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Cyanobacterial Root Associations of Leafless Epiphytic Orchids
The leafless orchids are rare epiphytic plants with extremely reduced leaves, and their aerial roots adopted for photosynthesis. The beneficial plant–microbial interactions contribute significantly to host nutrition, fitness, and growth. However, there are no data available on the bacterial associat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144888/ https://www.ncbi.nlm.nih.gov/pubmed/35630449 http://dx.doi.org/10.3390/microorganisms10051006 |
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author | Tsavkelova, Elena A. Glukhareva, Irina D. Volynchikova, Elena A. Egorova, Maria A. Leontieva, Maria R. Malakhova, Dina V. Kolomeitseva, Galina L. Netrusov, Alexander I. |
author_facet | Tsavkelova, Elena A. Glukhareva, Irina D. Volynchikova, Elena A. Egorova, Maria A. Leontieva, Maria R. Malakhova, Dina V. Kolomeitseva, Galina L. Netrusov, Alexander I. |
author_sort | Tsavkelova, Elena A. |
collection | PubMed |
description | The leafless orchids are rare epiphytic plants with extremely reduced leaves, and their aerial roots adopted for photosynthesis. The beneficial plant–microbial interactions contribute significantly to host nutrition, fitness, and growth. However, there are no data available on the bacterial associations, inhabiting leafless orchids. Here, we describe the diversity of cyanobacteria, which colonize the roots of greenhouse Microcoelia moreauae and Chiloschista parishii. The biodiversity and structure of the cyanobacterial community were analyzed using a complex approach, comprising traditional cultivable techniques, denaturing gradient gel electrophoresis (DGGE), and phylogenetic analysis, as well as the light and scanning electron microscopy (SEM). A wide diversity of associated bacteria colonize the root surface, forming massive biofilms on the aerial roots. The dominant populations of filamentous nitrogen-fixing cyanobacteria belonged to the orders Oscillatoriales, Synechococcales, and Nostocales. The composition of the cyanobacterial community varied, depending on the nitrogen supply. Two major groups prevailed under nitrogen-limiting conditions, belonging to Leptolyngbya sp. and Komarekiella sp. The latter was characterized by DGGE profiling and sequencing, as well as by its distinctive features of morphological plasticity. The leading role of these phototrophophic and diazotrophic cyanobacteria is discussed in terms of the epiphytic lifestyle of the leafless orchids. |
format | Online Article Text |
id | pubmed-9144888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91448882022-05-29 Cyanobacterial Root Associations of Leafless Epiphytic Orchids Tsavkelova, Elena A. Glukhareva, Irina D. Volynchikova, Elena A. Egorova, Maria A. Leontieva, Maria R. Malakhova, Dina V. Kolomeitseva, Galina L. Netrusov, Alexander I. Microorganisms Article The leafless orchids are rare epiphytic plants with extremely reduced leaves, and their aerial roots adopted for photosynthesis. The beneficial plant–microbial interactions contribute significantly to host nutrition, fitness, and growth. However, there are no data available on the bacterial associations, inhabiting leafless orchids. Here, we describe the diversity of cyanobacteria, which colonize the roots of greenhouse Microcoelia moreauae and Chiloschista parishii. The biodiversity and structure of the cyanobacterial community were analyzed using a complex approach, comprising traditional cultivable techniques, denaturing gradient gel electrophoresis (DGGE), and phylogenetic analysis, as well as the light and scanning electron microscopy (SEM). A wide diversity of associated bacteria colonize the root surface, forming massive biofilms on the aerial roots. The dominant populations of filamentous nitrogen-fixing cyanobacteria belonged to the orders Oscillatoriales, Synechococcales, and Nostocales. The composition of the cyanobacterial community varied, depending on the nitrogen supply. Two major groups prevailed under nitrogen-limiting conditions, belonging to Leptolyngbya sp. and Komarekiella sp. The latter was characterized by DGGE profiling and sequencing, as well as by its distinctive features of morphological plasticity. The leading role of these phototrophophic and diazotrophic cyanobacteria is discussed in terms of the epiphytic lifestyle of the leafless orchids. MDPI 2022-05-11 /pmc/articles/PMC9144888/ /pubmed/35630449 http://dx.doi.org/10.3390/microorganisms10051006 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tsavkelova, Elena A. Glukhareva, Irina D. Volynchikova, Elena A. Egorova, Maria A. Leontieva, Maria R. Malakhova, Dina V. Kolomeitseva, Galina L. Netrusov, Alexander I. Cyanobacterial Root Associations of Leafless Epiphytic Orchids |
title | Cyanobacterial Root Associations of Leafless Epiphytic Orchids |
title_full | Cyanobacterial Root Associations of Leafless Epiphytic Orchids |
title_fullStr | Cyanobacterial Root Associations of Leafless Epiphytic Orchids |
title_full_unstemmed | Cyanobacterial Root Associations of Leafless Epiphytic Orchids |
title_short | Cyanobacterial Root Associations of Leafless Epiphytic Orchids |
title_sort | cyanobacterial root associations of leafless epiphytic orchids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144888/ https://www.ncbi.nlm.nih.gov/pubmed/35630449 http://dx.doi.org/10.3390/microorganisms10051006 |
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