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miR-182-5p Regulates Nogo-A Expression and Promotes Neurite Outgrowth of Hippocampal Neurons In Vitro

Nogo-A protein is a key myelin-associated inhibitor of axonal growth, regeneration, and plasticity in the central nervous system (CNS). Regulation of the Nogo-A/NgR1 pathway facilitates functional recovery and neural repair after spinal cord trauma and ischemic stroke. MicroRNAs are described as eff...

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Autores principales: Soto, Altea, Nieto-Díaz, Manuel, Reigada, David, Barreda-Manso, María Asunción, Muñoz-Galdeano, Teresa, Maza, Rodrigo M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146179/
https://www.ncbi.nlm.nih.gov/pubmed/35631355
http://dx.doi.org/10.3390/ph15050529
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author Soto, Altea
Nieto-Díaz, Manuel
Reigada, David
Barreda-Manso, María Asunción
Muñoz-Galdeano, Teresa
Maza, Rodrigo M.
author_facet Soto, Altea
Nieto-Díaz, Manuel
Reigada, David
Barreda-Manso, María Asunción
Muñoz-Galdeano, Teresa
Maza, Rodrigo M.
author_sort Soto, Altea
collection PubMed
description Nogo-A protein is a key myelin-associated inhibitor of axonal growth, regeneration, and plasticity in the central nervous system (CNS). Regulation of the Nogo-A/NgR1 pathway facilitates functional recovery and neural repair after spinal cord trauma and ischemic stroke. MicroRNAs are described as effective tools for the regulation of important processes in the CNS, such as neuronal differentiation, neuritogenesis, and plasticity. Our results show that miR-182-5p mimic specifically downregulates the expression of the luciferase reporter gene fused to the mouse Nogo-A 3′UTR, and Nogo-A protein expression in Neuro-2a and C6 cells. Finally, we observed that when rat primary hippocampal neurons are co-cultured with C6 cells transfected with miR-182-5p mimic, there is a promotion of the outgrowth of neuronal neurites in length. From all these data, we suggest that miR-182-5p may be a potential therapeutic tool for the promotion of axonal regeneration in different diseases of the CNS.
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spelling pubmed-91461792022-05-29 miR-182-5p Regulates Nogo-A Expression and Promotes Neurite Outgrowth of Hippocampal Neurons In Vitro Soto, Altea Nieto-Díaz, Manuel Reigada, David Barreda-Manso, María Asunción Muñoz-Galdeano, Teresa Maza, Rodrigo M. Pharmaceuticals (Basel) Article Nogo-A protein is a key myelin-associated inhibitor of axonal growth, regeneration, and plasticity in the central nervous system (CNS). Regulation of the Nogo-A/NgR1 pathway facilitates functional recovery and neural repair after spinal cord trauma and ischemic stroke. MicroRNAs are described as effective tools for the regulation of important processes in the CNS, such as neuronal differentiation, neuritogenesis, and plasticity. Our results show that miR-182-5p mimic specifically downregulates the expression of the luciferase reporter gene fused to the mouse Nogo-A 3′UTR, and Nogo-A protein expression in Neuro-2a and C6 cells. Finally, we observed that when rat primary hippocampal neurons are co-cultured with C6 cells transfected with miR-182-5p mimic, there is a promotion of the outgrowth of neuronal neurites in length. From all these data, we suggest that miR-182-5p may be a potential therapeutic tool for the promotion of axonal regeneration in different diseases of the CNS. MDPI 2022-04-25 /pmc/articles/PMC9146179/ /pubmed/35631355 http://dx.doi.org/10.3390/ph15050529 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Soto, Altea
Nieto-Díaz, Manuel
Reigada, David
Barreda-Manso, María Asunción
Muñoz-Galdeano, Teresa
Maza, Rodrigo M.
miR-182-5p Regulates Nogo-A Expression and Promotes Neurite Outgrowth of Hippocampal Neurons In Vitro
title miR-182-5p Regulates Nogo-A Expression and Promotes Neurite Outgrowth of Hippocampal Neurons In Vitro
title_full miR-182-5p Regulates Nogo-A Expression and Promotes Neurite Outgrowth of Hippocampal Neurons In Vitro
title_fullStr miR-182-5p Regulates Nogo-A Expression and Promotes Neurite Outgrowth of Hippocampal Neurons In Vitro
title_full_unstemmed miR-182-5p Regulates Nogo-A Expression and Promotes Neurite Outgrowth of Hippocampal Neurons In Vitro
title_short miR-182-5p Regulates Nogo-A Expression and Promotes Neurite Outgrowth of Hippocampal Neurons In Vitro
title_sort mir-182-5p regulates nogo-a expression and promotes neurite outgrowth of hippocampal neurons in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146179/
https://www.ncbi.nlm.nih.gov/pubmed/35631355
http://dx.doi.org/10.3390/ph15050529
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