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Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets
Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that endangers the swine industry worldwide. Recently, lineage 1 PRRSVs, especially NADC30-like PRRSVs, have become the major endemic strains in many pig-breeding countries. Since 2016, NADC30-like PRRSV has become...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146329/ https://www.ncbi.nlm.nih.gov/pubmed/35632508 http://dx.doi.org/10.3390/vaccines10050752 |
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author | Zhang, Hongliang Xiang, Lirun Xu, Hu Li, Chao Tang, Yan-Dong Gong, Bangjun Zhang, Wenli Zhao, Jing Song, Shuaijie Peng, Jinmei Wang, Qian An, Tongqing Cai, Xuehui Tian, Zhi-Jun |
author_facet | Zhang, Hongliang Xiang, Lirun Xu, Hu Li, Chao Tang, Yan-Dong Gong, Bangjun Zhang, Wenli Zhao, Jing Song, Shuaijie Peng, Jinmei Wang, Qian An, Tongqing Cai, Xuehui Tian, Zhi-Jun |
author_sort | Zhang, Hongliang |
collection | PubMed |
description | Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that endangers the swine industry worldwide. Recently, lineage 1 PRRSVs, especially NADC30-like PRRSVs, have become the major endemic strains in many pig-breeding countries. Since 2016, NADC30-like PRRSV has become the predominant strain in China. Unfortunately, current commercial vaccines cannot provide sufficient protection against this strain. Here, an attenuated lineage 1 PRRSV strain, named SD-R, was obtained by passaging an NADC30-like PRRSV strain SD in Marc-145 cells for 125 passages. Four-week-old PRRSV-free piglets were vaccinated intramuscularly with 10(5.0)TCID(50) SD-R and then challenged intramuscularly (2 mL) and intranasally (2 mL) with homologous NADC30-like PRRSV SD (1 × 10(5.0)TCID(50)/(mL)) and heterologous NADC30-like PRRSV HLJWK108-1711 (1 × 10(5.0)TCID(50)/(mL)). The results showed that antibodies against specific PRRSVs in 5 of 5 immunized piglets were positive after a 14-day post-vaccination and did not develop fever or clinical diseases after NADC30-like PRRSV challenges. Additionally, compared with challenge control piglets, vaccinated piglets gained significantly more weight and showed much milder pathological lesions. Furthermore, the viral replication levels of the immunized group were significantly lower than those of the challenge control group. These results demonstrate that lineage 1 PRRSV SD-R is a good candidate for an efficacious vaccine, providing complete clinical protection for piglets against NADC30-like PRRSVs. |
format | Online Article Text |
id | pubmed-9146329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91463292022-05-29 Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets Zhang, Hongliang Xiang, Lirun Xu, Hu Li, Chao Tang, Yan-Dong Gong, Bangjun Zhang, Wenli Zhao, Jing Song, Shuaijie Peng, Jinmei Wang, Qian An, Tongqing Cai, Xuehui Tian, Zhi-Jun Vaccines (Basel) Article Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that endangers the swine industry worldwide. Recently, lineage 1 PRRSVs, especially NADC30-like PRRSVs, have become the major endemic strains in many pig-breeding countries. Since 2016, NADC30-like PRRSV has become the predominant strain in China. Unfortunately, current commercial vaccines cannot provide sufficient protection against this strain. Here, an attenuated lineage 1 PRRSV strain, named SD-R, was obtained by passaging an NADC30-like PRRSV strain SD in Marc-145 cells for 125 passages. Four-week-old PRRSV-free piglets were vaccinated intramuscularly with 10(5.0)TCID(50) SD-R and then challenged intramuscularly (2 mL) and intranasally (2 mL) with homologous NADC30-like PRRSV SD (1 × 10(5.0)TCID(50)/(mL)) and heterologous NADC30-like PRRSV HLJWK108-1711 (1 × 10(5.0)TCID(50)/(mL)). The results showed that antibodies against specific PRRSVs in 5 of 5 immunized piglets were positive after a 14-day post-vaccination and did not develop fever or clinical diseases after NADC30-like PRRSV challenges. Additionally, compared with challenge control piglets, vaccinated piglets gained significantly more weight and showed much milder pathological lesions. Furthermore, the viral replication levels of the immunized group were significantly lower than those of the challenge control group. These results demonstrate that lineage 1 PRRSV SD-R is a good candidate for an efficacious vaccine, providing complete clinical protection for piglets against NADC30-like PRRSVs. MDPI 2022-05-10 /pmc/articles/PMC9146329/ /pubmed/35632508 http://dx.doi.org/10.3390/vaccines10050752 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Hongliang Xiang, Lirun Xu, Hu Li, Chao Tang, Yan-Dong Gong, Bangjun Zhang, Wenli Zhao, Jing Song, Shuaijie Peng, Jinmei Wang, Qian An, Tongqing Cai, Xuehui Tian, Zhi-Jun Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets |
title | Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets |
title_full | Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets |
title_fullStr | Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets |
title_full_unstemmed | Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets |
title_short | Lineage 1 Porcine Reproductive and Respiratory Syndrome Virus Attenuated Live Vaccine Provides Broad Cross-Protection against Homologous and Heterologous NADC30-Like Virus Challenge in Piglets |
title_sort | lineage 1 porcine reproductive and respiratory syndrome virus attenuated live vaccine provides broad cross-protection against homologous and heterologous nadc30-like virus challenge in piglets |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146329/ https://www.ncbi.nlm.nih.gov/pubmed/35632508 http://dx.doi.org/10.3390/vaccines10050752 |
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