatin-based 3D bioprinted bioink scaffolds in order to establish an enabling technology for the fabrication of spinal cord organoids on a chip. BC previously demonstrated the ability to support survival and differentiation of co-implanted or co-cultured cells and supported motor neuron survival in excitotoxically challenged spinal cord slice cultures. We tested different combinations of bioink and cross-linked material, analyzed the survival of BC on the surface and inside the scaffolds, and then tested if human iPSC-derived neural cells (motor neuron precursors and astrocytes) can be printed with the same protocol, which was developed for BC. We showed that this protocol is applicable for human cells. Neural differentiation was more prominent in the peripheral compared to central parts of the printed construct, presumably because of easier access to differentiation-promoting factors in the medium. These findings show that the gelatin-based and enzymatically cross-linked hydrogel is a suitable bioink for building a multicellular, bioprinted spinal cord organoid, but that further measures are still required to achieve uniform neural differentiation. MDPI 2022-05-21 /pmc/articles/PMC9144715/ /pubmed/35628601 http://dx.doi.org/10.3390/ijms23105788 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Yilin
King, Marianne
Tikhomirov, Evgenii
Barasa, Povilas
Souza, Cleide Dos Santos
Lindh, Jonas
Baltriukiene, Daiva
Ferraiuolo, Laura
Azzouz, Mimoun
Gullo, Maurizio R.
Kozlova, Elena N.
Towards 3D Bioprinted Spinal Cord Organoids
title Towards 3D Bioprinted Spinal Cord Organoids
title_full Towards 3D Bioprinted Spinal Cord Organoids
title_fullStr Towards 3D Bioprinted Spinal Cord Organoids
title_full_unstemmed Towards 3D Bioprinted Spinal Cord Organoids
title_short Towards 3D Bioprinted Spinal Cord Organoids
title_sort towards 3d bioprinted spinal cord organoids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144715/
https://www.ncbi.nlm.nih.gov/pubmed/35628601
http://dx.doi.org/10.3390/ijms23105788
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