Cargando…

Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression

Hydrodynamics play an important role in the rate of cell attachment and nutrient and oxygen transfer, which can affect biofilm development and the level of recombinant protein production. In the present study, the effects of different flow conditions on the development of Escherichia coli biofilms a...

Descripción completa

Detalles Bibliográficos
Autores principales: Soares, Alexandra, Gomes, Luciana C., Monteiro, Gabriel A., Mergulhão, Filipe J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145004/
https://www.ncbi.nlm.nih.gov/pubmed/35630375
http://dx.doi.org/10.3390/microorganisms10050931
_version_ 1784716186455375872
author Soares, Alexandra
Gomes, Luciana C.
Monteiro, Gabriel A.
Mergulhão, Filipe J.
author_facet Soares, Alexandra
Gomes, Luciana C.
Monteiro, Gabriel A.
Mergulhão, Filipe J.
author_sort Soares, Alexandra
collection PubMed
description Hydrodynamics play an important role in the rate of cell attachment and nutrient and oxygen transfer, which can affect biofilm development and the level of recombinant protein production. In the present study, the effects of different flow conditions on the development of Escherichia coli biofilms and the expression of a model recombinant protein (enhanced green fluorescent protein, eGFP) were examined. Planktonic and biofilm cells were grown at two different flow rates in a recirculating flow cell system for 7 days: 255 and 128 L h(−1) (corresponding to a Reynolds number of 4600 and 2300, respectively). The fluorometric analysis showed that the specific eGFP production was higher in biofilms than in planktonic cells under both hydrodynamic conditions (3-fold higher for 255 L h(−1) and 2-fold higher for 128 L h(−1)). In the biofilm cells, the percentage of eGFP-expressing cells was on average 52% higher at a flow rate of 255 L h(−1). Furthermore, a higher plasmid copy number (PCN) was obtained for the highest flow rate for both planktonic (244 PCN/cell versus 118 PCN/cell) and biofilm cells (43 PCN/cell versus 29 PCN/cell). The results suggested that higher flow velocities promoted eGFP expression in E. coli biofilms.
format Online
Article
Text
id pubmed-9145004
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91450042022-05-29 Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression Soares, Alexandra Gomes, Luciana C. Monteiro, Gabriel A. Mergulhão, Filipe J. Microorganisms Article Hydrodynamics play an important role in the rate of cell attachment and nutrient and oxygen transfer, which can affect biofilm development and the level of recombinant protein production. In the present study, the effects of different flow conditions on the development of Escherichia coli biofilms and the expression of a model recombinant protein (enhanced green fluorescent protein, eGFP) were examined. Planktonic and biofilm cells were grown at two different flow rates in a recirculating flow cell system for 7 days: 255 and 128 L h(−1) (corresponding to a Reynolds number of 4600 and 2300, respectively). The fluorometric analysis showed that the specific eGFP production was higher in biofilms than in planktonic cells under both hydrodynamic conditions (3-fold higher for 255 L h(−1) and 2-fold higher for 128 L h(−1)). In the biofilm cells, the percentage of eGFP-expressing cells was on average 52% higher at a flow rate of 255 L h(−1). Furthermore, a higher plasmid copy number (PCN) was obtained for the highest flow rate for both planktonic (244 PCN/cell versus 118 PCN/cell) and biofilm cells (43 PCN/cell versus 29 PCN/cell). The results suggested that higher flow velocities promoted eGFP expression in E. coli biofilms. MDPI 2022-04-29 /pmc/articles/PMC9145004/ /pubmed/35630375 http://dx.doi.org/10.3390/microorganisms10050931 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Soares, Alexandra
Gomes, Luciana C.
Monteiro, Gabriel A.
Mergulhão, Filipe J.
Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression
title Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression
title_full Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression
title_fullStr Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression
title_full_unstemmed Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression
title_short Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression
title_sort hydrodynamic effects on biofilm development and recombinant protein expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145004/
https://www.ncbi.nlm.nih.gov/pubmed/35630375
http://dx.doi.org/10.3390/microorganisms10050931
work_keys_str_mv AT soaresalexandra hydrodynamiceffectsonbiofilmdevelopmentandrecombinantproteinexpression
AT gomeslucianac hydrodynamiceffectsonbiofilmdevelopmentandrecombinantproteinexpression
AT monteirogabriela hydrodynamiceffectsonbiofilmdevelopmentandrecombinantproteinexpression
AT mergulhaofilipej hydrodynamiceffectsonbiofilmdevelopmentandrecombinantproteinexpression