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B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection
A new antibody diagnostic assay with more rapid and robust properties is demanded to quantitatively evaluate anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunity in a large population. Here, we developed a nanometer-scale fluorescent biosensor system consisting of CdSe-ZnS quan...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145955/ https://www.ncbi.nlm.nih.gov/pubmed/35632772 http://dx.doi.org/10.3390/v14051031 |
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author | Zheng, Yucheng Song, Kun Cai, Kun Liu, Linlin Tang, Dixiao Long, Wenbo Zhai, Bohui Chen, Jianjun Tao, Yanbing Zhao, Yunong Liang, Simeng Huang, Qing Liu, Qianyun Zhang, Qi Chen, Yu Liu, Yingle Li, Huayao Wang, Ping Lan, Ke Liu, Huan Xu, Ke |
author_facet | Zheng, Yucheng Song, Kun Cai, Kun Liu, Linlin Tang, Dixiao Long, Wenbo Zhai, Bohui Chen, Jianjun Tao, Yanbing Zhao, Yunong Liang, Simeng Huang, Qing Liu, Qianyun Zhang, Qi Chen, Yu Liu, Yingle Li, Huayao Wang, Ping Lan, Ke Liu, Huan Xu, Ke |
author_sort | Zheng, Yucheng |
collection | PubMed |
description | A new antibody diagnostic assay with more rapid and robust properties is demanded to quantitatively evaluate anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunity in a large population. Here, we developed a nanometer-scale fluorescent biosensor system consisting of CdSe-ZnS quantum dots (QDs) coupled with the highly sensitive B-cell epitopes of SARS-CoV-2 that could remarkably identify the corresponding antibody with a detection limit of 100 pM. Intriguingly, we found that fluorescence quenching of QDs was stimulated more obviously when coupled with peptides than the corresponding proteins, indicating that the energy transfer between QDs and peptides was more effective. Compared to the traditional enzyme-linked immunosorbent assay (ELISA), the B-cell-epitope-based QD-biosensor could robustly distinguish coronavirus disease 2019 (COVID-19) antibody-positive patients from uninfected individuals with a higher sensitivity (92.3–98.1% positive rates by QD-biosensor vs. 78.3–83.1% positive rates by ELISAs in 207 COVID-19 patients’ sera) in a more rapid (5 min) and labor-saving manner. Taken together, the ‘QD-peptides’ biosensor provided a novel real-time, quantitative, and high-throughput method for clinical diagnosis and home-use tests. |
format | Online Article Text |
id | pubmed-9145955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91459552022-05-29 B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection Zheng, Yucheng Song, Kun Cai, Kun Liu, Linlin Tang, Dixiao Long, Wenbo Zhai, Bohui Chen, Jianjun Tao, Yanbing Zhao, Yunong Liang, Simeng Huang, Qing Liu, Qianyun Zhang, Qi Chen, Yu Liu, Yingle Li, Huayao Wang, Ping Lan, Ke Liu, Huan Xu, Ke Viruses Article A new antibody diagnostic assay with more rapid and robust properties is demanded to quantitatively evaluate anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunity in a large population. Here, we developed a nanometer-scale fluorescent biosensor system consisting of CdSe-ZnS quantum dots (QDs) coupled with the highly sensitive B-cell epitopes of SARS-CoV-2 that could remarkably identify the corresponding antibody with a detection limit of 100 pM. Intriguingly, we found that fluorescence quenching of QDs was stimulated more obviously when coupled with peptides than the corresponding proteins, indicating that the energy transfer between QDs and peptides was more effective. Compared to the traditional enzyme-linked immunosorbent assay (ELISA), the B-cell-epitope-based QD-biosensor could robustly distinguish coronavirus disease 2019 (COVID-19) antibody-positive patients from uninfected individuals with a higher sensitivity (92.3–98.1% positive rates by QD-biosensor vs. 78.3–83.1% positive rates by ELISAs in 207 COVID-19 patients’ sera) in a more rapid (5 min) and labor-saving manner. Taken together, the ‘QD-peptides’ biosensor provided a novel real-time, quantitative, and high-throughput method for clinical diagnosis and home-use tests. MDPI 2022-05-12 /pmc/articles/PMC9145955/ /pubmed/35632772 http://dx.doi.org/10.3390/v14051031 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zheng, Yucheng Song, Kun Cai, Kun Liu, Linlin Tang, Dixiao Long, Wenbo Zhai, Bohui Chen, Jianjun Tao, Yanbing Zhao, Yunong Liang, Simeng Huang, Qing Liu, Qianyun Zhang, Qi Chen, Yu Liu, Yingle Li, Huayao Wang, Ping Lan, Ke Liu, Huan Xu, Ke B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection |
title | B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection |
title_full | B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection |
title_fullStr | B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection |
title_full_unstemmed | B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection |
title_short | B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection |
title_sort | b-cell-epitope-based fluorescent quantum dot biosensors for sars-cov-2 enable highly sensitive covid-19 antibody detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145955/ https://www.ncbi.nlm.nih.gov/pubmed/35632772 http://dx.doi.org/10.3390/v14051031 |
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