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Combined Effects of Potassium Perchlorate and a Neonicotinoid on Zebrafish Larvae (Danio rerio)

Imidacloprid (IMI) is part of the neonicotinoids family, insecticides widely used by humans and also found in wastewater. This class of compounds, if present in the environment, can cause toxicity to different species such as bees and gammarids, although little is known about vertebrates such as fis...

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Autores principales: Paola, Davide Di, Capparucci, Fabiano, Natale, Sabrina, Crupi, Rosalia, Cuzzocrea, Salvatore, Spanò, Nunziacarla, Gugliandolo, Enrico, Peritore, Alessio Filippo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145203/
https://www.ncbi.nlm.nih.gov/pubmed/35622618
http://dx.doi.org/10.3390/toxics10050203
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author Paola, Davide Di
Capparucci, Fabiano
Natale, Sabrina
Crupi, Rosalia
Cuzzocrea, Salvatore
Spanò, Nunziacarla
Gugliandolo, Enrico
Peritore, Alessio Filippo
author_facet Paola, Davide Di
Capparucci, Fabiano
Natale, Sabrina
Crupi, Rosalia
Cuzzocrea, Salvatore
Spanò, Nunziacarla
Gugliandolo, Enrico
Peritore, Alessio Filippo
author_sort Paola, Davide Di
collection PubMed
description Imidacloprid (IMI) is part of the neonicotinoids family, insecticides widely used by humans and also found in wastewater. This class of compounds, if present in the environment, can cause toxicity to different species such as bees and gammarids, although little is known about vertebrates such as fish. In addition, several substances have been reported in the environment that can cause damage to aquatic species, such as potassium perchlorate (KClO(4)), if exposed to high concentrations or for long periods. Often, the co-presence of different contaminants can cause a synergistic action in terms of toxicity to fish. In the present study, we first analyzed different concentrations of IMI (75, 100 and 150 mg/L) and KClO(4) (1, 1.5 and 5 mM) to highlight the morphological effects at 96 hpf and, subsequently, chose two nontoxic concentrations to evaluate their co-exposure and the pathway involved in their co-toxicity. Morphological alteration, mucus production, messenger RNA (mRNA) expression related to intestinal function and oxidative stress were measured. These results suggest that co-exposure to IMI and KClO(4) could affect zebrafish embryo development by increasing gut toxicity and the alteration of antioxidative defense mechanisms.
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spelling pubmed-91452032022-05-29 Combined Effects of Potassium Perchlorate and a Neonicotinoid on Zebrafish Larvae (Danio rerio) Paola, Davide Di Capparucci, Fabiano Natale, Sabrina Crupi, Rosalia Cuzzocrea, Salvatore Spanò, Nunziacarla Gugliandolo, Enrico Peritore, Alessio Filippo Toxics Article Imidacloprid (IMI) is part of the neonicotinoids family, insecticides widely used by humans and also found in wastewater. This class of compounds, if present in the environment, can cause toxicity to different species such as bees and gammarids, although little is known about vertebrates such as fish. In addition, several substances have been reported in the environment that can cause damage to aquatic species, such as potassium perchlorate (KClO(4)), if exposed to high concentrations or for long periods. Often, the co-presence of different contaminants can cause a synergistic action in terms of toxicity to fish. In the present study, we first analyzed different concentrations of IMI (75, 100 and 150 mg/L) and KClO(4) (1, 1.5 and 5 mM) to highlight the morphological effects at 96 hpf and, subsequently, chose two nontoxic concentrations to evaluate their co-exposure and the pathway involved in their co-toxicity. Morphological alteration, mucus production, messenger RNA (mRNA) expression related to intestinal function and oxidative stress were measured. These results suggest that co-exposure to IMI and KClO(4) could affect zebrafish embryo development by increasing gut toxicity and the alteration of antioxidative defense mechanisms. MDPI 2022-04-20 /pmc/articles/PMC9145203/ /pubmed/35622618 http://dx.doi.org/10.3390/toxics10050203 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paola, Davide Di
Capparucci, Fabiano
Natale, Sabrina
Crupi, Rosalia
Cuzzocrea, Salvatore
Spanò, Nunziacarla
Gugliandolo, Enrico
Peritore, Alessio Filippo
Combined Effects of Potassium Perchlorate and a Neonicotinoid on Zebrafish Larvae (Danio rerio)
title Combined Effects of Potassium Perchlorate and a Neonicotinoid on Zebrafish Larvae (Danio rerio)
title_full Combined Effects of Potassium Perchlorate and a Neonicotinoid on Zebrafish Larvae (Danio rerio)
title_fullStr Combined Effects of Potassium Perchlorate and a Neonicotinoid on Zebrafish Larvae (Danio rerio)
title_full_unstemmed Combined Effects of Potassium Perchlorate and a Neonicotinoid on Zebrafish Larvae (Danio rerio)
title_short Combined Effects of Potassium Perchlorate and a Neonicotinoid on Zebrafish Larvae (Danio rerio)
title_sort combined effects of potassium perchlorate and a neonicotinoid on zebrafish larvae (danio rerio)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145203/
https://www.ncbi.nlm.nih.gov/pubmed/35622618
http://dx.doi.org/10.3390/toxics10050203
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