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Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe
The structure and dynamics of changes in pathogen populations can be analysed by assessing the level of virulence and genetic diversity. The aim of the present study was to determine the diversity of Blumeria graminis f. sp. avenae populations. Diversity and virulence of B. graminis f. sp. avenae wa...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145444/ https://www.ncbi.nlm.nih.gov/pubmed/35631783 http://dx.doi.org/10.3390/plants11101358 |
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author | Cieplak, Magdalena Nucia, Aleksandra Ociepa, Tomasz Okoń, Sylwia |
author_facet | Cieplak, Magdalena Nucia, Aleksandra Ociepa, Tomasz Okoń, Sylwia |
author_sort | Cieplak, Magdalena |
collection | PubMed |
description | The structure and dynamics of changes in pathogen populations can be analysed by assessing the level of virulence and genetic diversity. The aim of the present study was to determine the diversity of Blumeria graminis f. sp. avenae populations. Diversity and virulence of B. graminis f. sp. avenae was assessed based on 80 single-spore isolates collected in different European countries such as Poland (40 isolates), Germany (10), Finland (10), Czech Republic (10) and Ireland (10) using ISSR (Inter-Simple Sequence Repeats) and SCoT (Start Codon Targeted) markers. This work demonstrated differences in virulence of B. graminis f. sp. avenae isolates sampled from different countries. Molecular analysis showed that both systems were useful for assessing genetic diversity, but ISSR markers were superior and generated more polymorphic products, as well as higher PIC and RP values. UPMGA and PCoA divided the isolates into groups corresponding with their geographical origin. In conclusion, the low level of genetic differentiation of the analysed isolates has suggested that the evolution of B. graminis f. sp. Avenae population is slow, and thus the evolutionary potential of the pathogen is low. This work paves the way for future studies on B. graminis f. sp. Avenae population structure and dynamics based on genetic variability. |
format | Online Article Text |
id | pubmed-9145444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91454442022-05-29 Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe Cieplak, Magdalena Nucia, Aleksandra Ociepa, Tomasz Okoń, Sylwia Plants (Basel) Article The structure and dynamics of changes in pathogen populations can be analysed by assessing the level of virulence and genetic diversity. The aim of the present study was to determine the diversity of Blumeria graminis f. sp. avenae populations. Diversity and virulence of B. graminis f. sp. avenae was assessed based on 80 single-spore isolates collected in different European countries such as Poland (40 isolates), Germany (10), Finland (10), Czech Republic (10) and Ireland (10) using ISSR (Inter-Simple Sequence Repeats) and SCoT (Start Codon Targeted) markers. This work demonstrated differences in virulence of B. graminis f. sp. avenae isolates sampled from different countries. Molecular analysis showed that both systems were useful for assessing genetic diversity, but ISSR markers were superior and generated more polymorphic products, as well as higher PIC and RP values. UPMGA and PCoA divided the isolates into groups corresponding with their geographical origin. In conclusion, the low level of genetic differentiation of the analysed isolates has suggested that the evolution of B. graminis f. sp. Avenae population is slow, and thus the evolutionary potential of the pathogen is low. This work paves the way for future studies on B. graminis f. sp. Avenae population structure and dynamics based on genetic variability. MDPI 2022-05-20 /pmc/articles/PMC9145444/ /pubmed/35631783 http://dx.doi.org/10.3390/plants11101358 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cieplak, Magdalena Nucia, Aleksandra Ociepa, Tomasz Okoń, Sylwia Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe |
title | Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe |
title_full | Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe |
title_fullStr | Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe |
title_full_unstemmed | Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe |
title_short | Virulence Structure and Genetic Diversity of Blumeria graminis f. sp. avenae from Different Regions of Europe |
title_sort | virulence structure and genetic diversity of blumeria graminis f. sp. avenae from different regions of europe |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145444/ https://www.ncbi.nlm.nih.gov/pubmed/35631783 http://dx.doi.org/10.3390/plants11101358 |
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