Cargando…

Genomic Analysis of Endophytic Bacillus-Related Strains Isolated from the Medicinal Plant Origanum vulgare L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds

Multidrug-resistant pathogens represent a serious threat to human health. The inefficacy of traditional antibiotic drugs could be surmounted through the exploitation of natural bioactive compounds of which medicinal plants are a great reservoir. The finding that bacteria living inside plant tissues,...

Descripción completa

Detalles Bibliográficos
Autores principales: Semenzato, Giulia, Alonso-Vásquez, Tania, Del Duca, Sara, Vassallo, Alberto, Riccardi, Christopher, Zaccaroni, Marco, Mucci, Nadia, Padula, Anna, Emiliani, Giovanni, Palumbo Piccionello, Antonio, Puglia, Anna Maria, Fani, Renato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145963/
https://www.ncbi.nlm.nih.gov/pubmed/35630363
http://dx.doi.org/10.3390/microorganisms10050919
_version_ 1784716443983544320
author Semenzato, Giulia
Alonso-Vásquez, Tania
Del Duca, Sara
Vassallo, Alberto
Riccardi, Christopher
Zaccaroni, Marco
Mucci, Nadia
Padula, Anna
Emiliani, Giovanni
Palumbo Piccionello, Antonio
Puglia, Anna Maria
Fani, Renato
author_facet Semenzato, Giulia
Alonso-Vásquez, Tania
Del Duca, Sara
Vassallo, Alberto
Riccardi, Christopher
Zaccaroni, Marco
Mucci, Nadia
Padula, Anna
Emiliani, Giovanni
Palumbo Piccionello, Antonio
Puglia, Anna Maria
Fani, Renato
author_sort Semenzato, Giulia
collection PubMed
description Multidrug-resistant pathogens represent a serious threat to human health. The inefficacy of traditional antibiotic drugs could be surmounted through the exploitation of natural bioactive compounds of which medicinal plants are a great reservoir. The finding that bacteria living inside plant tissues, (i.e., the endophytic bacterial microbiome) can influence the synthesis of the aforementioned compounds leads to the necessity of unraveling the mechanisms involved in the determination of this symbiotic relationship. Here, we report the genome sequence of four endophytic bacterial strains isolated from the medicinal plant Origanum vulgare L. and able to antagonize the growth of opportunistic pathogens of cystic fibrosis patients. The in silico analysis revealed the presence of gene clusters involved in the production of antimicrobial compounds, such as paeninodin, paenilarvins, polymyxin, and paenicidin A. Endophytes’ adaptation to the plant microenvironment was evaluated through the analysis of the presence of antibiotic resistance genes in the four genomes. The diesel fuel degrading potential was also tested. Strains grew in minimum media supplemented with diesel fuel, but no n-alkanes degradation genes were found in their genomes, suggesting that diesel fuel degradation might occur through other steps involving enzymes catalyzing the oxidation of aromatic compounds.
format Online
Article
Text
id pubmed-9145963
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91459632022-05-29 Genomic Analysis of Endophytic Bacillus-Related Strains Isolated from the Medicinal Plant Origanum vulgare L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds Semenzato, Giulia Alonso-Vásquez, Tania Del Duca, Sara Vassallo, Alberto Riccardi, Christopher Zaccaroni, Marco Mucci, Nadia Padula, Anna Emiliani, Giovanni Palumbo Piccionello, Antonio Puglia, Anna Maria Fani, Renato Microorganisms Article Multidrug-resistant pathogens represent a serious threat to human health. The inefficacy of traditional antibiotic drugs could be surmounted through the exploitation of natural bioactive compounds of which medicinal plants are a great reservoir. The finding that bacteria living inside plant tissues, (i.e., the endophytic bacterial microbiome) can influence the synthesis of the aforementioned compounds leads to the necessity of unraveling the mechanisms involved in the determination of this symbiotic relationship. Here, we report the genome sequence of four endophytic bacterial strains isolated from the medicinal plant Origanum vulgare L. and able to antagonize the growth of opportunistic pathogens of cystic fibrosis patients. The in silico analysis revealed the presence of gene clusters involved in the production of antimicrobial compounds, such as paeninodin, paenilarvins, polymyxin, and paenicidin A. Endophytes’ adaptation to the plant microenvironment was evaluated through the analysis of the presence of antibiotic resistance genes in the four genomes. The diesel fuel degrading potential was also tested. Strains grew in minimum media supplemented with diesel fuel, but no n-alkanes degradation genes were found in their genomes, suggesting that diesel fuel degradation might occur through other steps involving enzymes catalyzing the oxidation of aromatic compounds. MDPI 2022-04-27 /pmc/articles/PMC9145963/ /pubmed/35630363 http://dx.doi.org/10.3390/microorganisms10050919 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Semenzato, Giulia
Alonso-Vásquez, Tania
Del Duca, Sara
Vassallo, Alberto
Riccardi, Christopher
Zaccaroni, Marco
Mucci, Nadia
Padula, Anna
Emiliani, Giovanni
Palumbo Piccionello, Antonio
Puglia, Anna Maria
Fani, Renato
Genomic Analysis of Endophytic Bacillus-Related Strains Isolated from the Medicinal Plant Origanum vulgare L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds
title Genomic Analysis of Endophytic Bacillus-Related Strains Isolated from the Medicinal Plant Origanum vulgare L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds
title_full Genomic Analysis of Endophytic Bacillus-Related Strains Isolated from the Medicinal Plant Origanum vulgare L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds
title_fullStr Genomic Analysis of Endophytic Bacillus-Related Strains Isolated from the Medicinal Plant Origanum vulgare L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds
title_full_unstemmed Genomic Analysis of Endophytic Bacillus-Related Strains Isolated from the Medicinal Plant Origanum vulgare L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds
title_short Genomic Analysis of Endophytic Bacillus-Related Strains Isolated from the Medicinal Plant Origanum vulgare L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds
title_sort genomic analysis of endophytic bacillus-related strains isolated from the medicinal plant origanum vulgare l. revealed the presence of metabolic pathways involved in the biosynthesis of bioactive compounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145963/
https://www.ncbi.nlm.nih.gov/pubmed/35630363
http://dx.doi.org/10.3390/microorganisms10050919
work_keys_str_mv AT semenzatogiulia genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT alonsovasqueztania genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT delducasara genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT vassalloalberto genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT riccardichristopher genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT zaccaronimarco genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT muccinadia genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT padulaanna genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT emilianigiovanni genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT palumbopiccionelloantonio genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT pugliaannamaria genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds
AT fanirenato genomicanalysisofendophyticbacillusrelatedstrainsisolatedfromthemedicinalplantoriganumvulgarelrevealedthepresenceofmetabolicpathwaysinvolvedinthebiosynthesisofbioactivecompounds