Cargando…

Socialization of Providencia stuartii Enables Resistance to Environmental Insults

Providencia stuartii is a highly social pathogen responsible for nosocomial chronic urinary tract infections. The bacterium indeed forms floating communities of cells (FCC) besides and prior-to canonical surface-attached biofilms (SAB). Within P. stuartii FCC, cells are riveted one to another owing...

Descripción completa

Detalles Bibliográficos
Autores principales: Lopes, Julie, Tetreau, Guillaume, Pounot, Kevin, El Khatib, Mariam, Colletier, Jacques-Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144925/
https://www.ncbi.nlm.nih.gov/pubmed/35630346
http://dx.doi.org/10.3390/microorganisms10050901
_version_ 1784716167193034752
author Lopes, Julie
Tetreau, Guillaume
Pounot, Kevin
El Khatib, Mariam
Colletier, Jacques-Philippe
author_facet Lopes, Julie
Tetreau, Guillaume
Pounot, Kevin
El Khatib, Mariam
Colletier, Jacques-Philippe
author_sort Lopes, Julie
collection PubMed
description Providencia stuartii is a highly social pathogen responsible for nosocomial chronic urinary tract infections. The bacterium indeed forms floating communities of cells (FCC) besides and prior-to canonical surface-attached biofilms (SAB). Within P. stuartii FCC, cells are riveted one to another owing to by self-interactions between its porins, viz. Omp-Pst1 and Omp-Pst2. In pathophysiological conditions, P. stuartii is principally exposed to high concentrations of urea, ammonia, bicarbonate, creatinine and to large variations of pH, questioning how these environmental cues affect socialization, and whether formation of SAB and FCC protects cells against those. Results from our investigations indicate that FCC and SAB can both form in the urinary tract, endowing cells with increased resistance and fitness. They additionally show that while Omp-Pst1 is the main gateway allowing penetration of urea, bicarbonate and ammonia into the periplasm, expression of Omp-Pst2 enables resistance to them.
format Online
Article
Text
id pubmed-9144925
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91449252022-05-29 Socialization of Providencia stuartii Enables Resistance to Environmental Insults Lopes, Julie Tetreau, Guillaume Pounot, Kevin El Khatib, Mariam Colletier, Jacques-Philippe Microorganisms Article Providencia stuartii is a highly social pathogen responsible for nosocomial chronic urinary tract infections. The bacterium indeed forms floating communities of cells (FCC) besides and prior-to canonical surface-attached biofilms (SAB). Within P. stuartii FCC, cells are riveted one to another owing to by self-interactions between its porins, viz. Omp-Pst1 and Omp-Pst2. In pathophysiological conditions, P. stuartii is principally exposed to high concentrations of urea, ammonia, bicarbonate, creatinine and to large variations of pH, questioning how these environmental cues affect socialization, and whether formation of SAB and FCC protects cells against those. Results from our investigations indicate that FCC and SAB can both form in the urinary tract, endowing cells with increased resistance and fitness. They additionally show that while Omp-Pst1 is the main gateway allowing penetration of urea, bicarbonate and ammonia into the periplasm, expression of Omp-Pst2 enables resistance to them. MDPI 2022-04-25 /pmc/articles/PMC9144925/ /pubmed/35630346 http://dx.doi.org/10.3390/microorganisms10050901 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lopes, Julie
Tetreau, Guillaume
Pounot, Kevin
El Khatib, Mariam
Colletier, Jacques-Philippe
Socialization of Providencia stuartii Enables Resistance to Environmental Insults
title Socialization of Providencia stuartii Enables Resistance to Environmental Insults
title_full Socialization of Providencia stuartii Enables Resistance to Environmental Insults
title_fullStr Socialization of Providencia stuartii Enables Resistance to Environmental Insults
title_full_unstemmed Socialization of Providencia stuartii Enables Resistance to Environmental Insults
title_short Socialization of Providencia stuartii Enables Resistance to Environmental Insults
title_sort socialization of providencia stuartii enables resistance to environmental insults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144925/
https://www.ncbi.nlm.nih.gov/pubmed/35630346
http://dx.doi.org/10.3390/microorganisms10050901
work_keys_str_mv AT lopesjulie socializationofprovidenciastuartiienablesresistancetoenvironmentalinsults
AT tetreauguillaume socializationofprovidenciastuartiienablesresistancetoenvironmentalinsults
AT pounotkevin socializationofprovidenciastuartiienablesresistancetoenvironmentalinsults
AT elkhatibmariam socializationofprovidenciastuartiienablesresistancetoenvironmentalinsults
AT colletierjacquesphilippe socializationofprovidenciastuartiienablesresistancetoenvironmentalinsults