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Production of 4-Deoxy-L-erythro-5-Hexoseulose Uronic Acid Using Two Free and Immobilized Alginate Lyases from Falsirhodobacter sp. Alg1

Falsirhodobacter sp. alg1 expresses two alginate lyases, AlyFRA and AlyFRB, to produce the linear monosaccharide 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) from alginate, metabolizing it to pyruvate. In this study, we prepared recombinant AlyFRA and AlyFRB and their immobilized enzymes and in...

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Autores principales: Tanaka, Yuzuki, Murase, Yoshihiro, Shibata, Toshiyuki, Tanaka, Reiji, Mori, Tetsushi, Miyake, Hideo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144776/
https://www.ncbi.nlm.nih.gov/pubmed/35630785
http://dx.doi.org/10.3390/molecules27103308
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author Tanaka, Yuzuki
Murase, Yoshihiro
Shibata, Toshiyuki
Tanaka, Reiji
Mori, Tetsushi
Miyake, Hideo
author_facet Tanaka, Yuzuki
Murase, Yoshihiro
Shibata, Toshiyuki
Tanaka, Reiji
Mori, Tetsushi
Miyake, Hideo
author_sort Tanaka, Yuzuki
collection PubMed
description Falsirhodobacter sp. alg1 expresses two alginate lyases, AlyFRA and AlyFRB, to produce the linear monosaccharide 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) from alginate, metabolizing it to pyruvate. In this study, we prepared recombinant AlyFRA and AlyFRB and their immobilized enzymes and investigated DEH production. Purified AlyFRA and AlyFRB reacted with sodium alginate and yielded approximately 96.8% DEH. Immobilized AlyFRA and AlyFRB were prepared using each crude enzyme solution and κ-carrageenan, and immobilized enzyme reuse in batch reactions and DEH yield were examined. Thus, DEH was produced in a relatively high yield of 79.6%, even after the immobilized enzyme was reused seven times. This method can produce DEH efficiently and at a low cost and can be used to mass produce the next generation of biofuels using brown algae.
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spelling pubmed-91447762022-05-29 Production of 4-Deoxy-L-erythro-5-Hexoseulose Uronic Acid Using Two Free and Immobilized Alginate Lyases from Falsirhodobacter sp. Alg1 Tanaka, Yuzuki Murase, Yoshihiro Shibata, Toshiyuki Tanaka, Reiji Mori, Tetsushi Miyake, Hideo Molecules Article Falsirhodobacter sp. alg1 expresses two alginate lyases, AlyFRA and AlyFRB, to produce the linear monosaccharide 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) from alginate, metabolizing it to pyruvate. In this study, we prepared recombinant AlyFRA and AlyFRB and their immobilized enzymes and investigated DEH production. Purified AlyFRA and AlyFRB reacted with sodium alginate and yielded approximately 96.8% DEH. Immobilized AlyFRA and AlyFRB were prepared using each crude enzyme solution and κ-carrageenan, and immobilized enzyme reuse in batch reactions and DEH yield were examined. Thus, DEH was produced in a relatively high yield of 79.6%, even after the immobilized enzyme was reused seven times. This method can produce DEH efficiently and at a low cost and can be used to mass produce the next generation of biofuels using brown algae. MDPI 2022-05-21 /pmc/articles/PMC9144776/ /pubmed/35630785 http://dx.doi.org/10.3390/molecules27103308 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tanaka, Yuzuki
Murase, Yoshihiro
Shibata, Toshiyuki
Tanaka, Reiji
Mori, Tetsushi
Miyake, Hideo
Production of 4-Deoxy-L-erythro-5-Hexoseulose Uronic Acid Using Two Free and Immobilized Alginate Lyases from Falsirhodobacter sp. Alg1
title Production of 4-Deoxy-L-erythro-5-Hexoseulose Uronic Acid Using Two Free and Immobilized Alginate Lyases from Falsirhodobacter sp. Alg1
title_full Production of 4-Deoxy-L-erythro-5-Hexoseulose Uronic Acid Using Two Free and Immobilized Alginate Lyases from Falsirhodobacter sp. Alg1
title_fullStr Production of 4-Deoxy-L-erythro-5-Hexoseulose Uronic Acid Using Two Free and Immobilized Alginate Lyases from Falsirhodobacter sp. Alg1
title_full_unstemmed Production of 4-Deoxy-L-erythro-5-Hexoseulose Uronic Acid Using Two Free and Immobilized Alginate Lyases from Falsirhodobacter sp. Alg1
title_short Production of 4-Deoxy-L-erythro-5-Hexoseulose Uronic Acid Using Two Free and Immobilized Alginate Lyases from Falsirhodobacter sp. Alg1
title_sort production of 4-deoxy-l-erythro-5-hexoseulose uronic acid using two free and immobilized alginate lyases from falsirhodobacter sp. alg1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144776/
https://www.ncbi.nlm.nih.gov/pubmed/35630785
http://dx.doi.org/10.3390/molecules27103308
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