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NK Cells Acquire CCR5 and CXCR4 by Trogocytosis in People Living with HIV-1

NK cells play a major role in the antiviral immune response, including against HIV-1. HIV-1 patients have impaired NK cell activity with a decrease in CD56(dim) NK cells and an increase in the CD56(−)CD16(+) subset, and recently it has been proposed that a population of CD56(+)NKG2C(+)KIR(+)CD57(+)...

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Autores principales: Vo, Dang-Nghiem, Leventoux, Nicolas, Campos-Mora, Mauricio, Gimenez, Sandrine, Corbeau, Pierre, Villalba, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145773/
https://www.ncbi.nlm.nih.gov/pubmed/35632444
http://dx.doi.org/10.3390/vaccines10050688
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author Vo, Dang-Nghiem
Leventoux, Nicolas
Campos-Mora, Mauricio
Gimenez, Sandrine
Corbeau, Pierre
Villalba, Martin
author_facet Vo, Dang-Nghiem
Leventoux, Nicolas
Campos-Mora, Mauricio
Gimenez, Sandrine
Corbeau, Pierre
Villalba, Martin
author_sort Vo, Dang-Nghiem
collection PubMed
description NK cells play a major role in the antiviral immune response, including against HIV-1. HIV-1 patients have impaired NK cell activity with a decrease in CD56(dim) NK cells and an increase in the CD56(−)CD16(+) subset, and recently it has been proposed that a population of CD56(+)NKG2C(+)KIR(+)CD57(+) cells represents antiviral memory NK cells. Antiretroviral therapy (ART) partly restores the functional activity of this lymphocyte lineage. NK cells when interacting with their targets can gain antigens from them by the process of trogocytosis. Here we show that NK cells can obtain CCR5 and CXCR4, but barely CD4, from T cell lines by trogocytosis in vitro. By UMAP (Uniform Manifold Approximation and Projection), we show that aviremic HIV-1 patients have unique NK cell clusters that include cells expressing CCR5, NKG2C and KIRs, but lack CD57 expression. Viremic patients have a larger proportion of CXCR4(+) and CCR5(+) NK cells than healthy donors (HD) and this is largely increased in CD107(+) cells, suggesting a link between degranulation and trogocytosis. In agreement, UMAP identified a specific NK cell cluster in viremic HIV-1 patients, which contains most of the CD107a(+), CCR5(+) and CXCR4(+) cells. However, this cluster lacks NKG2C expression. Therefore, NK cells can gain CCR5 and CXCR4 by trogocytosis, which depends on degranulation.
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spelling pubmed-91457732022-05-29 NK Cells Acquire CCR5 and CXCR4 by Trogocytosis in People Living with HIV-1 Vo, Dang-Nghiem Leventoux, Nicolas Campos-Mora, Mauricio Gimenez, Sandrine Corbeau, Pierre Villalba, Martin Vaccines (Basel) Article NK cells play a major role in the antiviral immune response, including against HIV-1. HIV-1 patients have impaired NK cell activity with a decrease in CD56(dim) NK cells and an increase in the CD56(−)CD16(+) subset, and recently it has been proposed that a population of CD56(+)NKG2C(+)KIR(+)CD57(+) cells represents antiviral memory NK cells. Antiretroviral therapy (ART) partly restores the functional activity of this lymphocyte lineage. NK cells when interacting with their targets can gain antigens from them by the process of trogocytosis. Here we show that NK cells can obtain CCR5 and CXCR4, but barely CD4, from T cell lines by trogocytosis in vitro. By UMAP (Uniform Manifold Approximation and Projection), we show that aviremic HIV-1 patients have unique NK cell clusters that include cells expressing CCR5, NKG2C and KIRs, but lack CD57 expression. Viremic patients have a larger proportion of CXCR4(+) and CCR5(+) NK cells than healthy donors (HD) and this is largely increased in CD107(+) cells, suggesting a link between degranulation and trogocytosis. In agreement, UMAP identified a specific NK cell cluster in viremic HIV-1 patients, which contains most of the CD107a(+), CCR5(+) and CXCR4(+) cells. However, this cluster lacks NKG2C expression. Therefore, NK cells can gain CCR5 and CXCR4 by trogocytosis, which depends on degranulation. MDPI 2022-04-28 /pmc/articles/PMC9145773/ /pubmed/35632444 http://dx.doi.org/10.3390/vaccines10050688 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vo, Dang-Nghiem
Leventoux, Nicolas
Campos-Mora, Mauricio
Gimenez, Sandrine
Corbeau, Pierre
Villalba, Martin
NK Cells Acquire CCR5 and CXCR4 by Trogocytosis in People Living with HIV-1
title NK Cells Acquire CCR5 and CXCR4 by Trogocytosis in People Living with HIV-1
title_full NK Cells Acquire CCR5 and CXCR4 by Trogocytosis in People Living with HIV-1
title_fullStr NK Cells Acquire CCR5 and CXCR4 by Trogocytosis in People Living with HIV-1
title_full_unstemmed NK Cells Acquire CCR5 and CXCR4 by Trogocytosis in People Living with HIV-1
title_short NK Cells Acquire CCR5 and CXCR4 by Trogocytosis in People Living with HIV-1
title_sort nk cells acquire ccr5 and cxcr4 by trogocytosis in people living with hiv-1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145773/
https://www.ncbi.nlm.nih.gov/pubmed/35632444
http://dx.doi.org/10.3390/vaccines10050688
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