Cargando…

The Highly Leukotoxic JP2 Genotype of Aggregatibacter actinomycetemcomitans Is Present in the Population of the West African Island, Sal in Cape Verde: A Pilot Study

Aggregatibacter actinomycetemcomitans is strongly associated with severe periodontitis, possibly due to its production of a potent leukotoxin. A genetic variant, the JP2 genotype, was found to produce more leukotoxin than the wild type because of a mutation in the leukotoxin gene, and this genotype...

Descripción completa

Detalles Bibliográficos
Autores principales: De Soet, Johannes J., Claesson, Rolf, Haubek, Dorte, Johansson, Anders, Buijs, Mark J., Volgenant, Catherine M. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145930/
https://www.ncbi.nlm.nih.gov/pubmed/35631098
http://dx.doi.org/10.3390/pathogens11050577
_version_ 1784716435990249472
author De Soet, Johannes J.
Claesson, Rolf
Haubek, Dorte
Johansson, Anders
Buijs, Mark J.
Volgenant, Catherine M. C.
author_facet De Soet, Johannes J.
Claesson, Rolf
Haubek, Dorte
Johansson, Anders
Buijs, Mark J.
Volgenant, Catherine M. C.
author_sort De Soet, Johannes J.
collection PubMed
description Aggregatibacter actinomycetemcomitans is strongly associated with severe periodontitis, possibly due to its production of a potent leukotoxin. A genetic variant, the JP2 genotype, was found to produce more leukotoxin than the wild type because of a mutation in the leukotoxin gene, and this genotype is frequently found in African populations. The aim of this study was to investigate whether this JP2 genotype can be found in a randomly selected group of inhabitants of Sal, Cape Verde. Twenty-nine adults between 20 and 59 years of age (58.6% female) participated, and information on their oral health and living standards was collected. An oral examination was performed for each participant, including DMF-T and CPI scores. Plaque and saliva samples were collected and transported to Europe, where DNA was isolated, and the concentration of A. actinomycetemcomitans and its JP2 genotype was determined using dedicated PCR analyses. All 29 plaque and 31% of the saliva samples harboured A. actinomycetemcomitans, and two participants were positive for the JP2 genotype. The presence of this JP2 genotype was not associated with either CPI or DMF-T. This pilot study is the first to describe the presence of the A. actinomycetemcomitans JP2 genotype in a Cape Verdean population living in the Cape Verde Islands, and the findings warrant further research.
format Online
Article
Text
id pubmed-9145930
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91459302022-05-29 The Highly Leukotoxic JP2 Genotype of Aggregatibacter actinomycetemcomitans Is Present in the Population of the West African Island, Sal in Cape Verde: A Pilot Study De Soet, Johannes J. Claesson, Rolf Haubek, Dorte Johansson, Anders Buijs, Mark J. Volgenant, Catherine M. C. Pathogens Brief Report Aggregatibacter actinomycetemcomitans is strongly associated with severe periodontitis, possibly due to its production of a potent leukotoxin. A genetic variant, the JP2 genotype, was found to produce more leukotoxin than the wild type because of a mutation in the leukotoxin gene, and this genotype is frequently found in African populations. The aim of this study was to investigate whether this JP2 genotype can be found in a randomly selected group of inhabitants of Sal, Cape Verde. Twenty-nine adults between 20 and 59 years of age (58.6% female) participated, and information on their oral health and living standards was collected. An oral examination was performed for each participant, including DMF-T and CPI scores. Plaque and saliva samples were collected and transported to Europe, where DNA was isolated, and the concentration of A. actinomycetemcomitans and its JP2 genotype was determined using dedicated PCR analyses. All 29 plaque and 31% of the saliva samples harboured A. actinomycetemcomitans, and two participants were positive for the JP2 genotype. The presence of this JP2 genotype was not associated with either CPI or DMF-T. This pilot study is the first to describe the presence of the A. actinomycetemcomitans JP2 genotype in a Cape Verdean population living in the Cape Verde Islands, and the findings warrant further research. MDPI 2022-05-13 /pmc/articles/PMC9145930/ /pubmed/35631098 http://dx.doi.org/10.3390/pathogens11050577 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
De Soet, Johannes J.
Claesson, Rolf
Haubek, Dorte
Johansson, Anders
Buijs, Mark J.
Volgenant, Catherine M. C.
The Highly Leukotoxic JP2 Genotype of Aggregatibacter actinomycetemcomitans Is Present in the Population of the West African Island, Sal in Cape Verde: A Pilot Study
title The Highly Leukotoxic JP2 Genotype of Aggregatibacter actinomycetemcomitans Is Present in the Population of the West African Island, Sal in Cape Verde: A Pilot Study
title_full The Highly Leukotoxic JP2 Genotype of Aggregatibacter actinomycetemcomitans Is Present in the Population of the West African Island, Sal in Cape Verde: A Pilot Study
title_fullStr The Highly Leukotoxic JP2 Genotype of Aggregatibacter actinomycetemcomitans Is Present in the Population of the West African Island, Sal in Cape Verde: A Pilot Study
title_full_unstemmed The Highly Leukotoxic JP2 Genotype of Aggregatibacter actinomycetemcomitans Is Present in the Population of the West African Island, Sal in Cape Verde: A Pilot Study
title_short The Highly Leukotoxic JP2 Genotype of Aggregatibacter actinomycetemcomitans Is Present in the Population of the West African Island, Sal in Cape Verde: A Pilot Study
title_sort highly leukotoxic jp2 genotype of aggregatibacter actinomycetemcomitans is present in the population of the west african island, sal in cape verde: a pilot study
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145930/
https://www.ncbi.nlm.nih.gov/pubmed/35631098
http://dx.doi.org/10.3390/pathogens11050577
work_keys_str_mv AT desoetjohannesj thehighlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT claessonrolf thehighlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT haubekdorte thehighlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT johanssonanders thehighlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT buijsmarkj thehighlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT volgenantcatherinemc thehighlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT desoetjohannesj highlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT claessonrolf highlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT haubekdorte highlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT johanssonanders highlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT buijsmarkj highlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy
AT volgenantcatherinemc highlyleukotoxicjp2genotypeofaggregatibacteractinomycetemcomitansispresentinthepopulationofthewestafricanislandsalincapeverdeapilotstudy