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Carry-Over of Zearalenone and Its Metabolites to Intestinal Tissues and the Expression of CYP1A1 and GSTπ1 in the Colon of Gilts before Puberty

The objective of this study was to evaluate whether low doses of zearalenone (ZEN) affect the carry-over of ZEN and its metabolites to intestinal tissues and the expression of CYP1A1 and GSTπ1 in the large intestine. Prepubertal gilts (with a BW of up to 14.5 kg) were exposed in group ZEN to daily Z...

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Autores principales: Mróz, Magdalena, Gajęcka, Magdalena, Brzuzan, Paweł, Lisieska-Żołnierczyk, Sylwia, Leski, Dawid, Zielonka, Łukasz, Gajęcki, Maciej T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145504/
https://www.ncbi.nlm.nih.gov/pubmed/35622600
http://dx.doi.org/10.3390/toxins14050354
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author Mróz, Magdalena
Gajęcka, Magdalena
Brzuzan, Paweł
Lisieska-Żołnierczyk, Sylwia
Leski, Dawid
Zielonka, Łukasz
Gajęcki, Maciej T.
author_facet Mróz, Magdalena
Gajęcka, Magdalena
Brzuzan, Paweł
Lisieska-Żołnierczyk, Sylwia
Leski, Dawid
Zielonka, Łukasz
Gajęcki, Maciej T.
author_sort Mróz, Magdalena
collection PubMed
description The objective of this study was to evaluate whether low doses of zearalenone (ZEN) affect the carry-over of ZEN and its metabolites to intestinal tissues and the expression of CYP1A1 and GSTπ1 in the large intestine. Prepubertal gilts (with a BW of up to 14.5 kg) were exposed in group ZEN to daily ZEN5 doses of 5 μg/kg BW (n = 15); in group ZEN10, 10 μg/kg BW (n = 15); in group ZEN15, 15 μg/kg BW (n = 15); or were administered a placebo (group C, n = 15) throughout the experiment. After euthanasia, tissues were sampled on exposure days 7, 21, and 42 (D1, D2, and D3, respectively). The results confirmed that the administered ZEN doses (LOAEL, NOAEL, and MABEL) were appropriate to reliably assess the carry-over of ZEN. Based on the observations made during 42 days of exposure to pure ZEN, it can be hypothesized that all mycotoxins (ZEN, α-zearalenol, and β-zearalenol) contribute to a balance between intestinal cells and the expression of selected genes encoding enzymes that participate in biotransformation processes in the large intestine; modulate feminization processes in prepubertal gilts; and elicit flexible, adaptive responses of the macroorganism to mycotoxin exposure at the analyzed doses.
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spelling pubmed-91455042022-05-29 Carry-Over of Zearalenone and Its Metabolites to Intestinal Tissues and the Expression of CYP1A1 and GSTπ1 in the Colon of Gilts before Puberty Mróz, Magdalena Gajęcka, Magdalena Brzuzan, Paweł Lisieska-Żołnierczyk, Sylwia Leski, Dawid Zielonka, Łukasz Gajęcki, Maciej T. Toxins (Basel) Article The objective of this study was to evaluate whether low doses of zearalenone (ZEN) affect the carry-over of ZEN and its metabolites to intestinal tissues and the expression of CYP1A1 and GSTπ1 in the large intestine. Prepubertal gilts (with a BW of up to 14.5 kg) were exposed in group ZEN to daily ZEN5 doses of 5 μg/kg BW (n = 15); in group ZEN10, 10 μg/kg BW (n = 15); in group ZEN15, 15 μg/kg BW (n = 15); or were administered a placebo (group C, n = 15) throughout the experiment. After euthanasia, tissues were sampled on exposure days 7, 21, and 42 (D1, D2, and D3, respectively). The results confirmed that the administered ZEN doses (LOAEL, NOAEL, and MABEL) were appropriate to reliably assess the carry-over of ZEN. Based on the observations made during 42 days of exposure to pure ZEN, it can be hypothesized that all mycotoxins (ZEN, α-zearalenol, and β-zearalenol) contribute to a balance between intestinal cells and the expression of selected genes encoding enzymes that participate in biotransformation processes in the large intestine; modulate feminization processes in prepubertal gilts; and elicit flexible, adaptive responses of the macroorganism to mycotoxin exposure at the analyzed doses. MDPI 2022-05-18 /pmc/articles/PMC9145504/ /pubmed/35622600 http://dx.doi.org/10.3390/toxins14050354 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mróz, Magdalena
Gajęcka, Magdalena
Brzuzan, Paweł
Lisieska-Żołnierczyk, Sylwia
Leski, Dawid
Zielonka, Łukasz
Gajęcki, Maciej T.
Carry-Over of Zearalenone and Its Metabolites to Intestinal Tissues and the Expression of CYP1A1 and GSTπ1 in the Colon of Gilts before Puberty
title Carry-Over of Zearalenone and Its Metabolites to Intestinal Tissues and the Expression of CYP1A1 and GSTπ1 in the Colon of Gilts before Puberty
title_full Carry-Over of Zearalenone and Its Metabolites to Intestinal Tissues and the Expression of CYP1A1 and GSTπ1 in the Colon of Gilts before Puberty
title_fullStr Carry-Over of Zearalenone and Its Metabolites to Intestinal Tissues and the Expression of CYP1A1 and GSTπ1 in the Colon of Gilts before Puberty
title_full_unstemmed Carry-Over of Zearalenone and Its Metabolites to Intestinal Tissues and the Expression of CYP1A1 and GSTπ1 in the Colon of Gilts before Puberty
title_short Carry-Over of Zearalenone and Its Metabolites to Intestinal Tissues and the Expression of CYP1A1 and GSTπ1 in the Colon of Gilts before Puberty
title_sort carry-over of zearalenone and its metabolites to intestinal tissues and the expression of cyp1a1 and gstπ1 in the colon of gilts before puberty
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145504/
https://www.ncbi.nlm.nih.gov/pubmed/35622600
http://dx.doi.org/10.3390/toxins14050354
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