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Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021

In recent years, the prevalence of pseudorabies virus (PRV) has caused huge economic losses to the Chinese pig industry. Meanwhile, PRV infection in humans also sounded the alarm about its cross-species transmission from pigs to humans. To study the regional PRV epidemic, serological and epidemiolog...

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Autores principales: Yao, Jun, Li, Juan, Gao, Lin, He, Yuwen, Xie, Jiarui, Zhu, Pei, Zhang, Ying, Zhang, Xue, Duan, Luoyan, Yang, Shibiao, Song, Chunlian, Shu, Xianghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146497/
https://www.ncbi.nlm.nih.gov/pubmed/35632637
http://dx.doi.org/10.3390/v14050895
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author Yao, Jun
Li, Juan
Gao, Lin
He, Yuwen
Xie, Jiarui
Zhu, Pei
Zhang, Ying
Zhang, Xue
Duan, Luoyan
Yang, Shibiao
Song, Chunlian
Shu, Xianghua
author_facet Yao, Jun
Li, Juan
Gao, Lin
He, Yuwen
Xie, Jiarui
Zhu, Pei
Zhang, Ying
Zhang, Xue
Duan, Luoyan
Yang, Shibiao
Song, Chunlian
Shu, Xianghua
author_sort Yao, Jun
collection PubMed
description In recent years, the prevalence of pseudorabies virus (PRV) has caused huge economic losses to the Chinese pig industry. Meanwhile, PRV infection in humans also sounded the alarm about its cross-species transmission from pigs to humans. To study the regional PRV epidemic, serological and epidemiological investigations of PRV in pig populations from Yunnan Province during 2017–2021 were performed. The results showed that 31.37% (6324/20,158, 95% CI 30.73–32.01) of serum samples were positive for PRV glycoprotein E (gE)-specific antibodies via enzyme-linked immunosorbent assay (ELISA). The risk factors, including the breeding scale and development stage, were significantly associated with PRV seroprevalence among pigs in Yunnan Province. Of the 416 tissue samples collected from PRV-suspected pigs in Yunnan Province, 43 (10.33%, 95% CI 7.41–13.26) samples were positive for PRV-gE nucleic acid in which 15 novel PRV strains from these PRV-positive samples were isolated, whose gC and gE sequences were analyzed. Phylogenetic analysis showed that all 15 isolates obtained in this study belonged to the genotype II. Additionally, the gC gene of one isolate (YuN-YL-2017) was genetically closer to variant PRV strains compared with others, while the gE gene was in the same clade with other classical PRV strains, indicating that this isolate might be a recombinant strain generated from the classical and variant strains. The results revealed the severe PRV epidemic in Yunnan Province and indicated that PRV variants are the major genotypes threatening the pig industry development.
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spelling pubmed-91464972022-05-29 Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021 Yao, Jun Li, Juan Gao, Lin He, Yuwen Xie, Jiarui Zhu, Pei Zhang, Ying Zhang, Xue Duan, Luoyan Yang, Shibiao Song, Chunlian Shu, Xianghua Viruses Article In recent years, the prevalence of pseudorabies virus (PRV) has caused huge economic losses to the Chinese pig industry. Meanwhile, PRV infection in humans also sounded the alarm about its cross-species transmission from pigs to humans. To study the regional PRV epidemic, serological and epidemiological investigations of PRV in pig populations from Yunnan Province during 2017–2021 were performed. The results showed that 31.37% (6324/20,158, 95% CI 30.73–32.01) of serum samples were positive for PRV glycoprotein E (gE)-specific antibodies via enzyme-linked immunosorbent assay (ELISA). The risk factors, including the breeding scale and development stage, were significantly associated with PRV seroprevalence among pigs in Yunnan Province. Of the 416 tissue samples collected from PRV-suspected pigs in Yunnan Province, 43 (10.33%, 95% CI 7.41–13.26) samples were positive for PRV-gE nucleic acid in which 15 novel PRV strains from these PRV-positive samples were isolated, whose gC and gE sequences were analyzed. Phylogenetic analysis showed that all 15 isolates obtained in this study belonged to the genotype II. Additionally, the gC gene of one isolate (YuN-YL-2017) was genetically closer to variant PRV strains compared with others, while the gE gene was in the same clade with other classical PRV strains, indicating that this isolate might be a recombinant strain generated from the classical and variant strains. The results revealed the severe PRV epidemic in Yunnan Province and indicated that PRV variants are the major genotypes threatening the pig industry development. MDPI 2022-04-25 /pmc/articles/PMC9146497/ /pubmed/35632637 http://dx.doi.org/10.3390/v14050895 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yao, Jun
Li, Juan
Gao, Lin
He, Yuwen
Xie, Jiarui
Zhu, Pei
Zhang, Ying
Zhang, Xue
Duan, Luoyan
Yang, Shibiao
Song, Chunlian
Shu, Xianghua
Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021
title Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021
title_full Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021
title_fullStr Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021
title_full_unstemmed Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021
title_short Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021
title_sort epidemiological investigation and genetic analysis of pseudorabies virus in yunnan province of china from 2017 to 2021
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146497/
https://www.ncbi.nlm.nih.gov/pubmed/35632637
http://dx.doi.org/10.3390/v14050895
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