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Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust

The annual species Dasypyrum villosum possesses several potentially valuable genes for the improvement of common wheat. Previously, we identified a new stripe rust-resistant line, the Chinese Spring (CS)–D. villosum 3V#3 (3D) substitution line (named CD-3), and mapped its potential rust resistance g...

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Autores principales: Zhang, Jie, Tang, Shuyao, Lang, Tao, Wang, Ying, Long, Hai, Deng, Guangbing, Chen, Qian, Guo, Yuanlin, Xuan, Pu, Xiao, Jun, Jiang, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145344/
https://www.ncbi.nlm.nih.gov/pubmed/35631754
http://dx.doi.org/10.3390/plants11101329
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author Zhang, Jie
Tang, Shuyao
Lang, Tao
Wang, Ying
Long, Hai
Deng, Guangbing
Chen, Qian
Guo, Yuanlin
Xuan, Pu
Xiao, Jun
Jiang, Yun
author_facet Zhang, Jie
Tang, Shuyao
Lang, Tao
Wang, Ying
Long, Hai
Deng, Guangbing
Chen, Qian
Guo, Yuanlin
Xuan, Pu
Xiao, Jun
Jiang, Yun
author_sort Zhang, Jie
collection PubMed
description The annual species Dasypyrum villosum possesses several potentially valuable genes for the improvement of common wheat. Previously, we identified a new stripe rust-resistant line, the Chinese Spring (CS)–D. villosum 3V#3 (3D) substitution line (named CD-3), and mapped its potential rust resistance gene (designated as YrCD-3) on the 3V#3 chromosome originating from D. villosum. The objective of the present study was to further narrow down the YrCD-3 locus to a physical region and develop wheat-3V#3 introgression lines with strong stripe rust resistance. By treating CD-3 seeds with (60)Co γ-irradiation, two CS-3V#3 translocation lines, T3V#3S.3DL and T3DS.3V#3L (termed 22-12 and 24-20, respectively), were identified from the M(4) generation through a combination of non-denaturing fluorescence in situ hybridization (ND-FISH) and functional molecular markers. Stripe rust resistance tests showed that the line 22-12 exhibited strong stripe rust resistance similarly to CD-3, whereas 24-20 was susceptible to stripe rust similarly to CS, indicating that YrCD-3 is located on the short arm of 3V#3. The line 22-12 can potentially be used for further wheat improvement. Additionally, to trace 3V#3 in the wheat genetic background, we produced 30 3V#3-specific sequence tag (EST) markers, among which, 11 markers could identify 3V#3S. These markers could be valuable in fine-mapping YrCD-3.
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spelling pubmed-91453442022-05-29 Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust Zhang, Jie Tang, Shuyao Lang, Tao Wang, Ying Long, Hai Deng, Guangbing Chen, Qian Guo, Yuanlin Xuan, Pu Xiao, Jun Jiang, Yun Plants (Basel) Article The annual species Dasypyrum villosum possesses several potentially valuable genes for the improvement of common wheat. Previously, we identified a new stripe rust-resistant line, the Chinese Spring (CS)–D. villosum 3V#3 (3D) substitution line (named CD-3), and mapped its potential rust resistance gene (designated as YrCD-3) on the 3V#3 chromosome originating from D. villosum. The objective of the present study was to further narrow down the YrCD-3 locus to a physical region and develop wheat-3V#3 introgression lines with strong stripe rust resistance. By treating CD-3 seeds with (60)Co γ-irradiation, two CS-3V#3 translocation lines, T3V#3S.3DL and T3DS.3V#3L (termed 22-12 and 24-20, respectively), were identified from the M(4) generation through a combination of non-denaturing fluorescence in situ hybridization (ND-FISH) and functional molecular markers. Stripe rust resistance tests showed that the line 22-12 exhibited strong stripe rust resistance similarly to CD-3, whereas 24-20 was susceptible to stripe rust similarly to CS, indicating that YrCD-3 is located on the short arm of 3V#3. The line 22-12 can potentially be used for further wheat improvement. Additionally, to trace 3V#3 in the wheat genetic background, we produced 30 3V#3-specific sequence tag (EST) markers, among which, 11 markers could identify 3V#3S. These markers could be valuable in fine-mapping YrCD-3. MDPI 2022-05-18 /pmc/articles/PMC9145344/ /pubmed/35631754 http://dx.doi.org/10.3390/plants11101329 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Jie
Tang, Shuyao
Lang, Tao
Wang, Ying
Long, Hai
Deng, Guangbing
Chen, Qian
Guo, Yuanlin
Xuan, Pu
Xiao, Jun
Jiang, Yun
Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust
title Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust
title_full Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust
title_fullStr Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust
title_full_unstemmed Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust
title_short Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust
title_sort molecular cytogenetic identification of the wheat–dasypyrum villosum t3dl·3v#3s translocation line with resistance against stripe rust
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145344/
https://www.ncbi.nlm.nih.gov/pubmed/35631754
http://dx.doi.org/10.3390/plants11101329
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