Cargando…
Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa
The soil microbiome contributes to nutrient acquisition and plant adaptation to numerous biotic and abiotic stresses. Numerous studies have been conducted over the past decade showing that plants take up nutrients better when associated with fungi and additional beneficial bacteria that promote plan...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146537/ https://www.ncbi.nlm.nih.gov/pubmed/35628709 http://dx.doi.org/10.3390/jof8050453 |
_version_ | 1784716587617484800 |
---|---|
author | Bandyopadhyay, Prasun Yadav, Bal Govind Kumar, Srinivasan Ganesh Kumar, Rahul Kogel, Karl-Heinz Kumar, Shashi |
author_facet | Bandyopadhyay, Prasun Yadav, Bal Govind Kumar, Srinivasan Ganesh Kumar, Rahul Kogel, Karl-Heinz Kumar, Shashi |
author_sort | Bandyopadhyay, Prasun |
collection | PubMed |
description | The soil microbiome contributes to nutrient acquisition and plant adaptation to numerous biotic and abiotic stresses. Numerous studies have been conducted over the past decade showing that plants take up nutrients better when associated with fungi and additional beneficial bacteria that promote plant growth, but the mechanisms by which the plant host benefits from this tripartite association are not yet fully understood. In this article, we report on a synergistic interaction between rice (Oryza sativa), Piriformospora indica (an endophytic fungus colonizing the rice roots), and Azotobacter chroococcum strain W5, a free-living nitrogen-fixing bacterium. On the basis of mRNA expression analysis and enzymatic activity, we found that co-inoculation of plant roots with the fungus and the rhizobacterium leads to enhanced plant growth and improved nutrient uptake compared to inoculation with either of the two microbes individually. Proteome analysis of O. sativa further revealed that proteins involved in nitrogen and phosphorus metabolism are upregulated and improve nitrogen and phosphate uptake. Our results also show that A. chroococcum supports colonization of rice roots by P. indica, and consequentially, the plants are more resistant to biotic stress upon co-colonization. Our research provides detailed insights into the mechanisms by which microbial partners synergistically promote each other in the interaction while being associated with the host plant. |
format | Online Article Text |
id | pubmed-9146537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91465372022-05-29 Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa Bandyopadhyay, Prasun Yadav, Bal Govind Kumar, Srinivasan Ganesh Kumar, Rahul Kogel, Karl-Heinz Kumar, Shashi J Fungi (Basel) Article The soil microbiome contributes to nutrient acquisition and plant adaptation to numerous biotic and abiotic stresses. Numerous studies have been conducted over the past decade showing that plants take up nutrients better when associated with fungi and additional beneficial bacteria that promote plant growth, but the mechanisms by which the plant host benefits from this tripartite association are not yet fully understood. In this article, we report on a synergistic interaction between rice (Oryza sativa), Piriformospora indica (an endophytic fungus colonizing the rice roots), and Azotobacter chroococcum strain W5, a free-living nitrogen-fixing bacterium. On the basis of mRNA expression analysis and enzymatic activity, we found that co-inoculation of plant roots with the fungus and the rhizobacterium leads to enhanced plant growth and improved nutrient uptake compared to inoculation with either of the two microbes individually. Proteome analysis of O. sativa further revealed that proteins involved in nitrogen and phosphorus metabolism are upregulated and improve nitrogen and phosphate uptake. Our results also show that A. chroococcum supports colonization of rice roots by P. indica, and consequentially, the plants are more resistant to biotic stress upon co-colonization. Our research provides detailed insights into the mechanisms by which microbial partners synergistically promote each other in the interaction while being associated with the host plant. MDPI 2022-04-27 /pmc/articles/PMC9146537/ /pubmed/35628709 http://dx.doi.org/10.3390/jof8050453 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bandyopadhyay, Prasun Yadav, Bal Govind Kumar, Srinivasan Ganesh Kumar, Rahul Kogel, Karl-Heinz Kumar, Shashi Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa |
title | Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa |
title_full | Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa |
title_fullStr | Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa |
title_full_unstemmed | Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa |
title_short | Piriformospora indica and Azotobacter chroococcum Consortium Facilitates Higher Acquisition of N, P with Improved Carbon Allocation and Enhanced Plant Growth in Oryza sativa |
title_sort | piriformospora indica and azotobacter chroococcum consortium facilitates higher acquisition of n, p with improved carbon allocation and enhanced plant growth in oryza sativa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146537/ https://www.ncbi.nlm.nih.gov/pubmed/35628709 http://dx.doi.org/10.3390/jof8050453 |
work_keys_str_mv | AT bandyopadhyayprasun piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa AT yadavbalgovind piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa AT kumarsrinivasanganesh piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa AT kumarrahul piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa AT kogelkarlheinz piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa AT kumarshashi piriformosporaindicaandazotobacterchroococcumconsortiumfacilitateshigheracquisitionofnpwithimprovedcarbonallocationandenhancedplantgrowthinoryzasativa |