Cargando…

Chemical Constituent Analysis of Ranunculus sceleratus L. Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Orbitrap High-Resolution Mass Spectrometry

Ranunculus sceleratus L.(RS) has shown various pharmacological effects in traditional Chinese medicine. In our previous study, the positive therapeutic effect on α-naphthylisothiocyanate induced intrahepatic cholestasis in rats was obtained using TianJiu treatment with fresh RS. However, the chemica...

Descripción completa

Detalles Bibliográficos
Autores principales: Cao, Shanshan, Hu, Min, Yang, Lingli, Li, Meiqin, Shi, Zhen, Cheng, Wenming, Zhang, Yazhong, Chen, Fei, Wang, Sheng, Zhang, Qunlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145087/
https://www.ncbi.nlm.nih.gov/pubmed/35630779
http://dx.doi.org/10.3390/molecules27103299
_version_ 1784716207243395072
author Cao, Shanshan
Hu, Min
Yang, Lingli
Li, Meiqin
Shi, Zhen
Cheng, Wenming
Zhang, Yazhong
Chen, Fei
Wang, Sheng
Zhang, Qunlin
author_facet Cao, Shanshan
Hu, Min
Yang, Lingli
Li, Meiqin
Shi, Zhen
Cheng, Wenming
Zhang, Yazhong
Chen, Fei
Wang, Sheng
Zhang, Qunlin
author_sort Cao, Shanshan
collection PubMed
description Ranunculus sceleratus L.(RS) has shown various pharmacological effects in traditional Chinese medicine. In our previous study, the positive therapeutic effect on α-naphthylisothiocyanate induced intrahepatic cholestasis in rats was obtained using TianJiu treatment with fresh RS. However, the chemical profile of RS has not been clearly clarified, which impedes the research progress on the therapeutic effect of RS. Herein, an ultra-high performance liquid chromatography coupled with quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) method was developed to rapidly separate and identify multiple constituents in the 80% methanol extract of RS. A total of sixty-nine compounds (19 flavonoids, 22 organic acids, 6 coumarins, 4 lignans, 14 nitrogenous compounds, and 4 anthraquinones) were successfully characterized. A total of 12 of these compounds were unambiguously identified by standard samples. Their mass spectrometric fragmentation pathways were investigated. It is worth noting that flavonoids and lignans were identified for the first time in RS. In this study, we successfully provide the first comprehensive report on identifying major chemical constituents in RS by UHPLC-Q-Orbitrap HRMS. The obtained results enrich the RS chemical profile, paving the way for further phytochemical study, quality control, and pharmacological investigation of RS.
format Online
Article
Text
id pubmed-9145087
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91450872022-05-29 Chemical Constituent Analysis of Ranunculus sceleratus L. Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Orbitrap High-Resolution Mass Spectrometry Cao, Shanshan Hu, Min Yang, Lingli Li, Meiqin Shi, Zhen Cheng, Wenming Zhang, Yazhong Chen, Fei Wang, Sheng Zhang, Qunlin Molecules Article Ranunculus sceleratus L.(RS) has shown various pharmacological effects in traditional Chinese medicine. In our previous study, the positive therapeutic effect on α-naphthylisothiocyanate induced intrahepatic cholestasis in rats was obtained using TianJiu treatment with fresh RS. However, the chemical profile of RS has not been clearly clarified, which impedes the research progress on the therapeutic effect of RS. Herein, an ultra-high performance liquid chromatography coupled with quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) method was developed to rapidly separate and identify multiple constituents in the 80% methanol extract of RS. A total of sixty-nine compounds (19 flavonoids, 22 organic acids, 6 coumarins, 4 lignans, 14 nitrogenous compounds, and 4 anthraquinones) were successfully characterized. A total of 12 of these compounds were unambiguously identified by standard samples. Their mass spectrometric fragmentation pathways were investigated. It is worth noting that flavonoids and lignans were identified for the first time in RS. In this study, we successfully provide the first comprehensive report on identifying major chemical constituents in RS by UHPLC-Q-Orbitrap HRMS. The obtained results enrich the RS chemical profile, paving the way for further phytochemical study, quality control, and pharmacological investigation of RS. MDPI 2022-05-20 /pmc/articles/PMC9145087/ /pubmed/35630779 http://dx.doi.org/10.3390/molecules27103299 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cao, Shanshan
Hu, Min
Yang, Lingli
Li, Meiqin
Shi, Zhen
Cheng, Wenming
Zhang, Yazhong
Chen, Fei
Wang, Sheng
Zhang, Qunlin
Chemical Constituent Analysis of Ranunculus sceleratus L. Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Orbitrap High-Resolution Mass Spectrometry
title Chemical Constituent Analysis of Ranunculus sceleratus L. Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Orbitrap High-Resolution Mass Spectrometry
title_full Chemical Constituent Analysis of Ranunculus sceleratus L. Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Orbitrap High-Resolution Mass Spectrometry
title_fullStr Chemical Constituent Analysis of Ranunculus sceleratus L. Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Orbitrap High-Resolution Mass Spectrometry
title_full_unstemmed Chemical Constituent Analysis of Ranunculus sceleratus L. Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Orbitrap High-Resolution Mass Spectrometry
title_short Chemical Constituent Analysis of Ranunculus sceleratus L. Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Orbitrap High-Resolution Mass Spectrometry
title_sort chemical constituent analysis of ranunculus sceleratus l. using ultra-high-performance liquid chromatography coupled with quadrupole-orbitrap high-resolution mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145087/
https://www.ncbi.nlm.nih.gov/pubmed/35630779
http://dx.doi.org/10.3390/molecules27103299
work_keys_str_mv AT caoshanshan chemicalconstituentanalysisofranunculussceleratuslusingultrahighperformanceliquidchromatographycoupledwithquadrupoleorbitraphighresolutionmassspectrometry
AT humin chemicalconstituentanalysisofranunculussceleratuslusingultrahighperformanceliquidchromatographycoupledwithquadrupoleorbitraphighresolutionmassspectrometry
AT yanglingli chemicalconstituentanalysisofranunculussceleratuslusingultrahighperformanceliquidchromatographycoupledwithquadrupoleorbitraphighresolutionmassspectrometry
AT limeiqin chemicalconstituentanalysisofranunculussceleratuslusingultrahighperformanceliquidchromatographycoupledwithquadrupoleorbitraphighresolutionmassspectrometry
AT shizhen chemicalconstituentanalysisofranunculussceleratuslusingultrahighperformanceliquidchromatographycoupledwithquadrupoleorbitraphighresolutionmassspectrometry
AT chengwenming chemicalconstituentanalysisofranunculussceleratuslusingultrahighperformanceliquidchromatographycoupledwithquadrupoleorbitraphighresolutionmassspectrometry
AT zhangyazhong chemicalconstituentanalysisofranunculussceleratuslusingultrahighperformanceliquidchromatographycoupledwithquadrupoleorbitraphighresolutionmassspectrometry
AT chenfei chemicalconstituentanalysisofranunculussceleratuslusingultrahighperformanceliquidchromatographycoupledwithquadrupoleorbitraphighresolutionmassspectrometry
AT wangsheng chemicalconstituentanalysisofranunculussceleratuslusingultrahighperformanceliquidchromatographycoupledwithquadrupoleorbitraphighresolutionmassspectrometry
AT zhangqunlin chemicalconstituentanalysisofranunculussceleratuslusingultrahighperformanceliquidchromatographycoupledwithquadrupoleorbitraphighresolutionmassspectrometry