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Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas
The poly(A) tail at the 3′ end of mRNAs determines their stability, translational efficiency, and fate. The shortening of the poly(A) tail, and its efficient removal, triggers the degradation of mRNAs, thus, regulating gene expression. The process is catalyzed by a family of enzymes, known as deaden...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145511/ https://www.ncbi.nlm.nih.gov/pubmed/35630580 http://dx.doi.org/10.3390/molecules27103102 |
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author | Kyritsis, Athanasios Papanastasi, Eirini Kokkori, Ioanna Maragozidis, Panagiotis Chatzileontiadou, Demetra S. M. Pallaki, Paschalina Labrou, Maria Zarogiannis, Sotirios G. Chrousos, George P. Vlachakis, Dimitrios Gourgoulianis, Konstantinos I. Balatsos, Nikolaos A. A. |
author_facet | Kyritsis, Athanasios Papanastasi, Eirini Kokkori, Ioanna Maragozidis, Panagiotis Chatzileontiadou, Demetra S. M. Pallaki, Paschalina Labrou, Maria Zarogiannis, Sotirios G. Chrousos, George P. Vlachakis, Dimitrios Gourgoulianis, Konstantinos I. Balatsos, Nikolaos A. A. |
author_sort | Kyritsis, Athanasios |
collection | PubMed |
description | The poly(A) tail at the 3′ end of mRNAs determines their stability, translational efficiency, and fate. The shortening of the poly(A) tail, and its efficient removal, triggers the degradation of mRNAs, thus, regulating gene expression. The process is catalyzed by a family of enzymes, known as deadenylases. As the dysregulation of gene expression is a hallmark of cancer, understanding the role of deadenylases has gained additional interest. Herein, the genetic association network shows that CNOT6 and CNOT7 are the most prevalent and most interconnected nodes in the equilibrated diagram. Subsequent silencing and transcriptomic analysis identifies transcripts possibly regulated by specific deadenylases. Furthermore, several gene ontologies are enriched by common deregulated genes. Given the potential concerted action and overlapping functions of deadenylases, we examined the effect of silencing a deadenylase on the remaining ones. Our results suggest that specific deadenylases target unique subsets of mRNAs, whilst at the same time, multiple deadenylases may affect the same mRNAs with overlapping functions. |
format | Online Article Text |
id | pubmed-9145511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91455112022-05-29 Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas Kyritsis, Athanasios Papanastasi, Eirini Kokkori, Ioanna Maragozidis, Panagiotis Chatzileontiadou, Demetra S. M. Pallaki, Paschalina Labrou, Maria Zarogiannis, Sotirios G. Chrousos, George P. Vlachakis, Dimitrios Gourgoulianis, Konstantinos I. Balatsos, Nikolaos A. A. Molecules Article The poly(A) tail at the 3′ end of mRNAs determines their stability, translational efficiency, and fate. The shortening of the poly(A) tail, and its efficient removal, triggers the degradation of mRNAs, thus, regulating gene expression. The process is catalyzed by a family of enzymes, known as deadenylases. As the dysregulation of gene expression is a hallmark of cancer, understanding the role of deadenylases has gained additional interest. Herein, the genetic association network shows that CNOT6 and CNOT7 are the most prevalent and most interconnected nodes in the equilibrated diagram. Subsequent silencing and transcriptomic analysis identifies transcripts possibly regulated by specific deadenylases. Furthermore, several gene ontologies are enriched by common deregulated genes. Given the potential concerted action and overlapping functions of deadenylases, we examined the effect of silencing a deadenylase on the remaining ones. Our results suggest that specific deadenylases target unique subsets of mRNAs, whilst at the same time, multiple deadenylases may affect the same mRNAs with overlapping functions. MDPI 2022-05-12 /pmc/articles/PMC9145511/ /pubmed/35630580 http://dx.doi.org/10.3390/molecules27103102 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kyritsis, Athanasios Papanastasi, Eirini Kokkori, Ioanna Maragozidis, Panagiotis Chatzileontiadou, Demetra S. M. Pallaki, Paschalina Labrou, Maria Zarogiannis, Sotirios G. Chrousos, George P. Vlachakis, Dimitrios Gourgoulianis, Konstantinos I. Balatsos, Nikolaos A. A. Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas |
title | Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas |
title_full | Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas |
title_fullStr | Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas |
title_full_unstemmed | Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas |
title_short | Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas |
title_sort | integrated deadenylase genetic association network and transcriptome analysis in thoracic carcinomas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145511/ https://www.ncbi.nlm.nih.gov/pubmed/35630580 http://dx.doi.org/10.3390/molecules27103102 |
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