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Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer

Microfluidic devices offer excellent heat transfer, enabling the biochemical reactions to be more efficient. However, the precision of temperature sensing and control of microfluids is limited by the size effect. Here in this work, the relationship between the microfluids and the glass substrate of...

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Autores principales: Meng, Jiyu, Yu, Chengzhuang, Li, Shanshan, Wei, Chunyang, Dai, Shijie, Li, Hui, Li, Junwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146403/
https://www.ncbi.nlm.nih.gov/pubmed/35630259
http://dx.doi.org/10.3390/mi13050792
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author Meng, Jiyu
Yu, Chengzhuang
Li, Shanshan
Wei, Chunyang
Dai, Shijie
Li, Hui
Li, Junwei
author_facet Meng, Jiyu
Yu, Chengzhuang
Li, Shanshan
Wei, Chunyang
Dai, Shijie
Li, Hui
Li, Junwei
author_sort Meng, Jiyu
collection PubMed
description Microfluidic devices offer excellent heat transfer, enabling the biochemical reactions to be more efficient. However, the precision of temperature sensing and control of microfluids is limited by the size effect. Here in this work, the relationship between the microfluids and the glass substrate of a typical microfluidic device is investigated. With an intelligent structure design and liquid metal, we demonstrated that a millimeter-scale industrial temperature sensor could be utilized for temperature sensing of micro-scale fluids. We proposed a heat transfer model based on this design, where the local correlations between the macro-scale temperature sensor and the micro-scale fluids were investigated. As a demonstration, a set of temperature-sensitive nucleic acid amplification tests were taken to show the precision of temperature control for micro-scale reagents. Comparations of theoretical and experimental data further verify the effectiveness of our heat transfer model. With the presented compensation approach, the slight fluorescent intensity changes caused by isothermal amplification polymerase chain reaction (PCR) temperature could be distinguished. For instance, the probability distribution plots of fluorescent intensity are significant from each other, even if the amplification temperature has a difference of 1 °C. Thus, this method may serve as a universal approach for micro–macro interface sensing and is helpful beyond microfluidic applications.
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spelling pubmed-91464032022-05-29 Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer Meng, Jiyu Yu, Chengzhuang Li, Shanshan Wei, Chunyang Dai, Shijie Li, Hui Li, Junwei Micromachines (Basel) Article Microfluidic devices offer excellent heat transfer, enabling the biochemical reactions to be more efficient. However, the precision of temperature sensing and control of microfluids is limited by the size effect. Here in this work, the relationship between the microfluids and the glass substrate of a typical microfluidic device is investigated. With an intelligent structure design and liquid metal, we demonstrated that a millimeter-scale industrial temperature sensor could be utilized for temperature sensing of micro-scale fluids. We proposed a heat transfer model based on this design, where the local correlations between the macro-scale temperature sensor and the micro-scale fluids were investigated. As a demonstration, a set of temperature-sensitive nucleic acid amplification tests were taken to show the precision of temperature control for micro-scale reagents. Comparations of theoretical and experimental data further verify the effectiveness of our heat transfer model. With the presented compensation approach, the slight fluorescent intensity changes caused by isothermal amplification polymerase chain reaction (PCR) temperature could be distinguished. For instance, the probability distribution plots of fluorescent intensity are significant from each other, even if the amplification temperature has a difference of 1 °C. Thus, this method may serve as a universal approach for micro–macro interface sensing and is helpful beyond microfluidic applications. MDPI 2022-05-19 /pmc/articles/PMC9146403/ /pubmed/35630259 http://dx.doi.org/10.3390/mi13050792 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meng, Jiyu
Yu, Chengzhuang
Li, Shanshan
Wei, Chunyang
Dai, Shijie
Li, Hui
Li, Junwei
Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer
title Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer
title_full Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer
title_fullStr Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer
title_full_unstemmed Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer
title_short Microfluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer
title_sort microfluidics temperature compensating and monitoring based on liquid metal heat transfer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146403/
https://www.ncbi.nlm.nih.gov/pubmed/35630259
http://dx.doi.org/10.3390/mi13050792
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