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Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese

Rothia nasimurium was known previously as an opportunistic pathogen of animals. However, there are few reports regarding the pathogenicity of Rothia nasimurium. In September 2020, geese contracted a disease of unknown cause which brought economic losses to a farm in Jiangsu Province, China, promptin...

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Autores principales: Kang, Yuhui, Zhou, Hongshan, Jin, Wenjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145032/
https://www.ncbi.nlm.nih.gov/pubmed/35622725
http://dx.doi.org/10.3390/vetsci9050197
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author Kang, Yuhui
Zhou, Hongshan
Jin, Wenjie
author_facet Kang, Yuhui
Zhou, Hongshan
Jin, Wenjie
author_sort Kang, Yuhui
collection PubMed
description Rothia nasimurium was known previously as an opportunistic pathogen of animals. However, there are few reports regarding the pathogenicity of Rothia nasimurium. In September 2020, geese contracted a disease of unknown cause which brought economic losses to a farm in Jiangsu Province, China, prompting a series of investigations. The bacterium was isolated, cultured, and purified, and then identified using Gram staining, biochemical tests, matrix-assisted laser desorption/ionization time of flight mass spectrometry, and 16S rRNA sequence analysis. After determining the obtained bacteria species, antibiotic susceptibility tests and animal regression experiments were carried out. A strain of bacterium was successfully isolated from the livers of the diseased geese, which was identified as a strain of the Gram-positive bacterium Rothia nasimurium according to the 16S rRNA sequencing results. By indexing references, no goose was reported to have been infected with Rothia nasimurium. The antibiotic susceptibility testing showed that only four antibiotics (amikacin, cefazolin, fosfomycin, and ampicillin/sulbactam) could effectively inhibit the growth of the Rothia nasimurium strain. The animal regression experiments showed that the novel isolated strain could infect goslings, and it also causes serious depilation of goslings. The results of the manuscript expanded the range of pathogenic microorganisms in geese, which is helpful to develop methods for avian endemic control.
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spelling pubmed-91450322022-05-29 Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese Kang, Yuhui Zhou, Hongshan Jin, Wenjie Vet Sci Article Rothia nasimurium was known previously as an opportunistic pathogen of animals. However, there are few reports regarding the pathogenicity of Rothia nasimurium. In September 2020, geese contracted a disease of unknown cause which brought economic losses to a farm in Jiangsu Province, China, prompting a series of investigations. The bacterium was isolated, cultured, and purified, and then identified using Gram staining, biochemical tests, matrix-assisted laser desorption/ionization time of flight mass spectrometry, and 16S rRNA sequence analysis. After determining the obtained bacteria species, antibiotic susceptibility tests and animal regression experiments were carried out. A strain of bacterium was successfully isolated from the livers of the diseased geese, which was identified as a strain of the Gram-positive bacterium Rothia nasimurium according to the 16S rRNA sequencing results. By indexing references, no goose was reported to have been infected with Rothia nasimurium. The antibiotic susceptibility testing showed that only four antibiotics (amikacin, cefazolin, fosfomycin, and ampicillin/sulbactam) could effectively inhibit the growth of the Rothia nasimurium strain. The animal regression experiments showed that the novel isolated strain could infect goslings, and it also causes serious depilation of goslings. The results of the manuscript expanded the range of pathogenic microorganisms in geese, which is helpful to develop methods for avian endemic control. MDPI 2022-04-21 /pmc/articles/PMC9145032/ /pubmed/35622725 http://dx.doi.org/10.3390/vetsci9050197 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kang, Yuhui
Zhou, Hongshan
Jin, Wenjie
Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese
title Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese
title_full Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese
title_fullStr Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese
title_full_unstemmed Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese
title_short Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese
title_sort rothia nasimurium as a cause of disease: first isolation from farmed geese
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145032/
https://www.ncbi.nlm.nih.gov/pubmed/35622725
http://dx.doi.org/10.3390/vetsci9050197
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