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Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds

The naturally occurring dipeptide carnosine (β-alanyl-L-histidine) specifically attenuates tumor growth. Here, we ask whether other small imidazole-containing compounds also affect the viability of tumor cells without affecting non-malignant cells and whether the formation of histamine is involved....

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Autores principales: Seidel, Elisabeth Christiane, Birkemeyer, Claudia, Baran-Schmidt, Rainer, Meixensberger, Jürgen, Oppermann, Henry, Gaunitz, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146156/
https://www.ncbi.nlm.nih.gov/pubmed/35628643
http://dx.doi.org/10.3390/ijms23105834
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author Seidel, Elisabeth Christiane
Birkemeyer, Claudia
Baran-Schmidt, Rainer
Meixensberger, Jürgen
Oppermann, Henry
Gaunitz, Frank
author_facet Seidel, Elisabeth Christiane
Birkemeyer, Claudia
Baran-Schmidt, Rainer
Meixensberger, Jürgen
Oppermann, Henry
Gaunitz, Frank
author_sort Seidel, Elisabeth Christiane
collection PubMed
description The naturally occurring dipeptide carnosine (β-alanyl-L-histidine) specifically attenuates tumor growth. Here, we ask whether other small imidazole-containing compounds also affect the viability of tumor cells without affecting non-malignant cells and whether the formation of histamine is involved. Patient-derived fibroblasts and glioblastoma cells were treated with carnosine, L-alanyl-L-histidine (LA-LH), β-alanyl-L-alanine, L-histidine, histamine, imidazole, β-alanine, and L-alanine. Cell viability was assessed by cell-based assays and microscopy. The intracellular release of L-histidine and formation of histamine was investigated by high-performance liquid chromatography coupled to mass spectrometry. Carnosine and LA-LH inhibited tumor cell growth with minor effects on fibroblasts, and L-histidine, histamine, and imidazole affected viability in both cell types. Compounds without the imidazole moiety did not diminish viability. In the presence of LA-LH but not in the presence of carnosine, a significant rise in intracellular amounts of histidine was detected in all cells. The formation of histamine was not detectable in the presence of carnosine, LA-LH, or histidine. In conclusion, the imidazole moiety of carnosine contributes to its anti-neoplastic effect, which is also seen in the presence of histidine and LA-LH. Despite the fact that histamine has a strong effect on cell viability, the formation of histamine is not responsible for the effects on the cell viability of carnosine, LA-LH, and histidine.
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spelling pubmed-91461562022-05-29 Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds Seidel, Elisabeth Christiane Birkemeyer, Claudia Baran-Schmidt, Rainer Meixensberger, Jürgen Oppermann, Henry Gaunitz, Frank Int J Mol Sci Article The naturally occurring dipeptide carnosine (β-alanyl-L-histidine) specifically attenuates tumor growth. Here, we ask whether other small imidazole-containing compounds also affect the viability of tumor cells without affecting non-malignant cells and whether the formation of histamine is involved. Patient-derived fibroblasts and glioblastoma cells were treated with carnosine, L-alanyl-L-histidine (LA-LH), β-alanyl-L-alanine, L-histidine, histamine, imidazole, β-alanine, and L-alanine. Cell viability was assessed by cell-based assays and microscopy. The intracellular release of L-histidine and formation of histamine was investigated by high-performance liquid chromatography coupled to mass spectrometry. Carnosine and LA-LH inhibited tumor cell growth with minor effects on fibroblasts, and L-histidine, histamine, and imidazole affected viability in both cell types. Compounds without the imidazole moiety did not diminish viability. In the presence of LA-LH but not in the presence of carnosine, a significant rise in intracellular amounts of histidine was detected in all cells. The formation of histamine was not detectable in the presence of carnosine, LA-LH, or histidine. In conclusion, the imidazole moiety of carnosine contributes to its anti-neoplastic effect, which is also seen in the presence of histidine and LA-LH. Despite the fact that histamine has a strong effect on cell viability, the formation of histamine is not responsible for the effects on the cell viability of carnosine, LA-LH, and histidine. MDPI 2022-05-23 /pmc/articles/PMC9146156/ /pubmed/35628643 http://dx.doi.org/10.3390/ijms23105834 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Seidel, Elisabeth Christiane
Birkemeyer, Claudia
Baran-Schmidt, Rainer
Meixensberger, Jürgen
Oppermann, Henry
Gaunitz, Frank
Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds
title Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds
title_full Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds
title_fullStr Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds
title_full_unstemmed Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds
title_short Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds
title_sort viability of glioblastoma cells and fibroblasts in the presence of imidazole-containing compounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146156/
https://www.ncbi.nlm.nih.gov/pubmed/35628643
http://dx.doi.org/10.3390/ijms23105834
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