Cargando…

Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa

The soil microbiome contributes to nutrient acquisition and plant adaptation to numerous biotic and abiotic stresses. Numerous studies have been conducted over the past decade showing that plants take up nutrients better when associated with fungi and additional beneficial bacteria that promote plan...

Descripción completa

Detalles Bibliográficos
Autores principales: Bandyopadhyay, Prasun, Yadav, Bal Govind, Kumar, Srinivasan Ganesh, Kumar, Rahul, Kogel, Karl-Heinz, Kumar, Shashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146537/
https://www.ncbi.nlm.nih.gov/pubmed/35628709
http://dx.doi.org/10.3390/jof8050453
_version_ 1784716587617484800
author Bandyopadhyay, Prasun
Yadav, Bal Govind
Kumar, Srinivasan Ganesh
Kumar, Rahul
Kogel, Karl-Heinz
Kumar, Shashi
author_facet Bandyopadhyay, Prasun
Yadav, Bal Govind
Kumar, Srinivasan Ganesh
Kumar, Rahul
Kogel, Karl-Heinz
Kumar, Shashi
author_sort Bandyopadhyay, Prasun
collection PubMed
description The soil microbiome contributes to nutrient acquisition and plant adaptation to numerous biotic and abiotic stresses. Numerous studies have been conducted over the past decade showing that plants take up nutrients better when associated with fungi and additional beneficial bacteria that promote plant growth, but the mechanisms by which the plant host benefits from this tripartite association are not yet fully understood. In this article, we report on a synergistic interaction between rice (Oryza sativa), Piriformospora indica (an endophytic fungus colonizing the rice roots), and Azotobacter chroococcum strain W5, a free-living nitrogen-fixing bacterium. On the basis of mRNA expression analysis and enzymatic activity, we found that co-inoculation of plant roots with the fungus and the rhizobacterium leads to enhanced plant growth and improved nutrient uptake compared to inoculation with either of the two microbes individually. Proteome analysis of O. sativa further revealed that proteins involved in nitrogen and phosphorus metabolism are upregulated and improve nitrogen and phosphate uptake. Our results also show that A. chroococcum supports colonization of rice roots by P. indica, and consequentially, the plants are more resistant to biotic stress upon co-colonization. Our research provides detailed insights into the mechanisms by which microbial partners synergistically promote each other in the interaction while being associated with the host plant.
format Online
Article
Text
id pubmed-9146537
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91465372022-05-29 Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa Bandyopadhyay, Prasun Yadav, Bal Govind Kumar, Srinivasan Ganesh Kumar, Rahul Kogel, Karl-Heinz Kumar, Shashi J Fungi (Basel) Article The soil microbiome contributes to nutrient acquisition and plant adaptation to numerous biotic and abiotic stresses. Numerous studies have been conducted over the past decade showing that plants take up nutrients better when associated with fungi and additional beneficial bacteria that promote plant growth, but the mechanisms by which the plant host benefits from this tripartite association are not yet fully understood. In this article, we report on a synergistic interaction between rice (Oryza sativa), Piriformospora indica (an endophytic fungus colonizing the rice roots), and Azotobacter chroococcum strain W5, a free-living nitrogen-fixing bacterium. On the basis of mRNA expression analysis and enzymatic activity, we found that co-inoculation of plant roots with the fungus and the rhizobacterium leads to enhanced plant growth and improved nutrient uptake compared to inoculation with either of the two microbes individually. Proteome analysis of O. sativa further revealed that proteins involved in nitrogen and phosphorus metabolism are upregulated and improve nitrogen and phosphate uptake. Our results also show that A. chroococcum supports colonization of rice roots by P. indica, and consequentially, the plants are more resistant to biotic stress upon co-colonization. Our research provides detailed insights into the mechanisms by which microbial partners synergistically promote each other in the interaction while being associated with the host plant. MDPI 2022-04-27 /pmc/articles/PMC9146537/ /pubmed/35628709 http://dx.doi.org/10.3390/jof8050453 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bandyopadhyay, Prasun
Yadav, Bal Govind
Kumar, Srinivasan Ganesh
Kumar, Rahul
Kogel, Karl-Heinz
Kumar, Shashi
Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa
title Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa
title_full Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa
title_fullStr Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa
title_full_unstemmed Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa
title_short Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa
title_sort piriformospora indica and azotobacter chroococcum consortium facilitates higher acquisition of n, p with improved carbon allocation and enhanced plant growth in oryza sativa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146537/
https://www.ncbi.nlm.nih.gov/pubmed/35628709
http://dx.doi.org/10.3390/jof8050453
work_keys_str_mv AT bandyopadhyayprasun piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa
AT yadavbalgovind piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa
AT kumarsrinivasanganesh piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa
AT kumarrahul piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa
AT kogelkarlheinz piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa
AT kumarshashi piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa